scholarly journals Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering

2017 ◽  
Vol 12 (1) ◽  
pp. e159-e170 ◽  
Author(s):  
Benjamin B. Rothrauff ◽  
Luca Coluccino ◽  
Riccardo Gottardi ◽  
Luca Ceseracciu ◽  
Silvia Scaglione ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Extracellular Matrix ◽  
And Cartilage

Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering

2012 ◽  
Vol 113 (5) ◽  
pp. 647-653 ◽  
Author(s):  
Shuaijun Jia ◽  
Lie Liu ◽  
Weimin Pan ◽  
Guolin Meng ◽  
Chunguang Duan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Extracellular Matrix

scholarly journals Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering

2021 ◽  
pp. 088532822110240
Author(s):  
Nastaran Shojarazavi ◽  
Shohreh Mashayekhan ◽  
Hossein Pazooki ◽  
Sadaf Mohsenifard ◽  
Hossein Baniasadi
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Polymer Network ◽  
Cartilage Tissue Engineering ◽  
Compression Modulus ◽  
Interpenetrating Polymer Network ◽  
Cartilage Tissue ◽  
Cartilage Extracellular Matrix ◽  
Capacity Degradation ◽  
Alginate Concentration

In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was optimized to reach a desired mechanical stiffness. Accordingly, the concentrations of alginate and SFN to have an optimum compression modulus in the hydrogel were found to be 1.685 and 1.724% w/v, respectively. The gelation time was found to be about 10 s for all the samples. Scanning electron microscope (SEM) images showed homogeneous dispersion of the SFN in the hydrogel, mimicking the natural cartilage environment. Furthermore, water uptake capacity, degradation rate, cell cytotoxicity, and glycosaminoglycan and collagen II secretions were determined for the optimum hydrogel to support its potential as an injectable scaffold for articular cartilage defects.

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

Starch-polycaprolactone based scaffolds in bone and cartilage tissue engineering approaches

2008 ◽  
pp. 337-356
Author(s):  
M.E. GOMES ◽  
J.T. OLIVEIRA ◽  
M.T. RODRIGUES ◽  
M.I. SANTOS ◽  
K. TUZLAKOGLU ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
And Cartilage
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