Assembly of Functional Three-Dimensional Neuronal Networks on a Microchip

Small ◽  
2014 ◽  
Vol 10 (13) ◽  
pp. 2530-2536 ◽  
Author(s):  
Zhuo Huang ◽  
Yi Sun ◽  
Wenwen Liu ◽  
Wei Zhang ◽  
Wenfu Zheng ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Three Dimensional

Neuronal Networks: Assembly of Functional Three-Dimensional Neuronal Networks on a Microchip (Small 13/2014)

Small ◽  
2014 ◽  
Vol 10 (13) ◽  
pp. 2736-2736
Author(s):  
Zhuo Huang ◽  
Yi Sun ◽  
Wenwen Liu ◽  
Wei Zhang ◽  
Wenfu Zheng ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Three Dimensional

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain

2007 ◽  
Vol 4 (4) ◽  
pp. 331-336 ◽  
Author(s):  
Hans-Ulrich Dodt ◽  
Ulrich Leischner ◽  
Anja Schierloh ◽  
Nina Jährling ◽  
Christoph Peter Mauch ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Neuronal Networks ◽  
Three Dimensional

scholarly journals Three dimensional microenvironments on multi-electrode arrays produce neuronal networks that function like the brain

2018 ◽  
Vol 12 ◽  
Author(s):  
Justin Bourke ◽  
Anita Quigley ◽  
Cathal O'Connell ◽  
Jeremy Crook ◽  
Gordon Wallace ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Three Dimensional ◽  
Electrode Arrays ◽  
The Brain

scholarly journals 3D arrays of microcages by two-photon lithography for spatial organization of living cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 875-884 ◽  
Author(s):  
Florian Larramendy ◽  
Shotaro Yoshida ◽  
Daniela Maier ◽  
Zoltan Fekete ◽  
Shoji Takeuchi ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Living Cells ◽  
Artificial Organs ◽  
Two Photon ◽  
3D Network

This paper addresses a nanoengineering approach to create a fully three-dimensional (3D) network of living cells, providing an advanced solution to in vitro studies on either neuronal networks or artificial organs.

Ultrasoft Alginate Hydrogels Support Long-Term Three-Dimensional Functional Neuronal Networks

2015 ◽  
Vol 21 (15-16) ◽  
pp. 2177-2185 ◽  
Author(s):  
Gemma Palazzolo ◽  
Nicolas Broguiere ◽  
Orlando Cenciarelli ◽  
Harald Dermutz ◽  
Marcy Zenobi-Wong
Keyword(s):  
Neuronal Networks ◽  
Three Dimensional ◽  
Alginate Hydrogels

Rapid generation of functional engineered 3D human neuronal assemblies: network dynamics evaluated by Micro-Electrodes Arrays

2021 ◽  
Author(s):  
Lorenzo Muzzi ◽  
Donatella Di Lisa ◽  
Pietro Arnaldi ◽  
Davide Aprile ◽  
Laura Pastorino ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Three Dimensional ◽  
Electrode Array ◽  
Good Control ◽  
Neuronal Population ◽  
Point Of View ◽  
Network Dynamic ◽  
Electrode Arrays ◽  
Neuronal Assemblies

Abstract Objective: In this work we propose a method for producing engineered human derived three-dimensional neuronal assemblies coupled to Micro-Electrode Array (MEA) substrates for studying the electrophysiological activity of such networks. Approach: We used biocompatible chitosan microbeads as scaffold to build 3D networks and to ensure nutrients-medium exchange from the core of the structure to the external environment. We used excitatory neurons derived from human-induced Pluripotent Stem Cells (hiPSCs) co-cultured with astrocytes. By adapting the well-established NgN2 differentiation protocol, we obtained 3D engineered networks with good control over cell density, volume and cell composition. We coupled the 3D neuronal networks to 60-channel Micro Electrode Arrays (MEAs) to evaluate and monitor the functional activity of the neuronal population. In parallel, we generated two-dimensional neuronal networks to compare the results of the two models. Main results: 3D cultures were healthy and functional up to 42 Days In Vitro (DIVs). From the structural point of view, the hiPSC derived neurons were able to adhere to chitosan microbeads and to form a stable 3D assembly thanks to the connections among cells. From a functional point of view, neuronal networks showed spontaneous activity after a couple of weeks. We monitored the functional electrophysiological behavior up to 6 weeks and we compared the network dynamic with 2D models. Significance: We presented for the first time a method to generate 3D engineered cultures with human-derived neurons coupled to MEAs, overcoming some of the limitations related to 2D and 3D neuronal networks and thus increasing the therapeutic target potential of these models for biomedical applications.

scholarly journals The brain of Cataglyphis ants: neuronal organization and visual projections

2020 ◽  
Author(s):  
Jens Habenstein ◽  
Emad Amini ◽  
Kornelia Grübel ◽  
Basil el Jundi ◽  
Wolfgang Rössler
Keyword(s):  
Visual Information ◽  
Neuronal Networks ◽  
Three Dimensional ◽  
Visual Features ◽  
Insect Brain ◽  
Neuronal Organization ◽  
Distance Information ◽  
Fiber Tracts ◽  
Central Brain ◽  
The Brain

AbstractCataglyphis ants are known for their outstanding navigational abilities. They return to their inconspicuous nest after far-reaching foraging trips using path integration, and whenever available, learn and memorize visual features of panoramic sceneries. To achieve this, the ants combine directional visual information from celestial cues and panoramic scenes with distance information from an intrinsic odometer. The largely vision-based navigation in Cataglyphis requires sophisticated neuronal networks to process the broad repertoire of visual stimuli. Although Cataglyphis ants have been subject to many neuroethological studies, little is known about the general neuronal organization of their central brain and the visual pathways beyond major circuits. Here, we provide a comprehensive, three-dimensional neuronal map of synapse-rich neuropils in the brain of Cataglyphis nodus including major connecting fiber systems. In addition, we examined neuronal tracts underlying the processing of visual information in more detail. This study revealed a total of 33 brain neuropils and 30 neuronal fiber tracts including six distinct tracts between the optic lobes and the cerebrum. We also discuss the importance of comparative studies on insect brain architecture for a profound understanding of neuronal networks and their function.

scholarly journals Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds

2017 ◽  
Vol 13 (4) ◽  
pp. 1563-1573 ◽  
Author(s):  
Albin Jakobsson ◽  
Maximilian Ottosson ◽  
Marina Castro Zalis ◽  
David O'Carroll ◽  
Ulrica Englund Johansson ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Electrospun Fiber ◽  
Three Dimensional ◽  
Low Density ◽  
Fiber Scaffolds

Human neural stem cell-derived cultures in three-dimensional substrates form spontaneously functional neuronal networks

2015 ◽  
Vol 11 (4) ◽  
pp. 1022-1033 ◽  
Author(s):  
Imogen Smith ◽  
Vasco Silveirinha ◽  
Jason L. Stein ◽  
Luis de la Torre-Ubieta ◽  
Jonathan A. Farrimond ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Neuronal Networks ◽  
Three Dimensional ◽  
Human Neural Stem Cell
Sign in / Sign up

Export Citation Format

Share Document