A facile, one-step method for the determination of accessible surface primary amino groups on solid carriers

2012 ◽  
Vol 44 (10) ◽  
pp. 1309-1313 ◽  
Author(s):  
Zhenyuan Qu ◽  
Hong Xu ◽  
Puwen Ning ◽  
Hongchen Gu
Keyword(s):  
Amino Groups ◽  
Step Method ◽  
One Step ◽  
Primary Amino ◽  
Accessible Surface

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

One-step method for plasma determination of ibuprofen by chemiluminescence-coupled ultrafiltration and application in a pharmacokinetic study

Luminescence ◽  
2011 ◽  
Vol 27 (5) ◽  
pp. 371-378 ◽  
Author(s):  
Xunyu Xiong ◽  
Qunzheng Zhang ◽  
Yefei Nan ◽  
Xuefan Gu
Keyword(s):  
Pharmacokinetic Study ◽  
Step Method ◽  
One Step

A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase

1971 ◽  
Vol 41 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Jonathan J. Goldthwaite ◽  
Lawrence Bogorad
Keyword(s):  
Step Method ◽  
One Step

scholarly journals The Blocked Amino-Terminal Peptide of Feather Keratin

1971 ◽  
Vol 24 (1) ◽  
pp. 179 ◽  
Author(s):  
IJ O'donnell
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Amino Groups ◽  
Amino Terminal ◽  
Acetyl Content ◽  
Electrophoretic Patterns ◽  
Isolation And Characterization ◽  
Primary Amino

Proteins extracted from reduced and carboxymethylated feather keratins (SCM-keratins) have been studied by Harrap and Woods (1964a, 1964b, 1967). They have demonstrated the presence of electrophoretic heterogeneity amongst the proteins and have obtained a molecular weight of approximately 11,000 in agreement with earlier work of Woodin (1954). There was no indication of marked heterogeneity with respect to size. Using acid hydrolysis and determination of acetic acid produced they found an acetyl content of 1 �30 molesj104 g in the rachis off owl feathers. These were thought to be attached to primary amino groups since there were no O-acetyl groups. In the present paper the isolation and characterization of the predominant, and probably sole, amino-terminal tripeptide from goose feather calamus is described. Goose feather calamus was chosen because its extracted proteins had one of the simplest electrophoretic patterns of proteins from the feathers of a number of species (Harrap and Woods 1967).

Colorimetric determination of reactive primary amino groups of macro- and microsolid supports

1993 ◽  
Vol 38 (1-2) ◽  
pp. 15-25 ◽  
Author(s):  
Philemon E. Tyllianakis ◽  
Sotiris E. Kakabakos ◽  
Gregory P. Evangelatos ◽  
Dionyssis S. Ithakissios
Keyword(s):  
Colorimetric Determination ◽  
Amino Groups ◽  
Primary Amino

New Method for the Determination of Free Amino Groups in Intact Pure Proteins: Relationship to Available Lysine

1976 ◽  
Vol 59 (6) ◽  
pp. 1251-1254
Author(s):  
James M Purcell ◽  
Daniel J Quimby ◽  
James R Cavanaugh
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Free Amino ◽  
Amino Group ◽  
Amino Groups ◽  
Primary Amino ◽  
Available Lysine ◽  
Β Lactoglobulin ◽  
Group Concentration

Abstract A new rapid method for the quantitative and routine determination of free amino groups in intact pure proteins has been developed. Primary amino groups are labeled with fluorescamine and the labeled groups are detected by absorption spectroscopy in the range 375–390 nm. The amino group concentration can be determined in a few minutes without hydrolyzing the labeled protein and extracting a lysine derivative. The method was tested with the following proteins: lysozyme, α-lactalbumin, β-lactoglobulin, bovine serum albumin, ribonuclease, ribonuclease-S-peptide, and αsl-rasciii B. Application of this method to the estimation of available lysine is discussed.

A sensitive one-step method for quantitative detection of α-amylase in serum and urine using a personal glucose meter

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 1161-1165 ◽  
Author(s):  
Qing Wang ◽  
Hui Wang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Rongjuan Liu ◽  
...  
Keyword(s):  
Amylase Activity ◽  
Direct Determination ◽  
Quantitative Detection ◽  
Step Method ◽  
Personal Glucose Meter ◽  
One Step ◽  
Glucose Meter ◽  
Serum And Urine

A one-step assay for direct determination of α-amylase activity in serum and urine was developed using a portable personal glucose meter.

Sign in / Sign up

Export Citation Format

Share Document