HIV‐1 viral load testing in resource‐limited settings: Challenges and solutions for specimen integrity

2020 ◽  
Author(s):  
Howard Newman ◽  
Diana Hardie
Keyword(s):  
Viral Load ◽  
Load Testing ◽  
Resource Limited Settings ◽  
Viral Load Testing ◽  
Resource Limited ◽  
Hiv 1

Evaluation of two commercially available alternatives for HIV-1 viral load testing in resource-limited settings

2007 ◽  
Vol 146 (1-2) ◽  
pp. 178-187 ◽  
Author(s):  
Kim Steegen ◽  
Stanley Luchters ◽  
Nancy De Cabooter ◽  
Jacqueline Reynaerts ◽  
Kishor Mandaliya ◽  
...  
Keyword(s):  
Viral Load ◽  
Load Testing ◽  
Resource Limited Settings ◽  
Viral Load Testing ◽  
Resource Limited ◽  
Hiv 1

scholarly journals The case for viral load testing in adolescents in resource-limited settings

2017 ◽  
Vol 20 ◽  
pp. e25002 ◽  
Author(s):  
Rebecca Marcus ◽  
Rashida A Ferrand ◽  
Katharina Kranzer ◽  
Linda-Gail Bekker
Keyword(s):  
Viral Load ◽  
Load Testing ◽  
Resource Limited Settings ◽  
Viral Load Testing ◽  
Resource Limited

scholarly journals Monitoring the Quality of HIV-1 Viral Load Testing through a Proficiency Testing Program Using Dried Tube Specimens in Resource-Limited Settings

2015 ◽  
Vol 53 (4) ◽  
pp. 1129-1136 ◽  
Author(s):  
Shon Nguyen ◽  
Artur Ramos ◽  
Joy Chang ◽  
Bin Li ◽  
Vedapuri Shanmugam ◽  
...  
Keyword(s):  
Viral Load ◽  
Proficiency Testing ◽  
Good Alternative ◽  
Cold Chain ◽  
Quality Assurance Program ◽  
Load Testing ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Proficiency Scores ◽  
Hiv 1

HIV-1 viral load (VL) levels are used for monitoring disease progression and antiretroviral therapy outcomes in HIV-infected patients. To assess the performance of laboratories conducting HIV-1 VL testing in resource-limited settings, the U.S. Centers for Disease Control and Prevention implemented a voluntary, free-of-charge, external quality assurance program using dried tube specimens (DTSs). Between 2010 and 2012, DTS proficiency testing (PT) panels consisting of 5 specimens were distributed at ambient temperature to participants. The results from the participants (n≥ 6) using the same assay were grouped, analyzed, and graded as acceptable within a group mean ± 3 standard deviations. Mean proficiency scores were calculated by dividing the combined PT scores by the number of testing cycles using a linear regression model. Between 2010 and 2012, the number of participants enrolled increased from 32 in 16 countries to 114 in 44 countries. A total of 78.2% of the participants reported results using 10 different VL assays. The rates of reporting of acceptable results by the participants were 96.6% for the Abbott assay, 96.3% for the Roche Cobas assay, 94.5% for the Roche Amplicor assay, 93.0% for the Biocentric assay, and 89.3% for the NucliSens assay. The overall mean proficiency scores improved over time (P= 0.024). DTSs are a good alternative specimen type to plasma specimens for VL PT programs, as they do not require cold chain transportation and can be used on PCR-based assays. Our data suggest that the CDC HIV-1 VL PT program using DTSs positively impacts the testing performance of the participants, which might translate into better and more accurate VL testing services for patients.

scholarly journals Viral load testing in a resource-limited setting: quality control is critical

2011 ◽  
Vol 14 (1) ◽  
pp. 23-23 ◽  
Author(s):  
Jane Greig ◽  
Philipp du Cros ◽  
Derryck Klarkowski ◽  
Clair Mills ◽  
Steffen Jørgensen ◽  
...  
Keyword(s):  
Quality Control ◽  
Viral Load ◽  
Resource Limited Setting ◽  
Load Testing ◽  
Viral Load Testing ◽  
Resource Limited ◽  
Limited Setting
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