HC-HA/PTX3 Purified From Amniotic Membrane Promotes BMP Signaling in Limbal Niche Cells to Maintain Quiescence of Limbal Epithelial Progenitor/Stem Cells

Stem Cells ◽  
2015 ◽  
Vol 33 (11) ◽  
pp. 3341-3355 ◽  
Author(s):  
Szu-Yu Chen ◽  
Bo Han ◽  
Ying-Ting Zhu ◽  
Megha Mahabole ◽  
Jie Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Bmp Signaling

scholarly journals Periostin as a Biomarker of the Amniotic Membrane

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Mariya P. Dobreva ◽  
Larissa Lhoest ◽  
Paulo N. G. Pereira ◽  
Lieve Umans ◽  
Susana M. Chuva de Sousa Lopes ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Target Genes ◽  
Genetic Model ◽  
Bmp Signaling ◽  
Marker Genes ◽  
Transcriptional Signature ◽  
Mrna Enrichment ◽  
Extraembryonic Membranes ◽  
Extraembryonic Tissues

Tracing the precise developmental origin of amnion and amnion-derived stem cells is still challenging and depends chiefly on analyzing powerful genetic model amniotes like mouse. Profound understanding of the fundamental differences in amnion development in both the disc-shaped primate and human embryo and the cup-shaped mouse embryo is pivotal in particular when sampling amniotic membrane from nonprimate species for isolating candidate amniotic stem cells. The availability of molecular marker genes that are specifically expressed in the amniotic membrane and not in other extraembryonic membranes would be instrumental to validate unequivocally the starting material under investigation. So far such amniotic markers have not been reported. We postulated that bone morphogenetic protein (BMP) target genes are putative amniotic membrane markers mainly because deficiency in one of several components of the BMP signaling cascade in mice has been documented to result in defective development of the early amnion. Comparative gene expression analysis of acknowledged target genes for BMP in different extraembryonic tissues, combined within situhybridization, identifiedPeriostin (Postn)mRNA enrichment in amnion throughout gestation. In addition, we identify and propose a combination of markers as transcriptional signature for the different extraembryonic tissues in mouse.

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

scholarly journals Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation

2015 ◽  
Vol 35 (10) ◽  
pp. 1700-1711 ◽  
Author(s):  
Fenfang Chen ◽  
Xia Lin ◽  
Pinglong Xu ◽  
Zhengmao Zhang ◽  
Yanzhen Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Nuclear Export ◽  
Stem Cell Differentiation ◽  
Bmp Signaling ◽  
Dependent Manner ◽  
Mesenchymal Stem Cell Differentiation

Bone morphogenetic proteins (BMPs) play vital roles in regulating stem cell maintenance and differentiation. BMPs can induce osteogenesis and inhibit myogenesis of mesenchymal stem cells. Canonical BMP signaling is stringently controlled through reversible phosphorylation and nucleocytoplasmic shuttling of Smad1, Smad5, and Smad8 (Smad1/5/8). However, how the nuclear export of Smad1/5/8 is regulated remains unclear. Here we report that the Ran-binding protein RanBP3L acts as a nuclear export factor for Smad1/5/8. RanBP3L directly recognizes dephosphorylated Smad1/5/8 and mediates their nuclear export in a Ran-dependent manner. Increased expression of RanBP3L blocks BMP-induced osteogenesis of mouse bone marrow-derived mesenchymal stem cells and promotes myogenic induction of C2C12 mouse myoblasts, whereas depletion of RanBP3L expression enhances BMP-dependent stem cell differentiation activity and transcriptional responses. In conclusion, our results demonstrate that RanBP3L, as a nuclear exporter for BMP-specific Smads, plays a critical role in terminating BMP signaling and regulating mesenchymal stem cell differentiation.

(+)-Cholesten-3-one induces differentiation of neural stem cells into dopaminergic neurons through BMP signaling

2010 ◽  
Vol 68 (3) ◽  
pp. 176-184 ◽  
Author(s):  
Dong-Feng Chen ◽  
Ling-Jie Meng ◽  
Shao-Hui Du ◽  
Hai-Ling Zhang ◽  
Hui Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dopaminergic Neurons ◽  
Bmp Signaling

scholarly journals Dual Inhibition of Activin/Nodal/TGF-βand BMP Signaling Pathways by SB431542 and Dorsomorphin Induces Neuronal Differentiation of Human Adipose Derived Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Vedavathi Madhu ◽  
Abhijit S. Dighe ◽  
Quanjun Cui ◽  
D. Nicole Deal
Keyword(s):  
Stem Cells ◽  
Nervous System ◽  
Neuronal Differentiation ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
Bmp Signaling ◽  
Specific Marker ◽  
Adipose Derived Stem Cells ◽  
Neuronal Marker ◽  
Dual Inhibition

Damage to the nervous system can cause devastating diseases or musculoskeletal dysfunctions and transplantation of progenitor stem cells can be an excellent treatment option in this regard. Preclinical studies demonstrate that untreated stem cells, unlike stem cells activated to differentiate into neuronal lineage, do not survive in the neuronal tissues. Conventional methods of inducing neuronal differentiation of stem cells are complex and expensive. We therefore sought to determine if a simple, one-step, and cost effective method, previously reported to induce neuronal differentiation of embryonic stem cells and induced-pluripotent stem cells, can be applied to adult stem cells. Indeed, dual inhibition of activin/nodal/TGF-βand BMP pathways using SB431542 and dorsomorphin, respectively, induced neuronal differentiation of human adipose derived stem cells (hADSCs) as evidenced by formation of neurite extensions, protein expression of neuron-specific gamma enolase, and mRNA expression of neuron-specific transcription factors Sox1 and Pax6 and matured neuronal marker NF200. This process correlated with enhanced phosphorylation of p38, Erk1/2, PI3K, and Akt1/3. Additionally,in vitrosubcutaneous implants of SB431542 and dorsomorphin treated hADSCs displayed significantly higher expression of active-axonal-growth-specific marker GAP43. Our data offers novel insights into cell-based therapies for the nervous system repair.

scholarly journals SIRT6 promotes osteogenic differentiation of mesenchymal stem cells through BMP signaling

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ping Zhang ◽  
Yunsong Liu ◽  
Yuejun Wang ◽  
Min Zhang ◽  
Longwei Lv ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bmp Signaling

scholarly journals BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice

Development ◽  
2017 ◽  
Vol 144 (14) ◽  
pp. 2560-2569 ◽  
Author(s):  
Jifan Feng ◽  
Junjun Jing ◽  
Jingyuan Li ◽  
Hu Zhao ◽  
Vasu Punj ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcriptional Network ◽  
Bmp Signaling
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