scholarly journals Thermal Stability of Fibroblast Growth Factor Protein Is a Determinant Factor in Regulating Self-Renewal, Differentiation, and Reprogramming in Human Pluripotent Stem Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (4) ◽  
pp. 623-630 ◽  
Author(s):  
Guokai Chen ◽  
Daniel R. Gulbranson ◽  
Pengzhi Yu ◽  
Zhonggang Hou ◽  
James A. Thomson
Keyword(s):  
Thermal Stability ◽  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Fibroblast Growth ◽  
Self Renewal ◽  
Determinant Factor ◽  
Thermal Stability Of

Human Caesarean scar-derived feeder cells: a novel feeder cell type for culturing human pluripotent stem cells without exogenous basic fibroblast growth factor supplementation

2020 ◽  
Vol 32 (9) ◽  
pp. 822
Author(s):  
Wipawee Pavarajarn ◽  
Ruttachuk Rungsiwiwut ◽  
Pranee Numchaisrika ◽  
Pramuan Virutamasen ◽  
Kamthorn Pruksananonda
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Fibroblast Growth ◽  
Feeder Cell ◽  
Feeder Cells ◽  
Cell Type ◽  
Caesarean Scar

In a feeder-dependent culture system of human pluripotent stem cells (hPSCs), coculture with mouse embryonic fibroblasts may limit the clinical use of hPSCs. The aim of this study was to determine the feasibility of using human Caesarean scar fibroblasts (HSFs) as feeder cells for the culture of hPSCs. HSFs were isolated and characterised and cocultured with hPSCs, and the pluripotency, differentiation ability and karyotypic stability of hPSCs were determined. Inactivated HSFs expressed genes (including inhibin subunit beta A (INHBA), bone morphogenetic protein 4 (BMP4), fibroblast growth factor 2 (FGF2), transforming growth factor-β1 (TGFB1), collagen alpha-1(I) (COL1A1) and fibronectin-1 (FN1) that have been implicated in the maintenance of hPSC pluripotency. When HSFs were used as feeder cells, the pluripotency and karyotypic stability of hPSC lines did not change after prolonged coculture. Interestingly, exogenous FGF2 could be omitted from the culture medium when HSFs were used as feeder cells for hESCs but not hiPSCs. hESCs cocultured with HSF feeder cells in medium without FGF2 supplementation maintained their pluripotency (as confirmed by the expression of pluripotency markers and genes), differentiated invitro into embryonic germ layers and maintained their normal karyotype. The present study demonstrates that HSFs are a novel feeder cell type for culturing hPSCs and that supplementation of exogenous FGF2 is not necessary for the Chula2.hES line.

290 GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM BOVINE FETAL FIBROBLAST CELLS BY DEFINED FACTORS

2011 ◽  
Vol 23 (1) ◽  
pp. 243 ◽  
Author(s):  
H. G. Cao ◽  
Y. Liu ◽  
H. Q. Yin ◽  
X. P. Sun ◽  
Y. S. Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pluripotent Stem Cells ◽  
Leukemia Inhibitory Factor ◽  
Fibroblast Cells ◽  
Fibroblast Growth ◽  
Fetal Fibroblast ◽  
Induced Pluripotent

Induced pluripotent stem cells (iPS) have broad potential applications in drug screening, regenerative medicine, and basic biology, and using iPS as starting materials that have identical properties to embryonic stem cell would probably revolutionize the production of gene-targeted livestock. However, iPS in livestock is still lacking except for in pigs. Instructed by Yamanaka’s idea, in the current study we attempted to generate bovine iPS from fetal fibroblast cells (bFF; from a fetus 2.5 months old after gestation) by using 4 defined transcriptional factors: Oct-4, Sox2, Klf4, and c-Myc. A lentivirus haboring the 4 factors acted as a vehicle to transfect the bFF. One bFF cell line was infected for at least 3 replicates. After transfection, the treated bFF were then cultured in DMEM supplemented with 4 ng mL–1 of basic fibroblast growth factor and 1000 IU mL–1 of leukemia inhibitory factor at 37.5°C, 5% CO2, on the mouse embryonic feeder cells pretreatd with mitomycin C. From Day 16 after the onset of induction, morphology of a few typical spindle-like bFF gradually changed into round, ball-like cells and grew into colonies. Afterward, when these colonies were harvested and subcultured in stem cell medium supplemented with 1000 IU mL–1 of leukemia inhibitory factor and 4 ng mL–1 of basic fibroblast growth factor, new colonies with clear-cut, round edge emerged, and the cells in the colonies had increased nuclear:cytoplasm ratio, kept normal karyotype up to 10 passages, and were alkaline phosphatase staining positive. We also found that the cells exhibited part of the stem cell markers, as evidenced by being Nanog, SSEA1 positive but SSEA3 and TRA-60 negative. Moreover, embryoid bodies could be formed in vitro, and terotoma formation after injection into nude mice also displayed 3 layers. Taken together, we found that 4 lentivirus-mediated, defined transcriptional factors could successfully induce bFF into iPS-like cells. H. G. Cao and Y. Liu contributed equally to this work. The corresponding authors are Y. H. Zhang and X. R. Zhang. This work was supported by NSFC (30800784/c120103, 30700574), 973 (2009CB941004).

scholarly journals Fibroblast Growth Factor Signaling Is Essential for Self-renewal of Dental Epithelial Stem Cells

2013 ◽  
Vol 288 (40) ◽  
pp. 28952-28961 ◽  
Author(s):  
Julia Yu Fong Chang ◽  
Cong Wang ◽  
Junchen Liu ◽  
Yanqing Huang ◽  
Chengliu Jin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Growth Factor Signaling ◽  
Fibroblast Growth ◽  
Epithelial Stem Cells ◽  
Fibroblast Growth Factor Signaling ◽  
Self Renewal

Effects of pH and Polyanions on the Thermal Stability of Fibroblast Growth Factor 20

2007 ◽  
Vol 4 (2) ◽  
pp. 232-240 ◽  
Author(s):  
Haihong Fan ◽  
Samadhi N. Vitharana ◽  
Tracy Chen ◽  
Donald O'Keefe ◽  
C. Russell Middaugh
Keyword(s):  
Thermal Stability ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Effects Of Ph ◽  
Thermal Stability Of ◽  
Factor 20
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