Cell culture of differentiated human salivary epithelial cells in a serum‐free and scalable suspension system: The salivary functional units model

2019 ◽  
Vol 13 (9) ◽  
pp. 1559-1570 ◽  
Author(s):  
You Jung Seo ◽  
Maria Alberta Lilliu ◽  
Ghada Abu Elghanam ◽  
Thomas T. Nguyen ◽  
Younan Liu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Suspension System ◽  
Serum Free ◽  
Functional Units ◽  
Salivary Epithelial Cells

scholarly journals Development of Microfluidic, Serum-Free Bronchial Epithelial Cells-on-a-Chip to Facilitate a More Realistic In vitro Testing of Nanoplastics

2021 ◽  
Vol 3 ◽  
Author(s):  
Govind Gupta ◽  
Srikanth Vallabani ◽  
Romain Bordes ◽  
Kunal Bhattacharya ◽  
Bengt Fadeel
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Cellular Uptake ◽  
Culture Conditions ◽  
Bronchial Epithelial Cells ◽  
The Body ◽  
Bronchial Epithelial ◽  
Serum Free ◽  
Static Conditions

Most cell culture models are static, but the cellular microenvironment in the body is dynamic. Here, we established a microfluidic-based in vitro model of human bronchial epithelial cells in which cells are stationary, but nutrient supply is dynamic, and we used this system to evaluate cellular uptake of nanoparticles. The cells were maintained in fetal calf serum-free and bovine pituitary extract-free cell culture medium. BEAS-2B, an immortalized, non-tumorigenic human cell line, was used as a model and the cells were grown in a chip within a microfluidic device and were briefly infused with amorphous silica (SiO2) nanoparticles or polystyrene (PS) nanoparticles of similar primary sizes but with different densities. For comparison, tests were also performed using static, multi-well cultures. Cellular uptake of the fluorescently labeled particles was investigated by flow cytometry and confocal microscopy. Exposure under dynamic culture conditions resulted in higher cellular uptake of the PS nanoparticles when compared to static conditions, while uptake of SiO2 nanoparticles was similar in both settings. The present study has shown that it is feasible to grow human lung cells under completely animal-free conditions using a microfluidic-based device, and we have also found that cellular uptake of PS nanoparticles aka nanoplastics is highly dependent on culture conditions. Hence, traditional cell cultures may not accurately reflect the uptake of low-density particles, potentially leading to an underestimation of their cellular impact.

scholarly journals SUMOylation Attenuates Sensitivity toward Hypoxia- or Desferroxamine-Induced Injury by Modulating Adaptive Responses in Salivary Epithelial Cells

2006 ◽  
Vol 168 (5) ◽  
pp. 1452-1463 ◽  
Author(s):  
Ha-Van Nguyen ◽  
Jo-Lin Chen ◽  
Jenny Zhong ◽  
Kwang-Jin Kim ◽  
Edward D. Crandall ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Adaptive Responses ◽  
Salivary Epithelial Cells

Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology

1991 ◽  
Vol 125 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Bertil G. Casslén ◽  
Michael J. K. Harper
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Collagen Matrix ◽  
Human Endometrium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Endometrial Stromal Cells ◽  
Prolonged Incubation ◽  
Serum Free ◽  
Endometrial Epithelial Cells

Abstract. The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2α (PGF2α) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2α by epithelial cells. We conclude that the secretion of PGF2α from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.

scholarly journals P2X7 Receptor-mediated Membrane Blebbing in Salivary Epithelial Cells

2009 ◽  
Vol 13 (3) ◽  
pp. 175 ◽  
Author(s):  
Sung-Min Hwang ◽  
Na-Youn Koo ◽  
Se-Young Choi ◽  
Gae-Sig Chun ◽  
Joong-Soo Kim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
P2x7 Receptor ◽  
Membrane Blebbing ◽  
Salivary Epithelial Cells

Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

2007 ◽  
Vol 40 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard ◽  
Jehangir Mistry ◽  
Madhur K. Sinha
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Human Insulin ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free
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