scholarly journals Histone Deacetylase 1 Deficiency Impairs Differentiation and Electrophysiological Properties of Cardiomyocytes Derived from Induced Pluripotent Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (11) ◽  
pp. 2412-2422 ◽  
Author(s):  
Eneda Hoxha ◽  
Erin Lambers ◽  
Hehuang Xie ◽  
Alexandre De Andrade ◽  
Prasanna Krishnamurthy ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Pluripotent Cells ◽  
Electrophysiological Properties ◽  
Histone Deacetylase 1 ◽  
Induced Pluripotent Cells ◽  
Induced Pluripotent

scholarly journals Obtaining Consent for Future Research with Induced Pluripotent Cells: Opportunities and Challenges

PLoS Biology ◽  
2009 ◽  
Vol 7 (2) ◽  
pp. e1000042 ◽  
Author(s):  
Katriina Aalto-Setälä ◽  
Bruce R Conklin ◽  
Bernard Lo
Keyword(s):  
Future Research ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Induced Pluripotent

scholarly journals Questioned validity of Gene Expression Dysregulated Domains in Down's Syndrome

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 269 ◽  
Author(s):  
Long H. Do ◽  
William C. Mobley ◽  
Nishant Singhal
Keyword(s):  
Gene Expression ◽  
Monozygotic Twins ◽  
Recent Analysis ◽  
Ts65dn Mouse ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Independent Analysis ◽  
Human Ipscs ◽  
Rnaseq Data ◽  
Induced Pluripotent

Recently, in studies examining fibroblasts obtained from the tissues of one set of monozygotic twins (i.e. fetuses derived from the same egg) discordant for trisomy 21 (Down syndrome; DS), Letourneau et al., reported the presence of a defined pattern of dysregulation within specific genomic domains they referred to as Gene Expression Dysregulated Domains (GEDDs). GEDDs were described as alternating segments of increased or decreased gene expression affecting all chromosomes. Strikingly, GEDDs in fibroblasts were largely conserved in induced pluripotent cells (iPSCs) generated from the twin’s fibroblasts as well as in fibroblasts from the Ts65Dn mouse model of DS. Our recent analysis failed to find GEDDs. We reexamined the human iPSCs RNAseq data from Letourneau et al., and data from this same research group published earlier examining iPSCs from the same monozygotic twins. An independent analysis of RNAseq data from Ts65Dn fibroblasts also failed to confirm presence of GEDDs. Our analysis questions the validity of GEDDs in DS.

Abstract 340: From Stem Cells to Cardiomyocytes: HDAC1 Induces Cardiovascular Differentiation by Regulating SOX17-BMP2 Expression in Early Development.

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Eneda Hoxha ◽  
Erin Lambers ◽  
Veronica Ramirez ◽  
Prasanna Krishnamurthy ◽  
Suresh Verma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Mechanisms ◽  
Es Cells ◽  
Ips Cells ◽  
Full Potential ◽  
Pluripotent Cells ◽  
Differentiation Potential ◽  
Histone Deacetylase 1 ◽  
Specific Differentiation ◽  
Induced Pluripotent

Cardiomyocytes derived from embryonic and induced pluripotent stem cells (ES/iPS) provide an excellent source for cell replacement therapies following myocardial ischemia. However, some of the obstacles in the realization of the full potential of iPS/ES cells arise from incomplete and poorly understood molecular mechanisms and epigenetic modifications that govern their cardiovascular specific differentiation. We identified Histone Deacetylase 1 (HDAC1) as a crucial regulator in early differentiation of mES and iPS cells. We propose a novel pathway in which HDAC1 regulates cardiovascular differentiation by regulating SOX17 which in turn regulates BMP2 signaling in differentiating pluripotent cells. Utilizing stable HDAC1 knock-down (HDAC1-KD) cell lines, we report an essential role for HDAC1 in deacetylating regulatory regions of pluripotency-associated genes during early cardiovascular differentiation. HDAC1-KD cells show severely repressed cardiomyocyte differentiation potential. We propose a novel HDAC1-BMP2-SOX17 dependent pathway through which deacetylation of pluripotency associated genes leads to their suppression and allows for early cardiovascular-associated genes to be expressed and differentiation to occur. Furthermore, we show that HDAC1 affects DNA methylation both during pluripotency and differentiation and plays a crucial, non-redundant role in cardiovascular specific differentiation and cardiomyocyte maturation. Our data elucidates important differences between ES and iPS HDAC1-KD cells that affect their ability to differentiate into cardiovascular lineages. As varying levels of chromatin modifying enzymes are likely to exist in patient derived iPS cells, understanding the molecular circuitry of these enzymes in ES and iPS cells is critical for their potential therapeutic applications in regenerative medicine. Further research in the molecular mechanisms involved in this process will greatly aid our understanding of the epigenetic circuitry of pluripotency and differentiation in pluripotent cells.

scholarly journals A defined culture method enabling the establishment of ring sideroblasts from induced pluripotent cells of X-linked sideroblastic anemia

Haematologica ◽  
2018 ◽  
Vol 103 (5) ◽  
pp. e188-e191 ◽  
Author(s):  
Shunsuke Hatta ◽  
Tohru Fujiwara ◽  
Takako Yamamoto ◽  
Kei Saito ◽  
Mayumi Kamata ◽  
...  
Keyword(s):  
Culture Method ◽  
Pluripotent Cells ◽  
Sideroblastic Anemia ◽  
Induced Pluripotent Cells ◽  
Ring Sideroblasts ◽  
Induced Pluripotent ◽  
Defined Culture

CRISPR/Cas9 Nuclease-Mediated Gene Knock-In in Bovine-Induced Pluripotent Cells

2015 ◽  
Vol 24 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Young Tae Heo ◽  
Xiaoyuan Quan ◽  
Yong Nan Xu ◽  
Soonbong Baek ◽  
Hwan Choi ◽  
...  
Keyword(s):  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Cas9 Nuclease ◽  
Induced Pluripotent

CC Chemokine Ligand 2 and Leukemia Inhibitory Factor Cooperatively Promote Pluripotency in Mouse Induced Pluripotent Cells

Stem Cells ◽  
2011 ◽  
Vol 29 (8) ◽  
pp. 1196-1205 ◽  
Author(s):  
Yuki Hasegawa ◽  
Naoko Takahashi ◽  
Alistair R. R. Forrest ◽  
Jay W. Shin ◽  
Yohei Kinoshita ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Inhibitory Factor ◽  
Pluripotent Cells ◽  
Cc Chemokine ◽  
Induced Pluripotent Cells ◽  
Chemokine Ligand ◽  
Induced Pluripotent ◽  
Cc Chemokine Ligand 2 ◽  
Chemokine Ligand 2
Sign in / Sign up

Export Citation Format

Share Document