scholarly journals Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

2020 ◽  
Vol 7 (3) ◽  
pp. 99
Author(s):  
Moon Sung Kang ◽  
Seok Hyun Lee ◽  
Won Jung Park ◽  
Ji Eun Lee ◽  
Bongju Kim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Interdisciplinary Studies ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration ◽  
Novel Strategy ◽  
Regenerate Tissue

Tissue engineering has recently emerged as a novel strategy for the regeneration of damaged skeletal muscle tissues due to its ability to regenerate tissue. However, tissue engineering is challenging due to the need for state-of-the-art interdisciplinary studies involving material science, biochemistry, and mechanical engineering. For this reason, electrospinning and three-dimensional (3D) printing methods have been widely studied because they can insert embedded muscle cells into an extracellular-matrix-mimicking microenvironment, which helps the growth of seeded or laden cells and cell signals by modulating cell–cell interaction and cell–matrix interaction. In this mini review, the recent research trends in scaffold fabrication for skeletal muscle tissue regeneration using advanced techniques, such as electrospinning and 3D bioprinting, are summarized. In conclusion, the further development of skeletal muscle tissue engineering techniques may provide innovative results with clinical potential for skeletal muscle regeneration.

scholarly journals Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1781 ◽  
Author(s):  
Sara Politi ◽  
Felicia Carotenuto ◽  
Antonio Rinaldi ◽  
Paolo Di Nardo ◽  
Vittorio Manzari ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Muscle Tissue ◽  
Tissue Regeneration ◽  
Cell Function ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Electrospinning Process ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Regeneration

The development of smart and intelligent regenerative biomaterials for skeletal muscle tissue engineering is an ongoing challenge, owing to the requirement of achieving biomimetic systems able to communicate biological signals and thus promote optimal tissue regeneration. Electrospinning is a well-known technique to produce fibers that mimic the three dimensional microstructural arrangements, down to nanoscale and the properties of the extracellular matrix fibers. Natural and synthetic polymers are used in the electrospinning process; moreover, a blend of them provides composite materials that have demonstrated the potential advantage of supporting cell function and adhesion. Recently, the decellularized extracellular matrix (dECM), which is the noncellular component of tissue that retains relevant biological cues for cells, has been evaluated as a starting biomaterial to realize composite electrospun constructs. The properties of the electrospun systems can be further improved with innovative procedures of functionalization with biomolecules. Among the various approaches, great attention is devoted to the “click” concept in constructing a bioactive system, due to the modularity, orthogonality, and simplicity features of the “click” reactions. In this paper, we first provide an overview of current approaches that can be used to obtain biofunctional composite electrospun biomaterials. Finally, we propose a design of composite electrospun biomaterials suitable for skeletal muscle tissue regeneration.

scholarly journals Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using GelatinMethacryloyl-Alginate Bioinks

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 679 ◽  
Author(s):  
Seyedmahmoud ◽  
Çelebi-Saltik ◽  
Barros ◽  
Nasiri ◽  
Banton ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
3D Bioprinting ◽  
Uv Crosslinking ◽  
Tissue Constructs ◽  
Muscle Tissues ◽  
Alginate Concentration

Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

Aligned and electrically conductive 3D collagen scaffolds for skeletal muscle tissue engineering

2021 ◽  
Author(s):  
Ivan M Basurto ◽  
Mark T Mora ◽  
Gregg M Gardner ◽  
George Christ ◽  
Steven R Caliari
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Muscle Fibers ◽  
Three Dimensional ◽  
Skeletal Muscle Tissue ◽  
Collagen Scaffolds ◽  
Electrically Conductive ◽  
Normal Movement ◽  
3D Collagen

Skeletal muscle is characterized by its three-dimensional (3D) anisotropic architecture composed of highly aligned and electrically-excitable muscle fibers that enable normal movement. Biomaterial-based tissue engineering approaches to repair skeletal muscle...

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Pigmented Silk Nanofibrous Composite for Skeletal Muscle Tissue Engineering

2016 ◽  
Vol 5 (10) ◽  
pp. 1222-1232 ◽  
Author(s):  
Shivaprasad Manchineella ◽  
Greeshma Thrivikraman ◽  
Khadija K. Khanum ◽  
Praveen C. Ramamurthy ◽  
Bikramjit Basu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue

scholarly journals Gelatin-genipin-based biomaterials for skeletal muscle tissue engineering

2018 ◽  
Vol 106 (8) ◽  
pp. 2763-2777 ◽  
Author(s):  
Francesca Gattazzo ◽  
Carmelo De Maria ◽  
Alessandro Rimessi ◽  
Silvia Donà ◽  
Paola Braghetta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue
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