Novel vacuum stable ketone-based matrices for high spatial resolution MALDI imaging mass spectrometry

2018 ◽  
Vol 53 (10) ◽  
pp. 1005-1012 ◽  
Author(s):  
Junhai Yang ◽  
Jeremy L. Norris ◽  
Richard Caprioli
Keyword(s):  
Mass Spectrometry ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

scholarly journals Optimized Workflow for High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone

2021 ◽  
Author(s):  
Christopher J. Good ◽  
Elizabeth K. Neumann ◽  
Casey E. Butrico ◽  
James E. Cassat ◽  
Richard M. Caprioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Ims ◽  
Fresh Frozen ◽  
Frozen Bone

Bone and bone marrow are vital to mammalian structure, movement, and immunity. These tissues are also commonly subjected to pathological alterations giving rise to debilitating diseases like rheumatoid arthritis, osteoporosis, osteomyelitis, and cancer. Technologies such as matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) enable the discovery of spatially resolved chemical information in biological tissue samples to help elucidate the complex molecular processes underlying pathology. Traditionally, preparation of native osseous tissue for MALDI IMS has been difficult due to the mineralized composition and heterogenous morphology of the tissue, and compensation for these challenges with decalcification and fixation protocols can remove or delocalize molecular species. Here, sample preparation methods were advanced to enable multimodal MALDI IMS of undecalcified, fresh-frozen murine femurs allowing the distribution of endogenous lipids to be linked to specific tissue structures and cell types. Adhesive-bound bone sections were mounted onto ITO coated glass slides with a microscopy-compatible glue and freeze-dried to minimize artificial bone marrow damage. Subliming matrix does not induce further bone marrow cracks, and recrystallizing the deposited matrix improves lipid signal. High spatial resolution (10 μm) MALDI IMS was leveraged to characterize lipid distributions in fresh-frozen bone, and complementary microscopy modalities aided tissue and cell assignments. For example, various phosphatidylcholines localize to bone marrow, adipose tissue, marrow adipose tissue, and muscle. Furthermore, we discovered that [sphingomyelin(42:1) + H]+ was abundant in megakaryocytes, whereas [sphingomyelin(42:2) + H]+ was diminished in this cell type. These data reflect the vast molecular and cellular heterogeneity indicative of the bone marrow and the soft tissue surrounding the femur. Therefore, this application of multimodal MALDI IMS has the potential to advance bone-related biomedical research by offering deep molecular coverage in a preserved native bone microenvironment.

MALDI imaging mass spectrometry: an emerging tool in neurology

2021 ◽  
Author(s):  
Laura K. Schnackenberg ◽  
David A. Thorn ◽  
Dustyn Barnette ◽  
E. Ellen Jones
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

scholarly journals Region-specific bioconversion of dynorphin neuropeptide detected by in situ histochemistry and MALDI imaging mass spectrometry

Peptides ◽  
2017 ◽  
Vol 87 ◽  
pp. 20-27 ◽  
Author(s):  
Erik Bivehed ◽  
Robert Strömvall ◽  
Jonas Bergquist ◽  
Georgy Bakalkin ◽  
Malin Andersson
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Maldi Imaging Mass Spectrometry

MALDI Imaging Mass Spectrometry – A Novel Approach in Biomedical Research of Tissues

2013 ◽  
Vol 10 (2) ◽  
pp. 76-82
Author(s):  
Magdalena Kalinowska-Herok ◽  
Monika Pietrowska ◽  
Anna Walaszczyk ◽  
Piotr Widak
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Research ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Novel Approach ◽  
Maldi Imaging Mass Spectrometry
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