Three-dimensional assembly of tissue-engineered cartilage constructs results in cartilaginous tissue formation without retainment of zonal characteristics

2013 ◽  
Vol 10 (4) ◽  
pp. 315-324 ◽  
Author(s):  
W. Schuurman ◽  
E. B. Harimulyo ◽  
D. Gawlitta ◽  
T. B. F. Woodfield ◽  
W. J. A. Dhert ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Tissue Formation ◽  
Cartilaginous Tissue ◽  
Engineered Cartilage

Tissue-Engineered Cartilage in Three-Dimensional Cultured Chondrocytes by Ultrasound Stimulation and Collagen Sponge as Scaffold

2009 ◽  
Author(s):  
Toshihiko Shiraishi ◽  
Ietomo Matsunaga ◽  
Shin Morishita ◽  
Ryohei Takeuchi ◽  
Tomoyuki Saito ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Three Dimensional ◽  
Collagen Sponge ◽  
Cartilage Tissue ◽  
Mechanical Environment ◽  
Ultrasound Stimulation ◽  
Scaffold Structure ◽  
Matrix Production ◽  
Engineered Cartilage ◽  
Cultured Chondrocytes

This paper describes the effects of ultrasound stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen sponges as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis). It has been shown that cell proliferation and matrix production can be facilitated by considering the mechanical environment of the cultured chondrocytes and the mechanical properties of the scaffold structure used in the culture and of the stimulation used.

scholarly journals Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3447-3461 ◽  
Author(s):  
Gaurav Agrawal ◽  
Aereas Aung ◽  
Shyni Varghese
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Tissue Formation ◽  
Microfluidic Platform ◽  
Muscle Tissues ◽  
Vitro Model

We introduce a microfluidic platform in which we culture three-dimensional skeletal muscle tissues, while evaluating tissue formation and toxin-induced muscle injury.

Collagen Expression in Tissue Engineered Cartilage of Aged Human Articular Chondrocytes in a Rotating Bioreactor

2003 ◽  
Vol 26 (4) ◽  
pp. 319-330 ◽  
Author(s):  
S. Marlovits ◽  
B. Tichy ◽  
M. Truppe ◽  
D. Gruber ◽  
W. Schlegel
Keyword(s):  
Electron Microscopy ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Three Dimensional ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Transmission Electron ◽  
Low Shear Stress ◽  
Engineered Cartilage

This study describes the culture and three-dimensional assembly of aged human articular chondrocytes under controlled oxygenation and low shear stress in a rotating-wall vessel. Chondrocytes cultured in monolayer were released and placed without any scaffold as a single cell suspension in a rotating bioreactor for 12 weeks. Samples were analyzed with immunohistochemistry, molecular biology and electron microscopy. During serial monolayer cultures chondrocytes dedifferentiated to a “fibroblast-like” structure and produced predominantly collagen type I. When these dedifferentiated cells were transferred to the rotating bioreactor, the cells showed a spontaneous aggregation and formation of solid tissue during the culture time. Expression of collagen type II and other components critical for the extracellular cartilage matrix could be detected. Transmission electron microscopy revealed a fine network of randomly distributed collagen fibrils. This rotating bioreactor proves to be a useful tool for providing an environment that enables dedifferentiated chondrocytes to redifferentiate and produce a cartilage-specific extracellular matrix.

scholarly journals Three-dimensional changes of noses after transplantation of implant-type tissue-engineered cartilage for secondary correction of cleft lip–nose patients

2017 ◽  
Vol 7 ◽  
pp. 72-79 ◽  
Author(s):  
Kazuto Hoshi ◽  
Yuko Fujihara ◽  
Hideto Saijo ◽  
Kumiko Kurabayashi ◽  
Hideyuki Suenaga ◽  
...  
Keyword(s):  
Cleft Lip ◽  
Three Dimensional ◽  
Dimensional Changes ◽  
Engineered Cartilage ◽  
Cleft Lip Nose

EFFECT OF BIOMECHANICAL CONDITIONING ON CARTILAGINOUS TISSUE FORMATION IN VITRO

2003 ◽  
Vol 85 ◽  
pp. 101-105 ◽  
Author(s):  
STEPHEN D. WALDMAN ◽  
CAROLINE G. SPITERI ◽  
MARC D. GRYNPAS ◽  
ROBERT M. PILLIAR ◽  
JASON HONG ◽  
...  
Keyword(s):  
Tissue Formation ◽  
Cartilaginous Tissue

Production of three-dimensional tissue-engineered cartilage through mutual fusion of chondrocyte pellets

2016 ◽  
Vol 45 (9) ◽  
pp. 1177-1185 ◽  
Author(s):  
K. Hoshi ◽  
Y. Fujihara ◽  
Y. Mori ◽  
Y. Asawa ◽  
S. Kanazawa ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Engineered Cartilage

scholarly journals 3D Nanoprinting Technologies for Tissue Engineering Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Jin Woo Lee
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Photon Absorption ◽  
Tissue Formation ◽  
Original Function ◽  
Cell Dynamics ◽  
Scaffold Fabrication ◽  
Two Photon Absorption ◽  
Two Photon

Tissue engineering recovers an original function of tissue by replacing the damaged part with a new tissue or organ regenerated using various engineering technologies. This technology uses a scaffold to support three-dimensional (3D) tissue formation. Conventional scaffold fabrication methods do not control the architecture, pore shape, porosity, or interconnectivity of the scaffold, so it has limited ability to stimulate cell growth and to generate new tissue. 3D printing technologies may overcome these disadvantages of traditional fabrication methods. These technologies use computers to assist in design and fabrication, so the 3D scaffolds can be fabricated as designed and standardized. Particularly, because nanofabrication technology based on two-photon absorption (2PA) and on controlled electrospinning can generate structures with submicron resolution, these methods have been evaluated in various areas of tissue engineering. Recent combinations of 3D nanoprinting technologies with methods from molecular biology and cell dynamics have suggested new possibilities for improved tissue regeneration. If the interaction between cells and scaffold system with biomolecules can be understood and controlled and if an optimal 3D environment for tissue regeneration can be realized, 3D nanoprinting will become an important tool in tissue engineering.

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