Imaging the surface details of red blood cells with atomic force microscopy

2002 ◽  
Vol 33 (2) ◽  
pp. 118-121 ◽  
Author(s):  
Robert Nowakowski ◽  
Paul Luckham
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

Investigation of the influence of phenosan, ichphan-10, and melafen on red blood cells in vivo by atomic force microscopy

2011 ◽  
Vol 441 (1) ◽  
pp. 245-247 ◽  
Author(s):  
V. I. Binyukov ◽  
O. M. Alekseeva ◽  
E. M. Mil ◽  
A. A. Albantova ◽  
S. G. Fattachov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Usage of atomic force microscopy for detection of the damaging effect of CdCl2 on red blood cells membrane

2021 ◽  
Vol 208 ◽  
pp. 111683
Author(s):  
E.L. Demchenkov ◽  
A.A. Nagdalian ◽  
R.O. Budkevich ◽  
N.P. Oboturova ◽  
A.I. Okolelova
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

Ultrastructural changes in red blood cells in aortic dissection patients under extracorporeal circulation: Atomic force microscopy study

2018 ◽  
Vol 41 (8) ◽  
pp. 452-459
Author(s):  
Li Deng ◽  
Hui Yang ◽  
Weixin Meng ◽  
Yue Chen ◽  
Guang Hu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Aortic Dissection ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Extracorporeal Circulation ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scanning Electron

Purpose: During extracorporeal circulation in heart surgery, blood is exposed to non-physiological conditions, such as high shear stress, foreign surfaces, turbulence, and hypothermia. These factors cause damage to the red blood cells, which is manifested by immediate and delayed hemolysis or some changes in the mechanical properties of red blood cells, defined as sublethal trauma. Unfortunately, sublethal trauma is hard to detect, and there is not enough morphological evidence regarding red blood cell sublethal trauma. In this study, red blood cell sublethal trauma was observed after extracorporeal circulation by describing ultrastructural changes in red blood cell membranes using atomic force microscopy and scanning electron microscopy. Methods: Venous blood (2 mL) was collected into heparin tubes from preoperative, intraoperative and postoperative aortic dissection patients for comparison with blood from healthy patients. The red blood cell morphological study (malformations percentage, diameter, height, concavity, and roughness) was performed with scanning electron microscopy and atomic force microscopy. Results: Scanning electron microscopy and atomic force microscopy imaging analysis revealed that the red blood cell shape changed during extracorporeal circulation and that the red blood cell malformation percentage in the postoperative group was higher than those in the preoperative and intraoperative groups. Most morphological parameters had no obvious changes, except roughness (Ra and Rq) in aortic dissection patients. Atomic force microscopy quantitative analysis indicated that the roughness of red blood cell membranes increased during extracorporeal circulation. Conclusions: This study demonstrates that ultrastructural morphological damage occurs to red blood cells membranes due to extracorporeal circulation in aortic dissection patients. In addition, we provided a new parameter (Ra and Rq) to evaluate red blood cell sublethal trauma.

Multispectral Atomic Force Microscopy-Infrared Nano-Imaging of Malaria Infected Red Blood Cells

2018 ◽  
Vol 90 (5) ◽  
pp. 3140-3148 ◽  
Author(s):  
David Perez-Guaita ◽  
Kamila Kochan ◽  
Mitchell Batty ◽  
Christian Doerig ◽  
Jose Garcia-Bustos ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Nano Imaging
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