Adipogenesis using human adipose tissue-derived stromal cells combined with a collagen/gelatin sponge sustaining release of basic fibroblast growth factor

2012 ◽  
Vol 8 (12) ◽  
pp. 1000-1008 ◽  
Author(s):  
Ran Ito ◽  
Naoki Morimoto ◽  
Pham Hieu Liem ◽  
Yoko Nakamura ◽  
Katsuya Kawai ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Gelatin Sponge ◽  
Fibroblast Growth

Transplantation of basic fibroblast growth factor-pretreated adipose tissue-derived stromal cells enhances regression of liver fibrosis in mice

2009 ◽  
Vol 296 (2) ◽  
pp. G157-G167 ◽  
Author(s):  
Yoshihiro Kamada ◽  
Yuichi Yoshida ◽  
Yukiko Saji ◽  
Juichi Fukushima ◽  
Shinji Tamura ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Growth Factor ◽  
Liver Fibrosis ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Stromal Cells ◽  
Marker Genes ◽  
Specific Marker ◽  
Fibroblast Growth ◽  
Mice Liver

Adipose tissue-derived stromal cells (ADSC) potentially differentiate into various cell types similar to bone marrow-derived mesenchymal stromal cells (BMSC). Unlike BMSC, ADSC can be harvested easily and repeatedly. However, the advantages of ADSC for cell transplantation in liver disease remain unclear. To investigate this, we developed a novel culture system for ADSC, as well as effective methods for transplantation of ADSC into mice liver. ADSC were isolated from subcutaneous adipose tissues of male C57BL6/J mice and cultured on plastic dishes with or without basic fibroblast growth factor (bFGF). In the in vivo study, ADSC isolated from green fluorescent protein-transgenic mice were transplanted into carbon tetrachloride-injured C57BL6/J mice liver. bFGF-treated ADSC expressed several liver-specific marker genes and demonstrated liver-related functions such as albumin secretion, glycogen synthesis, urea production, and low-density lipoprotein uptake. Importantly, pretreatment of ADSC with bFGF for 1 wk enhanced the repopulation rate of ADSC in mice liver, attenuated liver fibrosis, and restored normal serum alanine aminotransferase and albumin levels. The results indicate that basic FGF facilitates transdifferentiation of ADSC into hepatic lineage cells in vitro and that transplantation of bFGF-pretreated ADSC reduced hepatic fibrosis in mice. ADSC are a potentially valuable source of cells for transplantation therapy.

scholarly journals Full-thickness skin reconstruction with basic fibroblast growth factor-impregnated collagen-gelatin sponge

2019 ◽  
Vol 11 ◽  
pp. 81-87 ◽  
Author(s):  
Hajime Matsumine ◽  
Hiroshi Fujimaki ◽  
Mika Takagi ◽  
Satoko Mori ◽  
Tomohiro Iwata ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Gelatin Sponge ◽  
Fibroblast Growth ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Reconstruction

Benign prostatic stromal cells are regulated by basic fibroblast growth factor and transforming growth factor-β1

1996 ◽  
Vol 151 (2) ◽  
pp. 315-322 ◽  
Author(s):  
A T Collins ◽  
E J Robinson ◽  
D E Neal
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Conditioned Medium ◽  
Basic Fibroblast Growth Factor ◽  
Stromal Cells ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Initial Increase ◽  
Fibroblast Growth ◽  
Mitogenic Effect

Abstract The current study was undertaken, using cultures of prostatic epithelial and stromal cells, to determine the functional interactions between androgens, basic fibroblast growth factor (FGF2) and transforming growth factor-β1 (TGFβ1) and their importance in maintaining stromal homeostasis. Treatment of stromal cells with TGFβ1 significantly increased intracellular FGF2 and FGF2 sequestered to the extracellular matrix. FGF2 was also detected in stromal conditioned medium (SCM), but at levels 70-fold less than found in cell lysates. TGFβ1 (0·1 ng/ml) treatment caused an initial increase of 86% in secreted FGF2 levels, but high concentrations of TGFβ1 (5 ng/ml) decreased FGF2 levels by 38%, relative to the untreated control. Further studies showed that epithelial conditioned medium (ECM), androgen-treated, stromal conditioned medium (ASCM), but not SCM were mitogenic for stromal cells. Both ECM and ASCM caused a threefold increase in DNA synthesis. FGF2 may be the mediator of these interactions, since the mitogenic effect of both ECM and ASCM was significantly reduced by the addition of anti-FGF2 neutralising antibody. We hypothesise that the lack of response of stromal cells to SCM is due to TGFβ1 blocking the mitogenic effect of FGF2. Thus down-regulation of TGFβ1 synthesis, by androgens, results in stromal proliferation by ASCM. Journal of Endocrinology (1996) 151, 315–322

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