Poly(N -isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering

2016 ◽  
Vol 104 (11) ◽  
pp. 2764-2774 ◽  
Author(s):  
Amir Mellati ◽  
Meisam Valizadeh Kiamahalleh ◽  
S. Hadi Madani ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Stem Cell Culture ◽  
Chitosan Scaffold ◽  
And Cartilage

scholarly journals Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering

RSC Advances ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2870-2876 ◽  
Author(s):  
Luyu Wang ◽  
Jinrui Li ◽  
Dan Zhang ◽  
Shanshan Ma ◽  
Junni Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Hyaluronic Acid ◽  
Potential Application ◽  
Three Dimensional ◽  
Stem Cell Culture ◽  
Hyaluronic Acid Hydrogel

A new hyaluronic acid hydrogel dual-enzymatically cross-linked by HRP and GalOX and application for three-dimensional stem cell culture and tissue engineering.

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

scholarly journals Microfluidic systems for stem cell-based neural tissue engineering

Lab on a Chip ◽  
2016 ◽  
Vol 16 (14) ◽  
pp. 2551-2571 ◽  
Author(s):  
Mahdi Karimi ◽  
Sajad Bahrami ◽  
Hamed Mirshekari ◽  
Seyed Masoud Moosavi Basri ◽  
Amirala Bakhshian Nik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Neural Tissue ◽  
Microfluidic Systems ◽  
Neural Tissue Engineering ◽  
Stem Cell Culture ◽  
Stem Cell Derivation

Overall process of stem cell derivation and isolation, as well as microfluidic stem cell culture and neural tissue engineering.

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