Effects of local injection and intravenous injection of allogeneic bone marrow mesenchymal stem cells on the structure and function of damaged anal sphincter in rats

2020 ◽  
Vol 14 (7) ◽  
pp. 989-1000
Author(s):  
Peng Li ◽  
Xiaoying Ma ◽  
Wenqi Jin ◽  
Xiaojia Li ◽  
Jie Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Anal Sphincter ◽  
Intravenous Injection ◽  
Structure And Function ◽  
Local Injection ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells ◽  
And Function

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

2015 ◽  
Vol 99 (8) ◽  
pp. 1681-1690 ◽  
Author(s):  
Aurelio Vega ◽  
Miguel Angel Martín-Ferrero ◽  
Francisco Del Canto ◽  
Mercedes Alberca ◽  
Veronica García ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Knee Osteoarthritis ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis

2007 ◽  
Vol 56 (4) ◽  
pp. 1175-1186 ◽  
Author(s):  
Andrea Augello ◽  
Roberta Tasso ◽  
Simone Maria Negrini ◽  
Ranieri Cancedda ◽  
Giuseppina Pennesi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Tissue Damage ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Collagen Induced Arthritis ◽  
Marrow Mesenchymal Stem Cells

scholarly journals In VivoTracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Juan Cao ◽  
Shike Hou ◽  
Hui Ding ◽  
Ziquan Liu ◽  
Meijuan Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bioluminescence Imaging ◽  
Ex Vivo ◽  
Allogeneic Bone Marrow ◽  
Therapeutic Effects ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells

Recently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dynamic distribution of MSCs afterin vivoadministration. In this study, we track systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in normal rats through bioluminescence imaging (BLI) in real time.Ex vivoorgan imaging, immunohistochemistry (IHC), and RT-PCR were conducted to verify the histological distribution of BMSCs. Our results showed that BMSCs home to the dorsal skin apart from the lungs and kidneys after tail vein injection and could not be detected 14 days later. Allogeneic BMSCs mainly appeared not at the parenchymatous organs but at the subepidermal connective tissue and adipose tissue in healthy rats. There were no significant MSCs-related adverse effects except for transient decrease in neutrophils. These findings will provide experimental evidences for a better understanding of the biocharacteristics of BMSCs.

Survival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis

2014 ◽  
Vol 27 (03) ◽  
pp. 204-209 ◽  
Author(s):  
F. Staffieri ◽  
G. Rossi ◽  
E. Francioso ◽  
A. Crovace ◽  
L. Lacitignola
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Fibrin Glue ◽  
Fluorescent Protein ◽  
Allogeneic Bone ◽  
Red Fluorescent Protein ◽  
Ovine Model ◽  
Cd34 Cells ◽  
Marrow Mesenchymal Stem Cells

SummaryObjective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BMMSCRFP) in an ovine model of collagenase-induced tendinopathy.Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contralateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC.Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP.Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment.

scholarly journals Intrathecal Transplantation of Autologous and Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Dogs

2021 ◽  
Vol 30 ◽  
pp. 096368972110344
Author(s):  
Felipe Pérez Benavides ◽  
Giovana Boff Araujo Pinto ◽  
Marta Cristina Thomas Heckler ◽  
Diana Milena Rodríguez Hurtado ◽  
Livia Ramos Teixeira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Treatment Success ◽  
Control Group ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells ◽  
Intrathecal Transplantation ◽  
Healthy Dogs ◽  
Magnetic Resonance Imaging Mri

The route used in the transplantation of mesenchymal stem cells (MSCs) can directly affect the treatment success. The transplantation of MSCs via the intrathecal (IT) route can be an important therapeutic strategy for neurological disorders. The objective of this study was to evaluate the safety and feasibility of the IT transplantation of autologous (Auto-MSCs) and allogeneic (Allo-MSCs) bone marrow mesenchymal stem cells (BM-MSCs) in healthy dogs. Based on neurodisability score, cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI), no significant differences from the control group were observed on day 1 or day 5 after IT Auto- or Allo-MSCs transplantation ( P > 0.05). In addition, analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression in the CSF revealed no significant differences ( P > 0.05) at 5 days after IT transplantation in the Auto- or Allo-MSCs group when compared to the control. Intrathecal transplantation of BM-MSCs in dogs provides a safe, easy and minimally invasive route for the use of cell-based therapeutics in central nervous system diseases.

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