Synthesis, In Vitro Cytotoxicity and Bleomycin‐Dependent DNA Damage Evaluation of Some Heterocyclic‐Fused Pyrimidinone Derivatives

2020 ◽  
Vol 5 (16) ◽  
pp. 4856-4861
Author(s):  
Ahmed El‐Mekabaty ◽  
Hassan A. Etman ◽  
Ahmed Mosbah ◽  
Ahmed A. Fadda
Keyword(s):  
Dna Damage ◽  
In Vitro Cytotoxicity ◽  
Damage Evaluation

In-vitro cytotoxicity, antioxidant, bleomycin-dependent DNA damage and immunomodulatory evaluation of 1-(4-acetylphenyl)-3-aryloxypyrrolidine-2, 5-dione based derivatives

2014 ◽  
Vol 23 (8) ◽  
pp. 3907-3915 ◽  
Author(s):  
Jigarkumar R. Patel ◽  
Bhaveshkumar D. Dhorajiya ◽  
Bharatkumar Z. Dholakiya ◽  
Farid A. Badria ◽  
Ahmed S. Ibrahim
Keyword(s):  
Dna Damage ◽  
In Vitro Cytotoxicity

2‐Amino‐5‐arylazothiazole‐Based Derivatives: In Vitro Cytotoxicity, Antioxidant Properties, and Bleomycin‐Dependent DNA Damage

2019 ◽  
Vol 4 (19) ◽  
pp. 5570-5576 ◽  
Author(s):  
Khalid M. Al‐Anazi ◽  
Ahmed H. Mahmoud ◽  
Mohammad AbulFarah ◽  
Ahmed A. Allam ◽  
Moustafa M. G. Fouda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Properties ◽  
In Vitro Cytotoxicity

scholarly journals NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1511 ◽  
Author(s):  
Filomena Mottola ◽  
Concetta Iovine ◽  
Marianna Santonastaso ◽  
Maria Luisa Romeo ◽  
Severina Pacifico ◽  
...  
Keyword(s):  
Dna Damage ◽  
Titanium Dioxide Nanoparticles ◽  
Medical Application ◽  
In Vitro Cytotoxicity ◽  
Target Cells ◽  
Tio2 Nps ◽  
Antimicrobial Efficiency ◽  
Potential Impact ◽  
Amniotic Cells

Titanium dioxide nanoparticles (NPs-TiO2 or TiO2-NPs) have been employed in many commercial products such as medicines, foods and cosmetics. TiO2-NPs are able to carry antibiotics to target cells enhancing the antimicrobial efficiency; so that these nanoparticles are generally used in antibiotic capsules, like lincomycin, added as a dye. Lincomycin is usually used to treat pregnancy bacterial vaginosis and its combination with TiO2-NPs arises questions on the potential effects on fetus health. This study investigated the potential impact of TiO2-NPs and lincomycin co-exposure on human amniocytes in vitro. Cytotoxicity was evaluated with trypan blue vitality test, while genotoxic damage was performed by Comet Test, Diffusion Assay and RAPD-PCR for 48 and 72 exposure hours. Lincomycin exposure produced no genotoxic effects on amniotic cells, instead, the TiO2-NPs exposure induced genotoxicity. TiO2-NPs and lincomycin co-exposure caused significant increase of DNA fragmentation, apoptosis and DNA damage in amniocytes starting from 48 exposure hours. These results contribute to monitor the use of TiO2-NPs combined with drugs in medical application. The potential impact of antibiotics with TiO2-NPs during pregnancy could be associated with adverse effects on embryo DNA. The use of nanomaterials in drugs formulation should be strictly controlled in order to minimize risks.

Monodentate Coordination of N, N′-Disubstituted Thiocarbamide Ligands: Syntheses, Structural Analyses, In Vitro Cytotoxicity and DNA Damage Studies of Cu(I) Complexes

2018 ◽  
Vol 3 (13) ◽  
pp. 3675-3679 ◽  
Author(s):  
Sunil K. Pandey ◽  
Durga P. Singh ◽  
Gaetano Marverti ◽  
R. J. Butcher ◽  
Seema Pratap
Keyword(s):  
Dna Damage ◽  
In Vitro Cytotoxicity ◽  
Monodentate Coordination

Copper (I) complexes based on novel N, N′-disubstituted thiocarbamides: Synthesis, spectroscopic, in vitro cytotoxicity, DNA damage and G0/G1 cell cycle arrest studies

2019 ◽  
Vol 491 ◽  
pp. 105-117 ◽  
Author(s):  
Sunil K. Pandey ◽  
Seema Pratap ◽  
Sandeep Pokharia ◽  
Hirdyesh Mishra ◽  
Gaetano Marverti ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Cell Cycle Arrest ◽  
In Vitro Cytotoxicity ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

Bestimmung der DNA-Schädigung in vitro

2010 ◽  
Vol 49 (S 01) ◽  
pp. S64-S68
Author(s):  
E. Dikomey
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Chromosome Aberrations ◽  
Genetic Factors ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Cell Inactivation ◽  
Repair Mechanisms ◽  
Break Repair

SummaryIonising irradiation acts primarily via induction of DNA damage, among which doublestrand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of doublestrand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about doublestrand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.

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