scholarly journals Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events

Small ◽  
2020 ◽  
Vol 16 (52) ◽  
pp. 2004148
Author(s):  
Fenni Zhang ◽  
Jiapei Jiang ◽  
Michelle McBride ◽  
Yunze Yang ◽  
Manni Mo ◽  
...  
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Optical Tracking ◽  
Urine Samples

Interference Disturbance Analysis Enables Single-Cell Level Growth and Mobility Characterization for Rapid Antimicrobial Susceptibility Testing

Nano Letters ◽  
2018 ◽  
Vol 19 (2) ◽  
pp. 643-651 ◽  
Author(s):  
David Volbers ◽  
Valentin K. Stierle ◽  
Konstantin J. Ditzel ◽  
Julian Aschauer ◽  
Joachim O. Rädler ◽  
...  
Keyword(s):  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Single Cell Level ◽  
Cell Level ◽  
Disturbance Analysis

scholarly journals Single Cell Antimicrobial Susceptibility Testing by Confined Microchannels and Electrokinetic Loading

2013 ◽  
Vol 85 (8) ◽  
pp. 3971-3976 ◽  
Author(s):  
Yi Lu ◽  
Jian Gao ◽  
Donna D. Zhang ◽  
Vincent Gau ◽  
Joseph C. Liao ◽  
...  
Keyword(s):  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing

601 A VERSATILE BIOSENSOR PLATFORM FOR RAPID ANTIMICROBIAL SUSCEPTIBILITY TESTING DIRECTLY FROM URINE SAMPLES

2010 ◽  
Vol 183 (4S) ◽  
Author(s):  
Kathleen E. Mach ◽  
Ruchika Mohan ◽  
Mei-Chiung Shih ◽  
Ellen Jo Baron ◽  
Vincent Gau ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Urine Samples

scholarly journals A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 288
Author(s):  
Britney Forsyth ◽  
Peter Torab ◽  
Jyong-Huei Lee ◽  
Tyler Malcom ◽  
Tza-Huei Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Rapid Determination ◽  
Bloodstream Infections ◽  
Antimicrobial Susceptibility Testing ◽  
Resistant Bacteria ◽  
Bloodborne Pathogens ◽  
The Matrix ◽  
Single Cell Trapping

Bloodstream infections are a significant cause of morbidity and mortality worldwide. The rapid initiation of effective antibiotic treatment is critical for patients with bloodstream infections. However, the diagnosis of bloodborne pathogens is largely complicated by the matrix effect of blood and the lengthy blood tube culture procedure. Here we report a culture-free workflow for the rapid isolation and enrichment of bacterial pathogens from whole blood for single-cell antimicrobial susceptibility testing (AST). A dextran sedimentation step reduces the concentration of blood cells by 4 orders of magnitude in 20–30 min while maintaining the effective concentration of bacteria in the sample. Red blood cell depletion facilitates the downstream centrifugation-based enrichment step at a sepsis-relevant bacteria concentration. The workflow is compatible with common antibiotic-resistant bacteria and does not influence the minimum inhibitory concentrations. By applying a microfluidic single-cell trapping device, we demonstrate the workflow for the rapid determination of bacterial infection and antimicrobial susceptibility testing at the single-cell level. The entire workflow from blood to categorical AST result can be completed in less than two hours.

scholarly journals Antimicrobial Susceptibility Pattern of Urinary Pathogens in a Tertiary Care Hospital

2021 ◽  
Vol 10 (12) ◽  
pp. 878-882
Author(s):  
Akshatha N ◽  
Anjana Gopi ◽  
Supriya Christopher ◽  
Mangala G.K.
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Urine Culture ◽  
Antimicrobial Susceptibility Testing ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern

BACKGROUND Urinary tract Infection (UTI) are the most common clinical conditions in general practice and gynaecological department. Men and women of all age groups are affected by UTI, but its overall prevalence is higher in women. The major causative agents are Escherichia coli and other Enterobacteriaceae. In majority of the cases, empirically treatment is started with antimicrobials before the urine culture and sensitivity reports become available. This has led to an increase in antibiotic resistance in urinary pathogens. Hence, the present study was undertaken to determine the antimicrobial susceptibility pattern of uropathogens to commonly used antimicrobials in treating UTIs. METHODS A total of 200 urine samples were obtained from patients with signs and symptoms suggestive of UTIs attending Bapuji and Chigateri General Hospital which are attached to Jagadguru Jayadeva Murugarajendra Medical College (JJMMC), Davangere for a period of 3 months that is from May 2016 to July 2016. Urine samples were processed within 2 hours of reaching the laboratory. Semi quantitative urine culture was done. Isolation and identification were done by performing standard biochemical tests and the antimicrobial susceptibility testing was done using the standard disc diffusion method by Kirby-Bauer technique. RESULTS There were 170 urinary isolates from 200 samples. Children less than 10 years were more affected 54 (27 %). Female patients were more, 119 (59.5 %) compared to males 81 (40.5 %). Escherichia coli was the most predominant isolate, 71 (41.8 %) followed by Enterococcus species 22 (1.9 %). Organisms were resistant to commonly used antibiotics i.e., cotrimoxazole, ciprofloxacin, norfloxacin and amoxicillin. Both gram negative and gram-positive isolates were sensitive to amikacin and nitrofurantoin. CONCLUSIONS In present study Escherichia coli is the predominant pathogen. Uncomplicated UTIs can be empirically treated by nitrofurantoin. Occurrence of treatment failure with commonly used antimicrobials is more often in Indian setting. Hence, antimicrobial susceptibility testing must be employed routinely. KEY WORDS UTIs, Uropathogens, E. coli, Antimicrobial Susceptibility Pattern

scholarly journals Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing

2019 ◽  
Vol 116 (21) ◽  
pp. 10270-10279 ◽  
Author(s):  
Hui Li ◽  
Peter Torab ◽  
Kathleen E. Mach ◽  
Christine Surrette ◽  
Matthew R. England ◽  
...  
Keyword(s):  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Bacterial Pathogens ◽  
Microfluidic System ◽  
Antimicrobial Susceptibility Testing ◽  
Microbiological Analysis ◽  
Single Cell Level ◽  
Cell Level

Infectious diseases caused by bacterial pathogens remain one of the most common causes of morbidity and mortality worldwide. Rapid microbiological analysis is required for prompt treatment of bacterial infections and to facilitate antibiotic stewardship. This study reports an adaptable microfluidic system for rapid pathogen classification and antimicrobial susceptibility testing (AST) at the single-cell level. By incorporating tunable microfluidic valves along with real-time optical detection, bacteria can be trapped and classified according to their physical shape and size for pathogen classification. By monitoring their growth in the presence of antibiotics at the single-cell level, antimicrobial susceptibility of the bacteria can be determined in as little as 30 minutes compared with days required for standard procedures. The microfluidic system is able to detect bacterial pathogens in urine, blood cultures, and whole blood and can analyze polymicrobial samples. We pilot a study of 25 clinical urine samples to demonstrate the clinical applicability of the microfluidic system. The platform demonstrated a sensitivity of 100% and specificity of 83.33% for pathogen classification and achieved 100% concordance for AST.

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