Ellagic acid embryoprotection in vitro: Distribution and effects on DNA adduct formation

Teratology ◽  
1993 ◽  
Vol 47 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Anthony A. Frank ◽  
John M. Collier ◽  
Carol S. Forsyth ◽  
Wenguang Zeng ◽  
Gary D. Stoner
Keyword(s):  
Ellagic Acid ◽  
Adduct Formation ◽  
Dna Adduct

DNA adduct formation by secondary metabolites of cyclopenta[cd]pyrene in vitro

1999 ◽  
Vol 136 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Ching-Hung Hsu ◽  
Paul L. Skipper ◽  
Steven R. Tannenbaum
Keyword(s):  
Secondary Metabolites ◽  
Adduct Formation ◽  
Dna Adduct

DNA adduct formation by O-phenylphenol metabolite in vivo and in vitro

1992 ◽  
Vol 13 (8) ◽  
pp. 1469-1473 ◽  
Author(s):  
Keiko Ushiyama ◽  
Fumiko Nagai ◽  
Atsuko Nakagawa ◽  
Itsu Kano
Keyword(s):  
Adduct Formation ◽  
Dna Adduct

Nitrite Curing of Chicken, Pork, and Beef Inhibits Oxidation but Does Not AffectN-Nitroso Compound (NOC)-Specific DNA Adduct Formation during in Vitro Digestion

2014 ◽  
Vol 62 (8) ◽  
pp. 1980-1988 ◽  
Author(s):  
Thomas Van Hecke ◽  
Julie Vanden Bussche ◽  
Lynn Vanhaecke ◽  
Els Vossen ◽  
John Van Camp ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Nitroso Compound

O6-carboxymethylguanine DNA adduct formation and lipid peroxidation upon in vitro gastrointestinal digestion of haem-rich meat

2014 ◽  
Vol 58 (9) ◽  
pp. 1883-1896 ◽  
Author(s):  
Julie Vanden Bussche ◽  
Lieselot Y. Hemeryck ◽  
Thomas Van Hecke ◽  
Gunter G. C. Kuhnle ◽  
Frank Pasmans ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Gastrointestinal Digestion ◽  
In Vitro Gastrointestinal Digestion

scholarly journals Effect of 2-acetylaminofluorene and its genotoxic metabolites on DNA adduct formation and DNA damage in 3D reconstructed human skin tissue models

Mutagenesis ◽  
2019 ◽  
Author(s):  
Thomas R Downs ◽  
Volker M Arlt ◽  
Brenda C Barnett ◽  
Ryan Posgai ◽  
Stefan Pfuhler
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Skin ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Strong Increase ◽  
Multiple Exposures ◽  
Multiple Treatments ◽  
Reconstructed Human Skin

Abstract In vitro genotoxicity assays utilising human skin models are becoming important tools for the safety assessment of chemicals whose primary exposure is via the dermal route. In order to explore metabolic competency and inducibility of CYP450 activating enzymes, 3D reconstructed human skin tissues were topically treated with 2-acetylaminofluorene (2-AAF) and its genotoxic metabolites, N-hydroxy-2-acetylaminofluorene (N-OH-2-AAF) and N-hydroxy-2-aminofluorene (N-OH-2-AF), which primarily cause DNA damage by forming DNA adducts. 2-AAF did not increase DNA damage measured in the reconstructed skin micronucleus (RSMN) assay when administered in multiple applications at 24 h intervals but was detected in the skin comet assay in the presence of the DNA polymerase inhibitor aphidicolin (APC). Similarly, no increase was found with N-OH-2-AAF in the RSMN assay after multiple treatments whereas a single 3 h exposure to N-OH-2-AAF caused a large dose-related increase in the skin comet assay. A significant increase in the RSMN assay was only obtained with the highly reactive N-OH-2-AF metabolite after multiple treatments over 72 h, whereas N-OH-2-AF caused a strong increase after a single 3 h exposure in the skin comet assay. In support of these results, DNA adduct formation, measured by the 32P-postlabelling assay, was examined. Adduct levels after 2-AAF treatment for 3 h were minimal but increased >10-fold after multiple exposures over 48 h, suggesting that enzyme(s) that metabolise 2-AAF are induced in the skin models. As expected, a single 3 h exposure to N-OH-2-AAF and N-OH-2-AF resulted in adduct levels that were at least 10-fold greater than those after multiple exposures to 2-AAF despite ~100-fold lower tested concentrations. Our results demonstrate that DNA damage caused by 2-AAF metabolites is more efficiently detected in the skin comet assay than the RSMN assay and after multiple exposures and enzyme induction, 2-AAF-induced DNA damage can be detected in the APC-modified comet assay.

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