High Throughput-Per-Footprint Inertial Focusing

Small ◽  
2013 ◽  
Vol 9 (16) ◽  
pp. 2764-2773 ◽  
Author(s):  
Ata Tuna Ciftlik ◽  
Maxime Ettori ◽  
Martin A. M. Gijs
Keyword(s):  
High Throughput ◽  
Inertial Focusing

High-throughput particle focusing and separation in split-recombination channel

2022 ◽  
Vol 32 (2) ◽  
pp. 025007
Author(s):  
Shuang Chen ◽  
Zongqian Shi ◽  
Jiajia Sun ◽  
Shenli Jia ◽  
Mingjie Zhong ◽  
...  
Keyword(s):  
High Throughput ◽  
Lift Force ◽  
Curvature Radius ◽  
Straight Channel ◽  
Geometrical Structures ◽  
Inertial Focusing ◽  
Particle Focusing ◽  
Drag Forces ◽  
Recombination Channel ◽  
Flow Drag

Abstract Inertial microfluidic has been widely applied to manipulate particles or bio-sample based on the inertial lift force and Dean Vortices. This technology provides significant advantages over conventional technologies, including simple structure, high throughput and freedom from an external field. Among many inertial microfluidic systems, the straight microchannel is commonly used to produce inertial focusing, which is a phenomenon that particles or cells are aligned and separated based on their size under the influence of inertial lift force. Besides the inertial lift force, flow drag forces induced by the geometrical structures of microchannel can also affect particle focusing. Herein, a split-recombination microchannel, consisting of curved and straight channels, is proposed to focus and separate particles at high flow rate. As compared with the straight channel, the particle focusing in the split-recombination channel is greatly improved, which results from the combined effects of the inertial lift force, the curvature-induced Dean drag force and the structure of split and recombination. Moreover, the distribution of different-sized particles in designed microchannel is investigated. The results indicate that the proposed microchannel not only enhances the particle focusing but also enables the separation of different-sized particles with high throughput. Finally, it is discovered that the larger length of straight channel and curvature radius of curved channel can result in a more efficient particle separation. Another important feature of designed split-recombination microchannel is that it can be arranged in parallel to handle large-volume samples, holding great potential in lab-on-a-chip applications.

Microfluidic Impedance Cytometer with Inertial Focusing and Liquid Electrodes for High-Throughput Cell Counting and Discrimination

2017 ◽  
Vol 89 (5) ◽  
pp. 3154-3161 ◽  
Author(s):  
Wenlai Tang ◽  
Dezhi Tang ◽  
Zhonghua Ni ◽  
Nan Xiang ◽  
Hong Yi
Keyword(s):  
High Throughput ◽  
Cell Counting ◽  
Inertial Focusing ◽  
Liquid Electrodes

scholarly journals Microfluidics: High Throughput-Per-Footprint Inertial Focusing (Small 16/2013)

Small ◽  
2013 ◽  
Vol 9 (16) ◽  
pp. 2828-2828
Author(s):  
Ata Tuna Ciftlik ◽  
Maxime Ettori ◽  
Martin A. M. Gijs
Keyword(s):  
High Throughput ◽  
Inertial Focusing

High Throughput Cell-Free Extraction of Plasma by an Integrated Microfluidic Device Combining Inertial Focusing and Membrane

2017 ◽  
Vol 139 (5) ◽  
Author(s):  
Jun Zhang ◽  
Sheng Yan ◽  
Dan Yuan ◽  
Gursel Alici ◽  
Nam-Trung Nguyen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Microfluidic Device ◽  
High Throughput ◽  
Membrane Filtration ◽  
Membrane Filter ◽  
Whole Body ◽  
Inertial Focusing ◽  
High Efficient ◽  
Plasma Extraction

Plasma is a host of numerous analytes such as proteins, metabolites, circulating nucleic acids (CNAs), and pathogens, and it contains massive information about the functioning of the whole body, which is of great importance for the clinical diagnosis. Plasma needs to be completely cell-free for effective detection of these analytes. The key process of plasma extraction is to eliminate the contamination from blood cells. Centrifugation, a golden standard method for blood separation, is generally lab-intensive, time consuming, and even dangerous to some extent, and needs to be operated by well-trained staffs. Membrane filtration can filter cells very effectively according to its pore size, but it is prone to clogging by dense particle concentration and suffers from limited capacity of filtration. Frequent rinse is lab-intensive and undesirable. In this work, we proposed and fabricated an integrated microfluidic device that combined particle inertial focusing and membrane filter for high efficient blood plasma separation. The integrated microfluidic device was evaluated by the diluted (×1/10, ×1/20) whole blood, and the quality of the extracted blood plasma was measured and compared with that from the standard centrifugation. We found that the quality of the extracted blood plasma from the proposed device can be equivalent to that from the standard centrifugation. This study demonstrates a significant progress toward the practical application of inertial microfluidics with membrane filter for high-throughput and highly efficient blood plasma extraction.

scholarly journals High pressure inertial focusing for separating and concentrating bacteria at high throughput

2017 ◽  
Vol 27 (8) ◽  
pp. 084001 ◽  
Author(s):  
J Cruz ◽  
S Hooshmand Zadeh ◽  
T Graells ◽  
M Andersson ◽  
J Malmström ◽  
...  
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Inertial Focusing

scholarly journals Hybrid microfluidic sorting of rare cells based on high throughput inertial focusing and high accuracy acoustic manipulation

RSC Advances ◽  
2019 ◽  
Vol 9 (53) ◽  
pp. 31186-31195 ◽  
Author(s):  
Yinning Zhou ◽  
Zhichao Ma ◽  
Ye Ai
Keyword(s):  
High Throughput ◽  
High Accuracy ◽  
Cell Populations ◽  
Inertial Focusing ◽  
Fold Enrichment ◽  
Rare Cells ◽  
Microfluidic Sorting

The first example of integration of sized-based inertial sorting and surface biomarker-based acoustic sorting to achieve >2500-fold enrichment of rare cell populations.

scholarly journals Staged inertial microfluidic focusing for complex fluid enrichment

RSC Advances ◽  
2015 ◽  
Vol 5 (66) ◽  
pp. 53857-53864 ◽  
Author(s):  
Amy E. Reece ◽  
Kaja Kaastrup ◽  
Hadley D. Sikes ◽  
John Oakey
Keyword(s):  
High Throughput ◽  
High Yield ◽  
Inertial Focusing ◽  
Cellular Assays ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
Complex Fluid

A staged microfluidic inertial focusing device capable of high-yield, high-throughput complex fluid enrichment has been developed for integrated microfluidic cellular assays and biological micro total analysis systems.

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