From Concentrated Dispersion to Solid β‐Cyclodextrin Polymer‐Capped Silver Nanoparticle Formulation: A Trojan Horse Against  Escherichia coli

2019 ◽  
Vol 4 (34) ◽  
pp. 10092-10096 ◽  
Author(s):  
Rudy Martin‐Trasanco ◽  
Giovanna Anziani‐Ostuni ◽  
Hilda Esperanza Esparza‐Ponce ◽  
Pedro Ortiz ◽  
María E. Montero‐Cabrera ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticle ◽  
Trojan Horse ◽  
Cyclodextrin Polymer ◽  
Nanoparticle Formulation ◽  
Concentrated Dispersion

scholarly journals Facile Route of Fabricating Long-Term Microbicidal Silver Nanoparticle Clusters against Shiga Toxin-Producing Escherichia coli O157:H7 and Candida auris

Coatings ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 28
Author(s):  
Sheeana Gangadoo ◽  
Aaron Elbourne ◽  
Alexander E. Medvedev ◽  
Daniel Cozzolino ◽  
Yen B. Truong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticle ◽  
Antimicrobial Properties ◽  
Reduction Reaction ◽  
Fungal Species ◽  
Escherichia Coli O157 ◽  
Candida Auris ◽  
Surface Design ◽  
Marine Applications

Microbial contamination remains a significant issue for many industrial, commercial, and medical applications. For instance, microbial surface contamination is detrimental to numerous aspects of food production, infection transfer, and even marine applications. As such, intense scientific interest has focused on improving the antimicrobial properties of surface coatings via both chemical and physical routes. However, there is a lack of synthetic coatings that possess long-term microbiocidal performance. In this study, silver nanoparticle cluster coatings were developed on copper surfaces via an ion-exchange and reduction reaction, followed by a silanization step. The durability of the microbiocidal activity for these develped surfaces was tested against pathogenic bacterial and fungal species, specifically Escherichia coli O157:H7 and Candida auris, over periods of 1- and 7-days. It was observed that more than 90% of E. coli and C. auris were found to be non-viable following the extended exposure times. This facile material fabrication presents as a new surface design for the production of durable microbicidal coatings which can be applied to numerous applications.

scholarly journals Microbiological Research of the Effects of EVODROP Silver Nanoparticle on Escherichia coli, Enterococci and Coliforms

2020 ◽  
pp. 22-31
Author(s):  
Nedyalka Valcheva ◽  
Ignat Ignatov ◽  
Fabio Huether
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Influenza A ◽  
Viral Infections ◽  
Membrane Damage ◽  
Genetic Material ◽  
Nano Particles ◽  
Before And After ◽  
Physical Changes

The aim of the study was to analyze the microbiological effects of EVODROP Silver Nanoparticle. A comparative analysеs of the number of bacteria of a given species before and after influence wеre performed. Nanoparticle Silver makes physical changes in the bacterial membrane, like the membrane damage, which can lead to cellular contents leakage and bacterial death [1,2,3]. There are effects of Nanoparticle Silver against SARS-CoV-2. The coronavirus gets replicated via copying of the genetic material using the enzyme RNA-dependent RNA polymerase [4,5]. The membranes of the cells of mammals don’t have peptidoglycans and Ag+ has not inhibiting effect [6]. In such a way it prevents the further alteration of the enzymes. In Japanese study there are proofs that Nanoparticle Silver inhibiting extracellular SARS-CoV-2 at concentrations ranging between 1 and 10 ppm while cytotoxic effect was observed at concentrations of 20 ppm and above [7]. There is objective research of the effects of Nanoparticle Silver on the virus SARS-CoV-2 with Inhalation in Israel [8]. The author of the device EVOhygiene Fabio Huether performs liquid with concentration 20 ppm nano silver and size of nano particles 5 nm. The virucidal activity of silver nanoparticles (AgNPs) allows its wider application in the annihilation or amelioration of several viral infections such as Poliovirus type-1, Coxsackievirus B3, influenza A virus, SARS-CoV-2 etc. [9] There is publication in Nature Nanotechnology for the effects of Silver Nanoparticles as disinfectant against SARS-CoV-2 [10]. The research show effects of EVODROP Silver Nanoparticle on the following bacteria Escherichia coli, Enterococci and Coliforms.

scholarly journals Enhanced Silver Nanoparticle Synthesis by Escherichia Coli Transformed with Candida Albicans Metallothionein Gene

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4180 ◽  
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Silver Nanoparticle ◽  
Large Scale ◽  
Nanoparticle Synthesis ◽  
Scale Production ◽  
E Coli ◽  
Metallothionein Gene ◽  
Silver Nanoparticle Synthesis

In this study, the metallothionein gene of Candida albicans (C. albicans) was assembled by polymerase chain reaction (PCR), inserted into pUC19 vector, and further transformed into Escherichia coli (E. coli) DH5α cells. The capacity of these recombinant E. coli DH5α cells to synthesize silver nanoparticles was examined. Our results demonstrated that the expression of C. albicans metallothionein in E. coli promoted the bacterial tolerance to metal ions and increased yield of silver nanoparticle synthesis. The compositional and morphological analysis of the silver nanoparticles revealed that silver nanoparticles synthesized by the engineered E. coli cells are around 20 nm in size, and spherical in shape. Importantly, the silver nanoparticles produced by the engineered cells were more homogeneous in shape and size than those produced by bacteria lack of the C. albicans metallothionein. Our study provided preliminary information for further development of the engineered E. coli as a platform for large-scale production of uniform nanoparticles for various applications in nanotechnology.

scholarly journals The anti-biofilm effect of silver-nanoparticle-decorated quercetin nanoparticles on a multi-drug resistant Escherichia coli strain isolated from a dairy cow with mastitis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5711 ◽  
Author(s):  
Lumin Yu ◽  
Fei Shang ◽  
Xiaolin Chen ◽  
Jingtian Ni ◽  
Li Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Silver Nanoparticle ◽  
Dairy Cow ◽  
Biofilm Formation ◽  
Bovine Mastitis ◽  
Economic Losses ◽  
Drug Resistant ◽  
Ag Nps ◽  
E Coli

Background Escherichia coli is an important opportunistic pathogen that could cause inflammation of the udder in dairy cows resulting in reduced milk production and changes in milk composition and quality, and even death of dairy cows. Therefore, mastitis is the main health issue which leads to major economic losses on dairy farms. Antibiotics are routinely used for the treatment of bovine mastitis. The ability to form biofilm increases the antibiotic resistance of E. coli. Nanoparticles (NPs), a nanosized, safe, and highly cost-effective antibacterial agent, are potential biomedical tools. Given their antibacterial activities, silver nanoparticles (Ag NPs) have a broad range of applications. Methods In this study, we performed antibacterial activity assays, biofilm formation assays, scanning electron microscopy (SEM) experiments, and real-time reverse transcription PCR (RT-PCR) experiments to investigate the antibacterial and anti-biofilm effect of quercetin, Ag NPs, and Silver-nanoparticle-decorated quercetin nanoparticles (QA NPs) in E. coli strain ECDCM1. Results In this study, QA NPs, a composite material combining Ag NPs and the plant-derived drug component quercetin, exhibited stronger antibacterial and anti-biofilm properties in a multi-drug resistant E. coli strain isolated from a dairy cow with mastitis, compared to Ag NPs and Qe. Discussion This study provides evidence that QA NPs possess high antibacterial and anti-biofilm activities. They proved to be more effective than Ag NPs and Qe against the biofilm formation of a multi-drug resistant E. coli isolated from cows with mastitis. This suggests that QA NPs might be used as a potential antimicrobial agent in the treatment of bovine mastitis caused by E. coli.

scholarly journals Effect of Chitosan and Alginate Concentration on Size and Bactericidal Activity against Escherichia coli of Chitosan/Alginate/Silver Nanoparticle Beads

2016 ◽  
Vol 855 ◽  
pp. 54-59
Author(s):  
Pawika Mahasawat ◽  
Ketsarin Hlongkeaw ◽  
Sutthida Charoenrit
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Bactericidal Activity ◽  
Minimum Bactericidal Concentration ◽  
Alginate Beads ◽  
Alginate Concentration ◽  
Antibacterial Material

Silver nanoparticles have been used in combination with biological polymer for antibacterial application. This study prepared chitosan/alginate/AgNP beads with varying chitosan and alginate concentration to use as an antibacterial material. The sizes of neat beads were larger (1286 ± 172, 1344 ± 142 and 1529 ± 73 μm for C1, C2 and C3, respectively) with increasing concentration of chitosan and alginate. Moreover, smaller beads were observed for the chitosan/alginate/AgNP beads, in which their sizes were 1151 ± 201, 1261 ± 204 and 1324 ± 198 µm for S1, S2 and S3, respectively, when compared to the chitosan/alginate beads. Furthermore, the minimum bactericidal concentration (MBC) of chitosan/alginate/AgNP beads against E. coli was 10, 10 and 3 µg/ml for S1, S2 and S3, respectively. This study suggested that the beads with the higher concentration of chitosan and alginate resulted in the greater bactericidal activity. Therefore, the chitosan/alginate/AgNP beads prepared in this study showed the bactericidal activity which can be used for antibacterial application.

scholarly journals Comparative Study of the Silver Nanoparticle Synthesis Ability and Antibacterial Activity of the Piper Betle L. and Piper Sarmentosum Roxb. Extracts

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Ngoc Hoi Nguyen ◽  
Tran Thi Yen Nhi ◽  
Ngo Thi Van Nhi ◽  
Tran Thi Thu Cuc ◽  
Pham Minh Tuan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Silver Nanoparticle ◽  
Nanoparticle Synthesis ◽  
Reducing Power ◽  
Piper Betle ◽  
Antibacterial And Antifungal Activities ◽  
Dpph Scavenging ◽  
Antibacterial And Antifungal ◽  
Silver Nanoparticle Synthesis

Piper betle (P. betle) and Piper sarmentosum (P. sarmentosum) are the two members of the Piper genus, have been reported to be rich in phytochemicals and essential oils, which showed strong reducing power, antibacterial, and antifungal activities. P. betle recently has been studied and applied in several commercial products in the antimicrobial respect, meanwhile its relatives, P. sarmentosum has been lesser-known in this field. In this study, the two Piper species—P. betle and P. sarmentosum were studied to compare their ability in silver nanoparticle synthesis and efficacy in antibacterial activity. P. betle and P. sarmentosum were extracted by distilled water at different temperatures and times. Subsequently, their total reducing capacity was determined by DPPH scavenging and Folin-Ciocalteu assays to choose the appropriate extraction conditions. The silver nanoparticle solutions prepared by the extracts of P. betle (Pb.ext) and P. sarmentosum (Ps.ext) were characterized by Dynamic light scattering (DLS), Zeta potential, UV-vis, and Fourier-transform infrared (FTIR) measurements. Finally, the antibacterial activity of the synthesized silver nanoparticle solutions was tested against Escherichia coli using the agar diffusion well–variant method. The Pb.ext showed stronger reducing power with higher total polyphenol content (~125 mg GAE/mL extract) and better DPPH activity (IC50~1.45%). Both the green synthesized silver nanoparticle solutions (Pb.AgNP and Ps.AgNP) performed significantly stronger antibacterial activity on Escherichia coli compared to their initial extracts. Antibacterial tests revealed that Ps.AgNP showed remarkably better growth inhibition activity as compared to Pb.AgNP. This study would contribute useful and important information to the development of antibacterial products based on green synthesized silver nanoparticles fabricated by the extracts of P. betle and P. sarmentosum.

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