Rhodamine‐Based Fluorescence ‘Turn‐On’ Chemosensor: Detection of Fe 3+ Ion in Aqueous Medium and MCF‐7 Live Cells

2021 ◽  
Vol 6 (9) ◽  
pp. 2373-2378
Author(s):  
Pooja S. Badekar ◽  
Garima C. N. Thakur ◽  
Mokshada E. Varma ◽  
Niraj S. Ghatpande ◽  
Prasad P. Kulkarni ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Live Cells ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Mcf 7

A naphthalimide based PET probe with Fe3+ selective detection ability: theoretical and experimental study

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6352-6356 ◽  
Author(s):  
Narendra Reddy Chereddy ◽  
M. V. Niladri Raju ◽  
Peethani Nagaraju ◽  
Venkat Raghavan Krishnaswamy ◽  
Purna Sai Korrapati ◽  
...  
Keyword(s):  
Experimental Study ◽  
Live Cells ◽  
Selective Detection ◽  
Aqueous Samples ◽  
Detection Ability ◽  
Turn On ◽  
Fluorescence Turn On

A naphthalimide based Fe3+ selective fluorescence ‘turn-on’ probe that operates based on a PET mechanism has been synthesized, and its application in the detection of Fe3+ ions in aqueous samples and in live cells is explored.

A highly selective and sensitive fluorescence “turn-on” probe for Ag+ in aqueous solution and live cells

2012 ◽  
Vol 41 (24) ◽  
pp. 7212 ◽  
Author(s):  
Lin Xu ◽  
Yufang Xu ◽  
Weiping Zhu ◽  
Chunmei Yang ◽  
Le Han ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Live Cells ◽  
Turn On ◽  
Fluorescence Turn On

Fluorescence Turn on Sensor for Sulfate Ion in Aqueous Medium Using Tripodal-Cu2+ Ensemble

2013 ◽  
Vol 24 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Md. Najbul Hoque ◽  
Arghya Basu ◽  
Gopal Das
Keyword(s):  
Aqueous Medium ◽  
Sulfate Ion ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 420
Author(s):  
Gyu Seong Yeom ◽  
In-ho Song ◽  
Shrikant Dashrath Warkad ◽  
Pramod B. Shinde ◽  
Taewoon Kim ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Damage ◽  
Human Urine ◽  
Live Cells ◽  
Turn On ◽  
Highly Sensitive ◽  
Bio Imaging ◽  
Fluorescence Turn On ◽  
Fluorescence Spectrometer ◽  
Excellent Selectivity

The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (ABIA) along with the design and development of a portable fluorescence spectrometer device (CysDDev) for detecting cysteine in simulated human urine. ABIA showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed CysDDev. Further, ABIA also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.

scholarly journals Tracking RNA with light: selection, structure, and design of fluorescence turn-on RNA aptamers

2019 ◽  
Vol 52 ◽  
Author(s):  
Robert J. Trachman ◽  
Adrian R. Ferré-D'Amaré
Keyword(s):  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Structural Diversity ◽  
Rna Aptamers ◽  
Live Cells ◽  
Rna Molecules ◽  
Turn On ◽  
Green Fluorescent ◽  
Fluorescence Turn On

AbstractFluorescence turn-on aptamers,in vitroevolved RNA molecules that bind conditional fluorophores and activate their fluorescence, have emerged as RNA counterparts of the fluorescent proteins. Turn-on aptamers have been selected to bind diverse fluorophores, and they achieve varying degrees of specificity and affinity. These RNA–fluorophore complexes, many of which exceed the brightness of green fluorescent protein and their variants, can be used as tags for visualizing RNA localization and transport in live cells. Structure determination of several fluorescent RNAs revealed that they have diverse, unrelated overall architectures. As most of these RNAs activate the fluorescence of their ligands by restraining their photoexcited states into a planar conformation, their fluorophore binding sites have in common a planar arrangement of several nucleobases, most commonly a G-quartet. Nonetheless, each turn-on aptamer has developed idiosyncratic structural solutions to achieve specificity and efficient fluorescence turn-on. The combined structural diversity of fluorophores and turn-on RNA aptamers has already produced combinations that cover the visual spectrum. Further molecular evolution and structure-guided engineering is likely to produce fluorescent tags custom-tailored to specific applications.

Silicon Quantum Dot-Based Fluorescence Turn-On Metal Ion Sensors in Live Cells

2016 ◽  
Vol 8 (36) ◽  
pp. 23953-23962 ◽  
Author(s):  
Namasivayam Dhenadhayalan ◽  
Hsin-Lung Lee ◽  
Kanchan Yadav ◽  
King-Chuen Lin ◽  
Yih-Tyng Lin ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Metal Ion ◽  
Live Cells ◽  
Ion Sensors ◽  
Turn On ◽  
Silicon Quantum Dot ◽  
Fluorescence Turn On ◽  
Metal Ion Sensors

scholarly journals A High-Performance Genetically Encoded Fluorescent Biosensor for Imaging Physiological Peroxynitrite

2020 ◽  
Author(s):  
Zhijie Chen ◽  
Shen Zhang ◽  
Xinyu Li ◽  
Hui-wang Ai
Keyword(s):  
Hydrogen Peroxide ◽  
Amino Acid ◽  
Mammalian Cells ◽  
High Performance ◽  
Live Cells ◽  
Nitrogen Species ◽  
Fluorescent Biosensor ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Noncanonical Amino Acid

SummaryPeroxynitrite is a highly reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools that permit the selective, sensitive, and non-invasive detection of peroxynitrite is essential for understanding its pathophysiological functions. Here, we present pnGFP-Ultra, a high performance, reaction-based, genetically encodable biosensor for imaging peroxynitrite in live cells. pnGFP-Ultra features a p-boronophenylalanine-modified chromophore as the sensing moiety and exhibits a remarkable 123-fold fluorescence turn-on response towards peroxynitrite while displaying virtually no cross-reaction with other reactive oxygen/nitrogen species, including hydrogen peroxide. To facilitate the expression of pnGFP-Ultra in mammalian cells, we engineered a highly efficient noncanonical amino acid (ncAA) expression system that is broadly applicable to the mammalian expression of proteins containing various ncAAs. pnGFP-Ultra robustly detected peroxynitrite production during interferon γ and lipopolysaccharide-induced immune responses in macrophages, and in amyloid β-activated primary glial cells. Thus, pnGFP-Ultra fills an important technical gap and represents an important new addition to the molecular toolbox in probing RNS biology.In BriefChen et al. report pnGFP-Ultra, a high-performance fluorescent biosensor for minimally invasive and selective imaging of peroxynitrite production in live cells.HighlightspnGFP-Ultra is a genetically encoded peroxynitrite biosensor with a 123-fold fluorescence turn-on responsepnGFP-Ultra exhibits high selectivity toward peroxynitrite, with virtually no crossreaction with hydrogen peroxideAn optimized plasmid-based system increases noncanonical amino acid incorporation in mammalian cells by >10 foldpnGFP-Ultra robustly detects peroxynitrite production in macrophages and primary glial cells

Nanostructure formation-induced fluorescence turn-on for selectively detecting protein thiols in solutions, bacteria and live cells

2015 ◽  
Vol 51 (53) ◽  
pp. 10758-10761 ◽  
Author(s):  
Guoqin Chen ◽  
Yongquan Hua ◽  
Caiwen Ou ◽  
Xiaoli Zhang ◽  
Duo Mao ◽  
...  
Keyword(s):  
Near Infrared ◽  
Live Cells ◽  
Selective Detection ◽  
Near Infrared Fluorescence ◽  
Nanostructure Formation ◽  
Turn On ◽  
Protein Thiols ◽  
Fluorescence Light ◽  
Fluorescence Turn On

A near-infrared fluorescence light-up probe has been synthesized for the selective detection of protein thiols in solutions, bacteria and live cells.

scholarly journals Fluorescence Turn-On Folding Sensor To Monitor Proteome Stress in Live Cells

2015 ◽  
Vol 137 (35) ◽  
pp. 11303-11311 ◽  
Author(s):  
Yu Liu ◽  
Xin Zhang ◽  
Wentao Chen ◽  
Yun Lei Tan ◽  
Jeffery W. Kelly
Keyword(s):  
Live Cells ◽  
Turn On ◽  
Fluorescence Turn On
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