Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function

2014 ◽  
Vol 11 (2) ◽  
pp. 582-595 ◽  
Author(s):  
Serge Ostrovidov ◽  
Samad Ahadian ◽  
Javier Ramon-Azcon ◽  
Vahid Hosseini ◽  
Toshinori Fujie ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Pc12 Cells ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Tissue Formation ◽  
And Function

Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat

Nature Food ◽  
2020 ◽  
Vol 1 (4) ◽  
pp. 210-220 ◽  
Author(s):  
Tom Ben-Arye ◽  
Yulia Shandalov ◽  
Shahar Ben-Shaul ◽  
Shira Landau ◽  
Yedidya Zagury ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Soy Protein ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Protein Scaffolds ◽  
Bovine Skeletal Muscle

scholarly journals Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1781 ◽  
Author(s):  
Sara Politi ◽  
Felicia Carotenuto ◽  
Antonio Rinaldi ◽  
Paolo Di Nardo ◽  
Vittorio Manzari ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Muscle Tissue ◽  
Tissue Regeneration ◽  
Cell Function ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Electrospinning Process ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration

The development of smart and intelligent regenerative biomaterials for skeletal muscle tissue engineering is an ongoing challenge, owing to the requirement of achieving biomimetic systems able to communicate biological signals and thus promote optimal tissue regeneration. Electrospinning is a well-known technique to produce fibers that mimic the three dimensional microstructural arrangements, down to nanoscale and the properties of the extracellular matrix fibers. Natural and synthetic polymers are used in the electrospinning process; moreover, a blend of them provides composite materials that have demonstrated the potential advantage of supporting cell function and adhesion. Recently, the decellularized extracellular matrix (dECM), which is the noncellular component of tissue that retains relevant biological cues for cells, has been evaluated as a starting biomaterial to realize composite electrospun constructs. The properties of the electrospun systems can be further improved with innovative procedures of functionalization with biomolecules. Among the various approaches, great attention is devoted to the “click” concept in constructing a bioactive system, due to the modularity, orthogonality, and simplicity features of the “click” reactions. In this paper, we first provide an overview of current approaches that can be used to obtain biofunctional composite electrospun biomaterials. Finally, we propose a design of composite electrospun biomaterials suitable for skeletal muscle tissue regeneration.

scholarly journals Three-Dimensional Culture Model of Skeletal Muscle Tissue with Atrophy Induced by Dexamethasone

2017 ◽  
Vol 4 (4) ◽  
pp. 56 ◽  
Author(s):  
Kazunori Shimizu ◽  
Riho Genma ◽  
Yuuki Gotou ◽  
Sumire Nagasaka ◽  
Hiroyuki Honda
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Culture Model ◽  
Three Dimensional Culture

scholarly journals Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using GelatinMethacryloyl-Alginate Bioinks

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 679 ◽  
Author(s):  
Seyedmahmoud ◽  
Çelebi-Saltik ◽  
Barros ◽  
Nasiri ◽  
Banton ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
3D Bioprinting ◽  
Uv Crosslinking ◽  
Tissue Constructs ◽  
Muscle Tissues ◽  
Alginate Concentration

Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

scholarly journals Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

2020 ◽  
Vol 7 (3) ◽  
pp. 99
Author(s):  
Moon Sung Kang ◽  
Seok Hyun Lee ◽  
Won Jung Park ◽  
Ji Eun Lee ◽  
Bongju Kim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Interdisciplinary Studies ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration ◽  
Novel Strategy ◽  
Regenerate Tissue

Tissue engineering has recently emerged as a novel strategy for the regeneration of damaged skeletal muscle tissues due to its ability to regenerate tissue. However, tissue engineering is challenging due to the need for state-of-the-art interdisciplinary studies involving material science, biochemistry, and mechanical engineering. For this reason, electrospinning and three-dimensional (3D) printing methods have been widely studied because they can insert embedded muscle cells into an extracellular-matrix-mimicking microenvironment, which helps the growth of seeded or laden cells and cell signals by modulating cell–cell interaction and cell–matrix interaction. In this mini review, the recent research trends in scaffold fabrication for skeletal muscle tissue regeneration using advanced techniques, such as electrospinning and 3D bioprinting, are summarized. In conclusion, the further development of skeletal muscle tissue engineering techniques may provide innovative results with clinical potential for skeletal muscle regeneration.

scholarly journals Human Muscle Precursor Cells Overexpressing PGC-1α Enhance Early Skeletal Muscle Tissue Formation

2017 ◽  
Vol 26 (6) ◽  
pp. 1103-1114 ◽  
Author(s):  
Deana Haralampieva ◽  
Souzan Salemi ◽  
Ivana Dinulovic ◽  
Tullio Sulser ◽  
Simon M. Ametamey ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Precursor Cells ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Tissue Formation ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

scholarly journals Porcine skeletal muscle tissue fabrication for cultured meat production using three-dimensional bioprinting technology

2021 ◽  
Vol 1 (1) ◽  
pp. 88-97
Author(s):  
Yingying Li ◽  
Wenting Liu ◽  
Shilei Li ◽  
Mingyue Zhang ◽  
Feng Yang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Meat Production ◽  
Cultured Meat
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