scholarly journals Three-dimensional laser scanning two-photon fluorescence confocal microscopy of polymer materials using a new, efficient upconverting fluorophore

Scanning ◽  
2006 ◽  
Vol 18 (8) ◽  
pp. 562-566 ◽  
Author(s):  
J. D. Bhawalkar ◽  
J. Swiatkiewicz ◽  
S. J. Pan ◽  
J. K. Samarabandu ◽  
W. S. Liou ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Polymer Materials ◽  
Two Photon ◽  
Fluorescence Confocal Microscopy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence Microendoscopy

2004 ◽  
Vol 92 (5) ◽  
pp. 3121-3133 ◽  
Author(s):  
Juergen C. Jung ◽  
Amit D. Mehta ◽  
Emre Aksay ◽  
Raymond Stepnoski ◽  
Mark J. Schnitzer
Keyword(s):  
Laser Scanning ◽  
Frame Rate ◽  
Mammalian Brain ◽  
Cell Dynamics ◽  
Ca1 Neurons ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Rats And Mice ◽  
Photon Fluorescence

One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells in vivo. Both one- and two-photon fluorescence microendoscopy are based on compound gradient refractive index (GRIN) lenses that are 350–1,000 μm in diameter and provide micron-scale resolution. One-photon microendoscopy allows full-frame images to be viewed by eye or with a camera, and is well suited to fast frame-rate imaging. Two-photon microendoscopy is a laser-scanning modality that provides optical sectioning deep within tissue. Using in vivo microendoscopy we acquired video-rate movies of thalamic and CA1 hippocampal red blood cell dynamics and still-frame images of CA1 neurons and dendrites in anesthetized rats and mice. Microendoscopy will help meet the growing demand for in vivo cellular imaging created by the rapid emergence of new synthetic and genetically encoded fluorophores that can be used to label specific brain areas or cell classes.

scholarly journals Cardiac muscle organization revealed in 3-D by imaging whole-mount mouse hearts using two-photon fluorescence and confocal microscopy

BioTechniques ◽  
2015 ◽  
Vol 59 (5) ◽  
Author(s):  
Mayandi Sivaguru ◽  
Glenn Fried ◽  
Barghav S. Sivaguru ◽  
Vignesh A. Sivaguru ◽  
Xiaochen Lu ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Cardiac Muscle ◽  
Two Photon ◽  
Whole Mount ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
Sign in / Sign up

Export Citation Format

Share Document