Optical Imaging and High‐Accuracy Quantification of Intracellular Iron Contents

Small ◽  
2020 ◽  
pp. 2005474
Author(s):  
Dewen Ye ◽  
Mingxi Li ◽  
Yuanyuan Xie ◽  
Bo Chen ◽  
Yuexia Han ◽  
...  
Small ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 2170005
Author(s):  
Dewen Ye ◽  
Mingxi Li ◽  
Yuanyuan Xie ◽  
Bo Chen ◽  
Yuexia Han ◽  
...  

2015 ◽  
Vol 5 (5) ◽  
pp. 1422-1427 ◽  
Author(s):  
Ricky B. Dunbar ◽  
Augustin Barbe ◽  
Christopher J. Fell

2016 ◽  
Vol 45 (12) ◽  
pp. 1211002
Author(s):  
李航 LI Hang ◽  
颜昌翔 YAN Chang-xiang ◽  
邵建兵 SHAO Jian-bing ◽  
王小朋 WANG Xiao-peng

Author(s):  
M. Nishigaki ◽  
S. Katagiri ◽  
H. Kimura ◽  
B. Tadano

The high voltage electron microscope has many advantageous features in comparison with the ordinary electron microscope. They are a higher penetrating efficiency of the electron, low chromatic aberration, high accuracy of the selected area diffraction and so on. Thus, the high voltage electron microscope becomes an indispensable instrument for the metallurgical, polymer and biological specimen studies. The application of the instrument involves today not only basic research but routine survey in the various fields. Particularly for the latter purpose, the performance, maintenance and reliability of the microscope should be same as those of commercial ones. The authors completed a 500 kV electron microscope in 1964 and a 1,000 kV one in 1966 taking these points into consideration. The construction of our 1,000 kV electron microscope is described below.


Author(s):  
M. Ashraf ◽  
F. Thompson ◽  
S. Miki ◽  
P. Srivastava

Iron is believed to play an important role in the pathogenesis of ischemic injury. However, the sources of intracellular iron in myocytes are not yet defined. In this study we have attempted to localize iron at various cellular sites of the cardiac tissue with the ferrocyanide technique.Rat hearts were excised under ether anesthesia. They were fixed with coronary perfusion with 3% buffered glutaraldehyde made in 0.1 M cacodylate buffer pH 7.3. Sections, 60 μm in thickness, were cut on a vibratome and were incubated in the medium containing 500 mg of potassium ferrocyanide in 49.5 ml H2O and 0.5 ml concentrated HC1 for 30 minutes at room temperature. Following rinses in the buffer, tissues were dehydrated in ethanol and embedded in Spurr medium.The examination of thin sections revealed intense staining or reaction product in peroxisomes (Fig. 1).


2004 ◽  
Vol 171 (4S) ◽  
pp. 289-289
Author(s):  
Gabri van der Pluijm ◽  
Antoinette Wetterwald ◽  
George N. Thalmann ◽  
Guus Lycklama A. Nijeholt ◽  
Rob Pelger ◽  
...  

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