scholarly journals Histone Deacetylase Inhibition with Valproic Acid Downregulates Osteocalcin Gene Expression in Human Dental Pulp Stem Cells and Osteoblasts: Evidence for HDAC2 Involvement

Stem Cells ◽  
2014 ◽  
Vol 32 (1) ◽  
pp. 279-289 ◽  
Author(s):  
Francesca Paino ◽  
Marcella La Noce ◽  
Virginia Tirino ◽  
Pasqualina Naddeo ◽  
Vincenzo Desiderio ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Valproic Acid ◽  
Histone Deacetylase ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Histone Deacetylase Inhibition ◽  
Osteocalcin Gene ◽  
Human Dental Pulp

scholarly journals The Histone Deacetylase Inhibitor (MS-275) Promotes Differentiation of Human Dental Pulp Stem Cells into Odontoblast-Like Cells Independent of the MAPK Signaling System

2020 ◽  
Vol 21 (16) ◽  
pp. 5771
Author(s):  
Eun-Cheol Lee ◽  
Yu-Mi Kim ◽  
Han-Moi Lim ◽  
Ga-Eun Ki ◽  
Young-Kwon Seo
Keyword(s):  
Stem Cells ◽  
Histone Deacetylase ◽  
Dental Pulp ◽  
Epigenetic Modification ◽  
Cell Types ◽  
Mapk Signaling ◽  
Dental Pulp Stem Cells ◽  
Signaling System ◽  
Dental Tissue ◽  
Human Dental Pulp

The role of dental pulp stem cells (DPSCs) in dental tissue regeneration is gaining attention because DPSCs can differentiate into odontoblasts and other specialized cell types. Epigenetic modification has been found to play an important role in cell differentiation and regulation, among which histone deacetylase (HDAC) is involved in suppressing genes by removing histone acetyl groups. The use of HDAC inhibitor to control this is increasing and has been widely studied by many researchers. This study aimed to induce differentiation by causing epigenetic changes in odontoblast-related genes and the MAPK signaling pathway in human dental pulp stem cells. Western blot and immunofluorescence staining showed increased expression of DMP-1, ALP, DSPP, and RUNX2 compared to the control. However, activation of the MAPK signaling system was similar to but slightly different from the expression of odontoblast-related proteins. After 3 days, as shown by MTT and LDH assays, proliferation decreased overall, but cytotoxicity decreased at only a specific concentration. We confirmed that there was no change in mRNA expression of caspase 3 or 9 using real-time PCR. In addition, flow cytometry analysis confirmed that differentiation occurred due to the decrease in the expression of the CD73 and CD146. Although overall proliferation was reduced due to the G2/M inhibition of the cell cycle, the expression of BCL-2 protected the cells from cell death. Overall, cell proliferation decreased in response to MS-275, but it did not induce cytotoxicity in 5 nM and 10 nM concentration and induces differentiation into odontoblast-like cells.

549-P: Transplantation of Human Dental Pulp Stem Cells Ameliorates Diabetic Polyneuropathy via Increasing Angiogenic and Neurotrophic Gene Expression in the Transplanted Muscles

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 549-P
Author(s):  
MASAKI HATA ◽  
MAIKO OMI ◽  
NOBUHISA NAKAMURA ◽  
MEGUMI MIYABE ◽  
MIZUHO ITO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Dental Pulp ◽  
Diabetic Polyneuropathy ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elanagai Rathinam ◽  
Srinath Govindarajan ◽  
Sivaprakash Rajasekharan ◽  
Heidi Declercq ◽  
Dirk Elewaut ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Dental Pulp ◽  
Maximum Amplitude ◽  
Tricalcium Silicate ◽  
Dental Pulp Stem Cells ◽  
Alizarin Red ◽  
Mineralization Potential ◽  
Intracellular Ca ◽  
Human Dental Pulp

AbstractCalcium (Ca2+) signalling plays an indispensable role in dental pulp and dentin regeneration, but the Ca2+ responses of human dental pulp stem cells (hDPSCs) stimulated with tricalcium silicate-based (TCS-based) dental biomaterials remains largely unexplored. The objective of the present study was to identify and correlate extracellular Ca2+ concentration, intracellular Ca2+ dynamics, pH, cytotoxicity, gene expression and mineralization ability of human dental pulp stem cells (hDPSCs) stimulated with two different TCS-based biomaterials: Biodentine and ProRoot white MTA. The hDPSCs were exposed to the biomaterials, brought in contact with the overlaying medium, with subsequent measurements of extracellular Ca2+ and pH, and intracellular Ca2+ changes. Messenger RNA expression (BGLAP, TGF-β, MMP1 and BMP2), cytotoxicity (MTT and TUNEL) and mineralization potential (Alizarin red and Von Kossa staining) were then evaluated. Biodentine released significantly more Ca2+ in the α-MEM medium than ProRoot WMTA but this had no cytotoxic impact on hDPSCs. The larger Biodentine-linked Ca2+ release resulted in altered intracellular Ca2+ dynamics, which attained a higher maximum amplitude, faster rise time and increased area under the curve of the Ca2+ changes compared to ProRoot WMTA. Experiments with intracellular Ca2+ chelation, demonstrated that the biomaterial-triggered Ca2+ dynamics affected stem cell-related gene expression, cellular differentiation and mineralization potential. In conclusion, biomaterial-specific Ca2+ dynamics in hDPSCs determine differentiation and mineralization outcomes, with increased Ca2+ dynamics enhancing mineralization.

scholarly journals Characterisation of proliferation, differentiation potential, and gene expression among clonal cultures of human dental pulp cells

2019 ◽  
Author(s):  
Tomoko Kobayashi ◽  
Daisuke Torii ◽  
Takanori Iwata ◽  
Yuichi Izumi ◽  
Masanori Nasu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Marker Genes ◽  
Differentiation Potential ◽  
Candidate Marker ◽  
Cell Clones ◽  
Human Dental Pulp

Abstract Background: Mesenchymal stem cells are a highly promising source of cells for regeneration therapy because of their multilineage differentiation potential. However, distinct markers for mesenchymal stem cells are not well-established. To identify new candidate marker genes for multipotent human dental pulp stem cells, we analysed the characteristics and gene expression profiles of cell clones obtained from a single dental pulp specimen.Results: Fifty colony-forming single cell-derived clones were isolated from a single dental pulp specimen. These clones varied in their proliferation abilities and surface marker (STRO-1 and CD146) expression patterns, as well as their odontogenic, adipogenic, and chondrogenic differentiation potentials. Four clones maintained their original differentiation potentials during long-term culture. Gene expression profile analysis of five representative clones identified 1227 genes that were related to multipotency. Ninety of these 1227 genes overlapped with genes reportedly involved in ‘stemness or differentiation’. Based on the predicted locations of expressed protein products and large changes in expression levels, 14 of the 90 genes were selected as candidate dental pulp stem cell markers, particularly in relation to their multipotency characteristics.Conclusions: This characterisation of cell clones obtained from a single specimen of human dental pulp provided information regarding new candidate marker genes for multipotent dental pulp stem cells, which could facilitate efficient analysis or enrichment of multipotent stem cells.

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