2‐Amino‐5‐arylazothiazole‐Based Derivatives: In Vitro Cytotoxicity, Antioxidant Properties, and Bleomycin‐Dependent DNA Damage

2019 ◽  
Vol 4 (19) ◽  
pp. 5570-5576 ◽  
Author(s):  
Khalid M. Al‐Anazi ◽  
Ahmed H. Mahmoud ◽  
Mohammad AbulFarah ◽  
Ahmed A. Allam ◽  
Moustafa M. G. Fouda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Properties ◽  
In Vitro Cytotoxicity

In-vitro cytotoxicity, antioxidant, bleomycin-dependent DNA damage and immunomodulatory evaluation of 1-(4-acetylphenyl)-3-aryloxypyrrolidine-2, 5-dione based derivatives

2014 ◽  
Vol 23 (8) ◽  
pp. 3907-3915 ◽  
Author(s):  
Jigarkumar R. Patel ◽  
Bhaveshkumar D. Dhorajiya ◽  
Bharatkumar Z. Dholakiya ◽  
Farid A. Badria ◽  
Ahmed S. Ibrahim
Keyword(s):  
Dna Damage ◽  
In Vitro Cytotoxicity

Synthesis, In Vitro Cytotoxicity and Bleomycin‐Dependent DNA Damage Evaluation of Some Heterocyclic‐Fused Pyrimidinone Derivatives

2020 ◽  
Vol 5 (16) ◽  
pp. 4856-4861
Author(s):  
Ahmed El‐Mekabaty ◽  
Hassan A. Etman ◽  
Ahmed Mosbah ◽  
Ahmed A. Fadda
Keyword(s):  
Dna Damage ◽  
In Vitro Cytotoxicity ◽  
Damage Evaluation

Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro

2017 ◽  
Vol 155 ◽  
pp. 323-331 ◽  
Author(s):  
Vladan Bajić ◽  
Biljana Spremo-Potparević ◽  
Lada Živković ◽  
Andrea Čabarkapa ◽  
Jelena Kotur-Stevuljević ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ascorbic Acid ◽  
Tio2 Nanoparticles ◽  
Antioxidant Properties ◽  
Surface Modified

scholarly journals Bioactive Icariin/β-CD-IC/Bacterial Cellulose with Enhanced Biomedical Potential

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 387
Author(s):  
Alfred Mensah ◽  
Yajun Chen ◽  
Benjamin K. Asinyo ◽  
Ebenezer Kofi Howard ◽  
Christopher Narh ◽  
...  
Keyword(s):  
Drug Release ◽  
Inclusion Complex ◽  
Bacterial Cellulose ◽  
Antioxidant Properties ◽  
Inclusion Complexation ◽  
Face Mask ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Test ◽  
Test Results

A “super” bioactive antibacterial hydrogel, Icariin-β-CD-inclusion complex/Bacterial cellulose and an equally capable counterpart Icariin-Bacterial cellulose (ICBC) were successfully produced with excellent antioxidant properties. The highly porous hydrogels demonstrated very high fluid/liquid absorption capability and were functionally active as Fourier Transform Infrared Spectrometer (FTIR) test confirmed the existence of abundant hydroxyls (-OH stretching), carboxylic acids (-CH2/C-O stretching), Alkyne/nitrile (C≡C/C≡N stretching with triple bonds) and phenol (C-H/N-O symmetric stretching) functional groups. Scanning electron microscope (SEM) and X-ray diffraction (XRD) tests confirmed a successful β-CD-inclusion complexation with Icariin with a great potential for sustained and controlled drug release. In vitro drug release test results indicated a systemic and controlled release of the drug (Icariin) from the internal cavities of the β-CD inclusion complex incorporated inside the BC matrix with high Icariin (drug) release rates. Impressive inactivation rates against Gram-negative bacteria Escherichia coli ATCC 8099 and gram-positive bacteria Staphylococcus aureus ATCC 6538; >99.19% and >98.89% respectively were recorded, as the materials proved to be non-toxic on L929 cells in the in vitro cytotoxicity test results. The materials with promising versatile multipurpose administration of Icariin for wound dressing (as wound dressers), can also be executed as implants for tissue regeneration, as well as face-mask for cosmetic purposes.

scholarly journals Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment

2020 ◽  
Vol 9 (6) ◽  
pp. 1638 ◽  
Author(s):  
Laura Governini ◽  
Rosetta Ponchia ◽  
Paolo Giovanni Artini ◽  
Elena Casarosa ◽  
Ilaria Marzi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Phosphorylation ◽  
Oxidative Damage ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Antioxidant Properties ◽  
Atp Production ◽  
In Vitro Treatment

Semen samples are known to contain abnormal amounts of reactive oxygen species (ROS) and oxygen free radicals; therefore, the identification of antioxidant molecules able to counteract the oxidative damage caused by ROS is foresight. Indeed, improving semen quality in terms of motility and reduction in DNA damage, can significantly improve the fertilization potential of sperm in vitro. To this regard, myo-inositol, based on its antioxidant properties, has been reported to be effective in improving sperm quality and motility in oligoasthenozoospermic patients undergoing assisted reproduction techniques when used as a dietary supplementation. Moreover, in vitro treatment demonstrated a direct relationship between myo-inositol, mitochondrial membrane potential and sperm motility. This experimental study aimed to evaluate the effects of myo-inositol (Andrositol-lab) in vitro treatment on sperm motility, capacitation, mitochondrial oxidative phosphorylation and DNA damage. Our results demonstrate that myo-inositol induces a significant increase in sperm motility and in oxygen consumption, the main index of oxidative phosphorylation efficiency and ATP production, both in basal and in in vitro capacitated samples. Moreover, we provide evidence for a significant protective role of myo-inositol against oxidative damage to DNA, thus supporting the in vitro use of myo-inositol in assisted reproductive techniques. Even if further studies are needed to clarify the mechanisms underlying the antioxidant properties of myo-inositol, the present findings significantly extend our knowledge on human male fertility and pave the way to the definition of evidence-based guidelines, aiming to improve the in vitro procedure currently used in ART laboratory for sperm selection.

scholarly journals Investigation of in vitro cytotoxicity of the redox state of ionic iron in neuroblastoma cells

2012 ◽  
Vol 03 (03) ◽  
pp. 301-310 ◽  
Author(s):  
Ajay Vikram Singh ◽  
Varun Vyas ◽  
Erica Montani ◽  
Daniele Cartelli ◽  
Dario Parazzoli ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Iron Chelation ◽  
Microscopic Analysis ◽  
Chelating Agent ◽  
In Vitro Cytotoxicity ◽  
Ionic State ◽  
Morphological Studies ◽  
Ionic Iron

ABSTRACT Background: there is an intimate relation between transition metals and cell homeostasis due to the physiological necessity of metals in vivo. Particularly, iron (ferrous and ferric state) is utilized in many physiological processes of the cell but in excess has been linked with negative role contributing in many neurodegenerative processes. Objective: the aim of this study was to investigate which oxidation state of ionic iron (Ferrous (II) versus Ferric (III)) is more toxic to neuronal cells (SHSY5Y). Materials and Methods: The neuroblastoma (SHSY5Y) cells were exposed to varying concentration of ferric and ferrous iron. Morphological studies using immunofluorescence staining and microscopic analysis as confirmed by intracellular glutathione (GSH) test demonstrated oxidative stress to cells in iron microenvironment. In addition, MTT assay was performed to evaluate the viability and metabolic state of the cells. Results: the results showed that ferrous form has significantly higher toxicity compared to the ferric ionic state of higher concentration. In addition, microscopic analysis shows cell fenestration at higher concentrations and swelling at intermediate ferric dosages as demonstrated by atomic force microscopy (AFM). Interestingly, the addition of a differentiation inducing factor, trans-retinoic rcid (RA) retains significant viability and morphological features of the cells irrespective of the ionic state of the iron. AFM images revealed clustered aggregates arising from iron chelation with RA. Conclusions: the results indicate that Fe (II) has more toxic effects on cells. In addition, it could be an interesting finding with respect to the antioxidant properties of RA as a chelating agent for the neurodegenerative therapeutics.

scholarly journals The Effect ofLeonurus sibiricusPlant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Przemysław Sitarek ◽  
Ewa Skała ◽  
Halina Wysokińska ◽  
Marzena Wielanek ◽  
Janusz Szemraj ◽  
...  
Keyword(s):  
Dna Damage ◽  
Phenolic Compounds ◽  
Plant Extracts ◽  
Cho Cells ◽  
Oxidative Dna Damage ◽  
Antioxidant Properties ◽  
Chinese Hamster ◽  
Antioxidant Genes ◽  
Aerial Parts

Leonurus sibiricusL. has been used as a traditional and medicinal herb for many years in Asia and Europe. This species is known to have antibacterial, anti-inflammatory, and antioxidant activity and has demonstrated a reduction of intracellular reactive oxygen species. All tested extracts ofL. sibiricusshowed protective and DNA repair stimulating effects in Chinese hamster ovary (CHO) cells exposed to H2O2. Preincubation of the CHO cells with 0.5 mg/mL of plant extracts showed increased expression level of antioxidant genes (SOD2, CAT,andGPx). LC-MS/MS and HPLC analyses revealed the presence of nine phenolic compounds inL. sibiricusplant extracts: catechin, verbascoside, two flavonoids (quercetin and rutin), and five phenolic acids (4-hydroxybenzoic acid, chlorogenic acid, caffeic acid,p-coumaric acid, and ferulic acid). The roots and aerial parts ofin vitro L. sibiricusplant extracts, which had the strongest antioxidant properties, may be responsible for stimulating CHO cells to repair oxidatively induced DNA damage, as well as protecting DNA via enhanced activation of the antioxidant genes (SOD2, CAT,andGPx) regulating intracellular antioxidant capacity. The content of phenolic compounds inin vitroraised plants was greater than the levels found in plants propagated from seeds.

scholarly journals NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1511 ◽  
Author(s):  
Filomena Mottola ◽  
Concetta Iovine ◽  
Marianna Santonastaso ◽  
Maria Luisa Romeo ◽  
Severina Pacifico ◽  
...  
Keyword(s):  
Dna Damage ◽  
Titanium Dioxide Nanoparticles ◽  
Medical Application ◽  
In Vitro Cytotoxicity ◽  
Target Cells ◽  
Tio2 Nps ◽  
Antimicrobial Efficiency ◽  
Potential Impact ◽  
Amniotic Cells

Titanium dioxide nanoparticles (NPs-TiO2 or TiO2-NPs) have been employed in many commercial products such as medicines, foods and cosmetics. TiO2-NPs are able to carry antibiotics to target cells enhancing the antimicrobial efficiency; so that these nanoparticles are generally used in antibiotic capsules, like lincomycin, added as a dye. Lincomycin is usually used to treat pregnancy bacterial vaginosis and its combination with TiO2-NPs arises questions on the potential effects on fetus health. This study investigated the potential impact of TiO2-NPs and lincomycin co-exposure on human amniocytes in vitro. Cytotoxicity was evaluated with trypan blue vitality test, while genotoxic damage was performed by Comet Test, Diffusion Assay and RAPD-PCR for 48 and 72 exposure hours. Lincomycin exposure produced no genotoxic effects on amniotic cells, instead, the TiO2-NPs exposure induced genotoxicity. TiO2-NPs and lincomycin co-exposure caused significant increase of DNA fragmentation, apoptosis and DNA damage in amniocytes starting from 48 exposure hours. These results contribute to monitor the use of TiO2-NPs combined with drugs in medical application. The potential impact of antibiotics with TiO2-NPs during pregnancy could be associated with adverse effects on embryo DNA. The use of nanomaterials in drugs formulation should be strictly controlled in order to minimize risks.

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