scholarly journals Dietary protein content and digestibility influences discrimination of amino acid nitrogen isotope values in a terrestrial omnivorous mammal

2021 ◽  
Vol 35 (11) ◽  
Author(s):  
John P. Whiteman ◽  
Mauriel Rodriguez Curras ◽  
Kelli L. Feeser ◽  
Seth D. Newsome
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Nitrogen Isotope ◽  
Amino Acid Nitrogen

scholarly journals Author Correction: Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuichi I. Naito ◽  
Ioana N. Meleg ◽  
Marius Robu ◽  
Marius Vlaicu ◽  
Dorothée G. Drucker ◽  
...  
Keyword(s):  
Amino Acid ◽  
Isotope Analysis ◽  
Nitrogen Isotope ◽  
Amino Acid Nitrogen ◽  
Nitrogen Isotope Analysis

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

1994 ◽  
Vol 72 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. Donkoh ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Crude Protein ◽  
Apparent Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Dietary Protein Level

The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

Effect of dietary protein level and salinity on growth, survival, enzymatic activities and amino-acid composition of the white shrimp Litopenaeus vannamei (Boone, 1931) juveniles

Crustaceana ◽  
2015 ◽  
Vol 88 (1) ◽  
pp. 82-95 ◽  
Author(s):  
Liying Sui ◽  
Guannan Ma ◽  
Yuangao Deng
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Muscle Tissue ◽  
Litopenaeus Vannamei ◽  
Dietary Protein ◽  
Protein Level ◽  
White Shrimp ◽  
Lower Growth ◽  
Protein Levels ◽  
Dietary Protein Level

Litopenaeus vannamei (Boone, 1931) postlarvae with average initial body weight of 0.089 g were reared in 75-litre PVC tanks for 40 days at salinities of 30 and 60 g l−1. The shrimps were fed compound feed containing protein levels of 35, 40, 45 and 50%, respectively. Salinity had a remarkable effect on growth and survival of L. vannamei juveniles. Higher survival rate and lower growth were observed at 60 g l−1 salinity. Dietary protein level affected the survival and growth of juveniles at both salinities, increased with dietary protein levels in the range of 35 to 45%, but decreased slightly with 50% dietary protein. Broken line analysis showed that the estimated optimal dietary protein levels at salinities of 30 and 60 g l−1 were 45.93 and 46.74%, respectively. Higher salinity resulted in an increased moisture content, ash and crude protein content in the shrimp muscle tissue. The amino acid contents in the shrimp muscle tissue were generally higher at 60 g l−1 salinity and increased dietary protein level led to higher protein content, except with 50% dietary protein. At salinity 60 g l−1, the soluble protein content and activities of glutamic oxalacetic transferase (GOT) and glutamic pyruvic transaminase (GPT) in shrimp muscle tissue were higher, while catalase (CAT) activities were lower. Farming of L. vannamei at a marginal culture salinity (60 g l−1) is feasible though the shrimps were likely exposed to stressful conditions. Reduced growth rate at higher salinity may be attributed to the higher total ammonium (TAN) concentration in the culture medium and extra energy consumption for osmoregulation at hypersaline conditions.

Amino Acid Oxidation Increases with Dietary Protein Content in Adult Neutered Male Cats as Measured Using [1-13C]Leucine and [15N2]Urea

2015 ◽  
Vol 145 (11) ◽  
pp. 2471-2478 ◽  
Author(s):  
Timothy J Wester ◽  
Karin Weidgraaf ◽  
Margreet Hekman ◽  
Claudia E Ugarte ◽  
Sandra F Forsyth ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Acid Oxidation ◽  
Amino Acid Oxidation

MON-P291: Dietary Protein Content Modulates the Amino-Acid and IGF1 Responses to Sucrose Overfeeding in Humans.

2017 ◽  
Vol 36 ◽  
pp. S285-S286 ◽  
Author(s):  
P. Jegatheesan ◽  
A. Surowska ◽  
V. Campos ◽  
J. Cros ◽  
N. Stefanoni ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein

Fasting affects amino acid nitrogen isotope values: a new tool for identifying nitrogen balance of free-ranging mammals

Oecologia ◽  
2020 ◽  
Vol 193 (1) ◽  
pp. 53-65 ◽  
Author(s):  
Nico Lübcker ◽  
John P. Whiteman ◽  
Robert P. Millar ◽  
P. J. Nico de Bruyn ◽  
Seth D. Newsome
Keyword(s):  
Amino Acid ◽  
Nitrogen Balance ◽  
Nitrogen Isotope ◽  
Amino Acid Nitrogen ◽  
Free Ranging

Influence of dietary protein of diets given in late gestation and during lactation on protein content and oestrogen concentrations in the colostrum and milk of gilts

1998 ◽  
Vol 67 (1) ◽  
pp. 139-145 ◽  
Author(s):  
H. Y. Al-Matubsi ◽  
G. Bervanakis ◽  
S. M. Tritton ◽  
R. G. Campbell ◽  
R. J. Fairclough
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Free Amino Acids ◽  
Free Amino ◽  
Post Partum ◽  
High Protein Diet ◽  
Late Gestation ◽  
Protein Diet

AbstractForty first cross hybrid gilts were used to study the effect of dietary protein during lactation on free amino acid, protein and total oestrogen concentrations in colostrum and milk. The diets were formulated to contain similar levels of digestible energy (DE) but with either 236 or 186 g protein per kg and 13·3 and 8·1 g lysine per kg air-dried diet respectively. Both diets were offered at 2·5 kg/day for 1 week prior to farrowing and ad libitum throughout a 24-day lactation (no. = 20 gilts per treatment). Colostrum and milk were collected manually at 3h (colostrum), 3, 7, 14 and 22 days (milk) post partum. Free amino acid concentrations in colostrum and milk were determined by high-performance liquid chromatography following derivatization of the amino acids by 9-fluorenylmethyl chloroformate. The lysine and glutamic acid concentrations in colostrum were the most abundant free amino acids followed by tryptophan and taurine in order of decreasing abundance. Milk free taurine concentrations increased to the greatest extent among all free amino acids over the 21 days of lactation. Gilts' mature milk was rich in free taurine, glutamic acid, glycine and lysine. During the first 3 h post partum, the protein content of colostrum was unaffected by dietary protein. However, milk protein content over 7 to 21 days of lactation was significantly higher in gilts offered the higher protein diet (P < 0·001). Total solids in colostrum and milk was not significantly affected by dietary protein. At 3h and 3 days post partum the concentrations of total oestrogen in colostrum and milk, determined by radioimmunoassay, were significantly higher in gilts offered the high protein diet (P < 0·05). These finding indicate that a high protein diet in late gestation and during lactation can influence protein content, amino acid and oestrogen concentrations of milk in gilts.

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