scholarly journals Three‐Dimensional Visualization of Subcellular Dynamics of Cancer Cell Destruction on Therapeutic Nanodrug Treatment

2021 ◽  
pp. 2000145
Author(s):  
Sreejith Raveendran ◽  
Anindito Sen ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Cancer Cell ◽  
Three Dimensional ◽  
Cell Destruction

scholarly journals Three‐Dimensional Visualization of Subcellular Dynamics of Cancer Cell Destruction on Therapeutic Nanodrug Treatment

2021 ◽  
Vol 2 (7) ◽  
pp. 2170018
Author(s):  
Sreejith Raveendran ◽  
Anindito Sen ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Cancer Cell ◽  
Three Dimensional ◽  
Cell Destruction

Three‐dimensional cancer cell culture in high‐yield multiscale scaffolds by shear spinning

2018 ◽  
Vol 35 (2) ◽  
pp. e2750 ◽  
Author(s):  
Ahmed A. Ahmed ◽  
CJ Luo ◽  
Sandra Perez‐Garrido ◽  
Connor R. Browse ◽  
Christopher Thrasivoulou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Three Dimensional ◽  
High Yield ◽  
Shear Spinning

scholarly journals Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171417 ◽  
Author(s):  
María Anguiano ◽  
Carlos Castilla ◽  
Martin Maška ◽  
Cristina Ederra ◽  
Rafael Peláez ◽  
...  
Keyword(s):  
Image Analysis ◽  
Cell Migration ◽  
Cancer Cell ◽  
Three Dimensional ◽  
Cancer Cell Migration

Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions

Lab on a Chip ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 1378-1387 ◽  
Author(s):  
Manami Sugimoto ◽  
Yoichi Kitagawa ◽  
Masumi Yamada ◽  
Yuya Yajima ◽  
Rie Utoh ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Quantitative Evaluation ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Cancer Cell Invasion ◽  
New System

A new system for quantitatively evaluating cancer cell invasion in a three-dimensional environment was developed using composite hydrogel microfibers having a micropassage.

Cancer Cell Image Analysis and Visualization

2012 ◽  
pp. 233-243
Author(s):  
Tae-Yun Kim ◽  
Hae-Gil Hwang ◽  
Heung-Kook Choi
Keyword(s):  
Image Analysis ◽  
Feature Extraction ◽  
Case Studies ◽  
Cancer Cell ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Dimensional Image ◽  
The Past ◽  
Cell Image ◽  
Cell Image Analysis

We review computerized cancer cell image analysis and visualization research over the past 30 years. Image acquisition, feature extraction, classification, and visualization from two-dimensional to three-dimensional image algorithms are introduced with case studies of bladder, prostate, breast, and renal carcinomas.

scholarly journals Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis

2011 ◽  
Vol 22 (3) ◽  
pp. 327-341 ◽  
Author(s):  
Nicholas O. Deakin ◽  
Christopher E. Turner
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Morphology ◽  
Molecular Mechanisms ◽  
Adhesion Formation ◽  
Three Dimensional ◽  
Rho Kinase ◽  
Adaptor Protein ◽  
Metastatic Tumor

Individual metastatic tumor cells exhibit two interconvertible modes of cell motility during tissue invasion that are classified as either mesenchymal or amoeboid. The molecular mechanisms by which invasive breast cancer cells regulate this migratory plasticity have yet to be fully elucidated. Herein we show that the focal adhesion adaptor protein, paxillin, and the closely related Hic-5 have distinct and unique roles in the regulation of breast cancer cell lung metastasis by modulating cell morphology and cell invasion through three-dimensional extracellular matrices (3D ECMs). Cells depleted of paxillin by RNA interference displayed a highly elongated mesenchymal morphology, whereas Hic-5 knockdown induced an amoeboid phenotype with both cell populations exhibiting reduced plasticity, migration persistence, and velocity through 3D ECM environments. In evaluating associated signaling pathways, we determined that Rac1 activity was increased in cells devoid of paxillin whereas Hic-5 silencing resulted in elevated RhoA activity and associated Rho kinase–induced nonmuscle myosin II activity. Hic-5 was essential for adhesion formation in 3D ECMs, and analysis of adhesion dynamics and lifetime identified paxillin as a key regulator of 3D adhesion assembly, stabilization, and disassembly.

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