Matrix Phase Fractionation: Investigating the Compromise Between Dynamic Range Of Analyte Extraction and Spatial Resolution In Imaging Mass Spectrometry

2021 ◽  
Author(s):  
Matthew B. O’Rourke ◽  
Barney Viengkhou ◽  
Caine C. Smith ◽  
Lorenz Sonderegger ◽  
Matthew P. Padula ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Dynamic Range ◽  
Imaging Mass Spectrometry ◽  
Matrix Phase

scholarly journals Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry

2020 ◽  
Author(s):  
Boone Prentice ◽  
Daniel Ryan ◽  
Kerri Grove ◽  
D. Shannon Cornett ◽  
Richard Caprioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Imaging Mass Spectrometry ◽  
Quadrupole Mass Filter ◽  
Ion Accumulation ◽  
Quadrupole Mass ◽  
Mass Filter ◽  
Tissue Samples ◽  
Ion Species

In the analysis of biological tissue by imaging mass spectrometry (IMS), the limit of detection and dynamic range are of paramount importance in obtaining experimental results that provide insight into underlying biological processes. Many important biomolecules are present in the tissue milieu in low concentrations and in complex mixtures with other compounds of widely ranging abundances, challenging the limits of analytical technologies. In many IMS experiments, the ion signal can be dominated by a few highly abundant ion species. On trap-based instrument platforms that accumulate ions prior to mass analysis, these high abundance ions can diminish the detection and dynamic range of lower abundance ions. Herein, we characterize two strategies for combating these challenges during IMS experiments on a hybrid QqFT-ICR MS. In one iteration, the mass resolving capabilities of a quadrupole mass filter are used to selectively enrich for ions of interest via a technique previously termed continuous accumulation of selected ions (CASI). Secondly, we have introduced a supplemental dipolar AC waveform to the quadrupole mass filter of a commercial QqFT-ICR mass spectrometer to perform selected ion ejection prior to the ion accumulation region. This setup allows the selective ejection of the most abundant ion species prior to ion accumulation, thereby greatly improving the molecular depth with which IMS can probe tissue samples.<br>

scholarly journals Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry

2020 ◽  
Vol 92 (19) ◽  
pp. 13092-13100 ◽  
Author(s):  
Boone M. Prentice ◽  
Daniel J. Ryan ◽  
Kerri J. Grove ◽  
D. Shannon Cornett ◽  
Richard M. Caprioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Range Expansion ◽  
Dynamic Range ◽  
Imaging Mass Spectrometry ◽  
Gas Phase Ion

scholarly journals Standard Reticle Slide To Objectively Evaluate Spatial Resolution and Instrument Performance in Imaging Mass Spectrometry

2016 ◽  
Vol 88 (14) ◽  
pp. 7302-7311 ◽  
Author(s):  
Faizan Zubair ◽  
Boone M. Prentice ◽  
Jeremy L. Norris ◽  
Paul E. Laibinis ◽  
Richard M. Caprioli
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
Imaging Mass Spectrometry ◽  
Instrument Performance
Sign in / Sign up

Export Citation Format

Share Document