scholarly journals Non‐destructive detection of matrix stabilization correlates with enhanced mechanical properties of self‐assembled articular cartilage

2019 ◽  
Vol 13 (4) ◽  
pp. 637-648 ◽  
Author(s):  
Anne K. Haudenschild ◽  
Benjamin E. Sherlock ◽  
Xiangnan Zhou ◽  
Jerry C. Hu ◽  
J. Kent Leach ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Matrix Stabilization ◽  
Self Assembled ◽  
Non Destructive

Effect of Compressive Load on the Mechanical Property of a Stem Cell Based Self-Assembled Tissue Derived From Synovium

2009 ◽  
Author(s):  
Ryo Emura ◽  
Atsushi Ogawa ◽  
Kei Saito ◽  
Wataru Ando ◽  
Norimasa Nakamura ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Mechanical Property ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Compressive Load ◽  
Static Compression ◽  
Cartilage Injuries ◽  
Immunological Effects ◽  
Self Assembled

Articular cartilage has superior functions such as impact absorption and low friction, although their healing capacities are limited. It is one of potential options for the repair of articular cartilage to use cell-based therapies. We have been developing a novel tissue-engineering technique for the repair of cartilage which involves a stem cell-based self-assembled tissue (scSAT) derived from synovium. As the scSAT is a scaffold-free contrust composed of cells with their native extracellular matrix, it is free from concern regarding long-term immunological effects. The scSAT is expressed as tissue engineered construct (TEC) when it is used for cartilage repair. Previous studies indicated that the mechanical properties of cartilage-like tissues repaired using the scSAT were slightly inferiorer to those of normal cartilage. We have a hypothesis that the mechanical properties of the cartilage-like tissues are improved if the scSAT is subjected to an adequate compressive stimulation in vitro before implantation. The present study was conducted as a preliminary study to determine whether static compression improves the mechanical property of the scSAT for more advanced regenerative medicine to cartilage injuries and degeneration.

scholarly journals Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties

Cartilage ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 480-490 ◽  
Author(s):  
James P. Karchner ◽  
Farzad Yousefi ◽  
Stephanie R. Bitman ◽  
Kurosh Darvish ◽  
Nancy Pleshko
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Near Infrared ◽  
Fiber Optic ◽  
Weight Bearing ◽  
Short Term ◽  
Low Weight ◽  
Proteoglycan Content ◽  
Non Destructive ◽  
Femoral Condyles

Objective Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment. Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions. Design AC plugs ( N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region. Near-infrared (NIR) and mid-infrared (MIR) fiber optic spectra were collected from plugs, and indentation tests were performed to determine the short-term and equilibrium moduli, followed by gravimetric water and biochemical analysis. Results LWB tissues had a significantly greater amount of water determined by NIR and gravimetric assay. The moduli generally increased in tissues from the patellofemoral groove to the condyles, with HWB condyle cartilage having significantly higher moduli. A greater amount of proteoglycan content was also found in HWB tissues, but no differences in collagen content. In addition, NIR-determined water correlated with short-term modulus and proteoglycan content ( R = −0.40 and −0.31, respectively), and a multivariate model with NIR data was able to predict short-term modulus within 15% error. Conclusions The properties of tissues from LWB regions differ from HWB tissues and can be determined non-destructively by infrared fiber optic spectroscopy. Clinicians may be able to use this modality to assess AC prior to harvesting osteochondral grafts for focal defect repair.

Sequential changes in the mechanical properties of viable articular cartilage storedin vitro

1984 ◽  
Vol 2 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Victor J. Thomas ◽  
Sergio A. Jimenez ◽  
Carl T. Brighton ◽  
Norman Brown
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage

Transversely isotropic micromechanics model to determine effect of collagen fibre angle in mechanical properties of articular cartilage

2014 ◽  
Vol 29 (6) ◽  
pp. 377-383 ◽  
Author(s):  
S. Mohammad Mehdi Elhamian ◽  
M. Alizadeh ◽  
M. Mehrdad Shokrieh ◽  
A. Karimi ◽  
S. Pejman Madani
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Collagen Fibre ◽  
Transversely Isotropic ◽  
Micromechanics Model ◽  
Fibre Angle ◽  
Determine Effect

Permeability of Human Glenohumeral Joint Cartilage

1999 ◽  
Author(s):  
Anna Stankiewicz ◽  
Gerard A. Ateshian ◽  
Louis U. Bigliani ◽  
Van C. Mow
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Interstitial Fluid ◽  
Glenohumeral Joint ◽  
Solid Matrix ◽  
Joint Cartilage ◽  
Frictional Drag ◽  
Diarthrodial Joints ◽  
Flow Through ◽  
Fluid Pressurization

Abstract The nearly frictionless lubrication in diarthrodial joints and load support within articular cartilage depends on its mechanical properties. It has been shown that the majority of applied loads on cartilage are supported by interstitial fluid pressurization (Ateshian et al., 1994) which results from the frictional drag of flow through the porous permeable solid matrix. The duration and magnitude of this pressurization are a function of the permeability of cartilage (Lai et al., 1981).

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