scholarly journals (2839) Proposal to conserve the name Triticum caninum ( Elymus caninus ) ( Poaceae ) with a conserved type

Taxon ◽  
2021 ◽  
Vol 70 (5) ◽  
pp. 1138-1139
Author(s):  
Santiago Andrés‐Sánchez ◽  
Víctor Lucía ◽  
M. Montserrat Martínez‐Ortega ◽  
Enrique Rico
Keyword(s):  
Elymus Caninus

Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 420-431 ◽  
Author(s):  
Gen-Lou Sun ◽  
Oscar Díaz ◽  
Björn Salomon ◽  
Roland von Bothmer
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Microsatellite Markers ◽  
Correlation Coefficients ◽  
Geographic Isolation ◽  
Elymus Caninus ◽  
Distance Matrices ◽  
High Level ◽  
Mantel’S Test ◽  
Similarity Matrices

Genetic diversity of 33 Elymus caninus accessions was investigated using isozyme, RAPD, and microsatellite markers. The three assays differed in the amount of polymorphism detected. Microsatellites detected the highest polymorphism. Six microsatellite primer pairs generated a total of 74 polymorphic bands (alleles), with an average of 15.7 bands per primer pair. Three genetic similarity matrices were estimated based on band presence or absence. Genetic diversity trees (dendrograms) were derived from each marker technique, and compared using Mantel's test. The correlation coefficients were 0.204, 0.267, and 0.164 between isozyme and RAPD distance matrices, RAPD and microsatellite distance matrices, and between isozyme and microsatellite distance matrices, respectively. The three methodologies gave differing views of the amount of variation present but all showed a high level of genetic variation in E. caninus. The following points may be drawn from this study whether based on RAPD, microsatellite, or isozyme data: (i) The Icelandic populations are consistently revealed by the three dendrograms. The congruence of the discrimination of this accession group by RAPD, microsatellite, and isozyme markers suggests that geographic isolation strongly influenced the evolution of the populations; (ii) The degree of genetic variation within accessions was notably great; and (iii) The DNA-based markers will be the more useful ones in detecting genetic diversity in closely related accessions. In addition, a dendrogram, which took into account all fragments produced by isozymes, RAPDs, and microsatellites, reflected better the relationships than did dendrograms based on only one type of marker.Key words: Elymus caninus, genetic diversity, isozymes, RAPDs, microsatellites.

Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae)

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Marian Ørgaard ◽  
Kesara Anamthawat-Jónsson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic Dna ◽  
Genomic In Situ Hybridization ◽  
Pseudoroegneria Spicata ◽  
Genome Constitution ◽  
Elytrigia Repens ◽  
Elymus Caninus ◽  
Elymus Species

The genome constitution of Icelandic Elymus caninus, E. alaskanus, and Elytrigia repens was examined by fluorescence in situ hybridization using genomic DNA and selected cloned sequences as probes. Genomic in situ hybridization (GISH) of Hordeum brachyantherum ssp. californicum (diploid, H genome) probe confirmed the presence of an H genome in the two tetraploid Elymus species and identified its presence in the hexaploid Elytrigia repens. The H chromosomes were painted uniformly except for some chromosomes of Elytrigia repens which showed extended unlabelled pericentromeric and subterminal regions. A mixture of genomic DNA from H. marinum ssp. marinum (diploid,Xa genome) and H. murinum ssp. leporinum (tetraploid,Xu genome) did not hybridize to chromosomes of the Elymus species or Elytrigia repens, confirming that these genomes were different from the H genome. The St genomic probe from Pseudoroegneria spicata (diploid) did not discriminate between the genomes of the Elymus species, whereas it produced dispersed and spotty hybridization signals most likely on the two St genomes of Elytrigia repens. Chromosomes of the two genera Elymus and Elytrigia showed different patterns of hybridization with clones pTa71 and pAes41, while clones pTa1 and pSc119.2 hybridized only to Elytrigia chromosomes. Based on FISH with these genomic and cloned probes, the two Elymus species are genomically similar, but they are evidently different from Elytrigia repens. Therefore the genomes of Icelandic Elymus caninus and E. alaskanus remain as StH, whereas the genomes of Elytrigia repens are proposed as XXH.Key words: Elymus, Elytrigia, H genome, St genome, in situ hybridization.

Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 420-431 ◽  
Author(s):  
Gen-Lou Sun ◽  
Oscar Díaz ◽  
Björn Salomon ◽  
Roland von Bothmer
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Elymus Caninus

scholarly journals Specific genetic markers for wheat, spelt, and four wild relatives: comparison of isozymes, RAPDs, and wheat microsatellites

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 610-621 ◽  
Author(s):  
Roberto Guadagnuolo ◽  
Dessislava Savova Bianchi ◽  
François Felber
Keyword(s):  
Genetic Variation ◽  
Wild Species ◽  
Wild Relatives ◽  
Species Differentiation ◽  
Large Set ◽  
Strongly Correlated ◽  
Aegilops Cylindrica ◽  
Elymus Caninus ◽  
Set Up ◽  
Mantel’S Test

Three types of markers—isozymes, RAPDs (random amplified polymorphic DNAs), and wheat microsatellites—were tested on wheat, spelt, and four wild wheat relatives (Aegilops cylindrica, Elymus caninus, Hordeum marinum, and Agropyron junceum). The aim was to evaluate their capability to provide specific markers for differentiation of the cultivated and wild species. The markers were set up for subsequent detection of hybrids and introgression of wheat DNA into wild relatives. All markers allowed differentiation of the cultivated from the wild species. Wheat microsatellites were not amplified in all the wild relatives, whereas RAPDs and isozymes exhibited polymorphism for all species. The dendrograms obtained with RAPD and isozyme data separated Swiss wheat cultivars from those collected in Austria and England, while no difference was found between Swiss spelt and wheat. RAPD data provided a weak discrimination between English and Austrian E. caninus. The microsatellite-based dendrogram discriminated populations of Ae. cylindrica, but no clear separation of H. marinum from E. caninus was revealed. The similarity matrices based on the three different sets of data were strongly correlated. The highest value was recorded between the matrices based on RAPDs and isozymes (Mantel's test, r = 0.93). Correlations between the similarity matrix based on microsatellites and matrices based on RAPDs and isozymes were lower: 0.74 and 0.68, respectively. While microsatellites are very useful for comparisons of closely related accessions, they are less suitable for studies involving less-related taxa. Isozymes provide interesting markers for species differentiation, but their use seems less appropriate for studies of within-species genetic variation. RAPDs can produce a large set of markers, which can be used for the evaluation of both between- and within-species genetic variation, more rapidly and easily than isozymes and microsatellites.Key words: Triticeae, isozymes, RAPDs, microsatellites, polymorphism.

Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers

Genome ◽  
1997 ◽  
Vol 40 (6) ◽  
pp. 806-814 ◽  
Author(s):  
Gen-Lou Sun ◽  
Björn Salomon ◽  
Roland von Bothmer
Keyword(s):  
Microsatellite Markers ◽  
Rapd Markers ◽  
Species Group ◽  
Population Diversity ◽  
Morphological Classification ◽  
Genome Species ◽  
Species Cluster ◽  
Elymus Caninus ◽  
Elymus Species

An analysis of Amplification fragment polymorphism of DNA from 27 accessions of 19 tetraploid Elymus species was carried out using 18 wheat microsatellite (WMS) primer pairs and 10 decamer primers. Ten WMS primer pairs produced multiple polymorphism on all accessions tested. Two independent phenograms, one based on WMS-PCR and one on RAPDs, separated the 19 tetraploid species into two main groups, viz., the SH genome species group and the SY genome species group. The results coincide with the genomic classification of these species and hence support previous studies showing that Elymus is not a monophyletic genus. The assays indicated that accessions within a species cluster together, which concurs with the morphological classification. Interspecific and intraspecific polymorphisms were detected by the WMS-PCR and RAPD analyses. Variation was observed among accessions of Elymus caninus. The WMS-PCR detected a much higher level of polymorphism than the RAPD analysis. WMSs seem to be more efficient markers than RAPD markers for studying the population diversity of Elymus species. The potential of cross-species amplification of microsatellite markers as an additional source for genetic analysis and applications in Elymus is discussed in the context of these results.Key words: Elymus, wheat, RAPD, phenetics, microsatellites.

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