Substrate elasticity induces quiescence and promotes neurogenesis of primary neural stem cells—A biophysical in vitro model of the physiological cerebral milieu

2019 ◽  
Author(s):  
Stefan Blaschke ◽  
Sabine Ulrike Vay ◽  
Niklas Pallast ◽  
Monika Rabenstein ◽  
Jella‐Andrea Abraham ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Vitro Model ◽  
Substrate Elasticity

scholarly journals Impaired functions of neural stem cells by abnormal nitric oxide-mediated signaling in an in vitro model of Niemann-Pick type C disease

Cell Research ◽  
2008 ◽  
Vol 18 (6) ◽  
pp. 686-694 ◽  
Author(s):  
Sun-Jung Kim ◽  
Myung-Sin Lim ◽  
Soo-Kyung Kang ◽  
Yong-Soon Lee ◽  
Kyung-Sun Kang
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Type C ◽  
Vitro Model ◽  
Niemann Pick

scholarly journals ATM-deficient human neural stem cells as an in vitro model system to study neurodegeneration

DNA Repair ◽  
2013 ◽  
Vol 12 (8) ◽  
pp. 605-611 ◽  
Author(s):  
Luigi Carlessi ◽  
Elena Fusar Poli ◽  
Lidia De Filippis ◽  
Domenico Delia
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
Human Neural Stem Cells ◽  
In Vitro Model System ◽  
Vitro Model

T1736 An In Vitro Model to Study the Potential of Neural Stem Cells of Diverse Origins to Assume an Enteric Phenotype

2010 ◽  
Vol 138 (5) ◽  
pp. S-567-S-568 ◽  
Author(s):  
Subhash Kulkarni ◽  
Maria-Adelaide Micci ◽  
Gunjan Tiwari ◽  
Xinmin S. Xie ◽  
Pankaj J. Pasricha
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Model ◽  
Vitro Model

A scaffold-free in vitro model for osteogenesis of human mesenchymal stem cells

2011 ◽  
Vol 43 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Cornelia Hildebrandt ◽  
Heiko Büth ◽  
Hagen Thielecke
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Human Mesenchymal Stem Cells ◽  
Vitro Model

scholarly journals Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells

2016 ◽  
Vol 364 (3) ◽  
pp. 573-584 ◽  
Author(s):  
Patrick Wuchter ◽  
Rainer Saffrich ◽  
Stefan Giselbrecht ◽  
Cordula Nies ◽  
Hanna Lorig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
Bone Marrow Niche ◽  
Hematopoietic Stem ◽  
In Vitro Model System ◽  
Vitro Model

Features of the in vitro expression profile of hippocampal neurogenic niche cells during optogenetic stimulation

2021 ◽  
Vol 67 (1) ◽  
pp. 34-41
Author(s):  
E.D. Khilazheva ◽  
A.V. Morgun ◽  
E.B. Boytsova ◽  
A.I. Mosiagina ◽  
A.N. Shuvaev ◽  
...  
Keyword(s):  
Stem Cells ◽  
Expression Profile ◽  
In Vitro Model ◽  
Postnatal Period ◽  
Proliferating Cells ◽  
Aberrant Expression ◽  
Intercellular Interactions ◽  
Neurogenic Niche ◽  
Vitro Model

In the central nervous system of mammals, there are specialized areas in which neurogenesis — neurogenic niches — is observed in the postnatal period. It is believed that astrocytes in the composition of neurogenic niches play a significant role in the regulation of neurogenesis, and therefore they are considered as a promising “target” for the possible control of neurogenesis, including the use of optogenetics. In the framework of this work, we formed an in vitro model of a neurogenic niche, consisting of cerebral endothelial cells, astrocytes and neurospheres. Astrocytes in the neurogenic niche model expressed canalorodopsin ChR2 and underwent photoactivation. The effect of photoactivated astrocytes on the expression profile of neurogenic niche cells was evaluated using immunocytochemical analysis methods. It was found that intact astrocytes in the composition of the neurogenic niche contribute to neuronal differentiation of stem cells, as well as the activation of astroglia expressing photosensitive proteins, changes the expression of molecules characterized by intercellular interactions of pools of resting and proliferating cells in the composition of the neurogenic niche with the participation of NAD+ (Cx43, CD38, CD157), lactate (MCT1). In particular, the registered changes reflect a violation of the paracrine intercellular interactions of two subpopulations of cells, one of which acts as a source of NAD+, and the second as a consumer of NAD+ to ensure the processes of intracellular signal transduction; a change in the mechanisms of lactate transport due to aberrant expression of the lactate transporter MCT1 in cells forming a pool of cells developing along the neuronal path of differentiation. In general, with photostimulation of niche astrocytes, the total proliferative activity increases mainly due to neural progenitor cells, but not neural stem cells. Thus, optogenetic activation of astrocytes can become a promising tool for controlling the activity of neurogenesis processes and the formation of a local proneurogenic microenvironment in an in vitro model of a neurogenic niche.

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