A mass spectrometry imaging method for visualizing synthetic polymers by using average molecular weight and dispersity as indices

2020 ◽  
Vol 34 (S2) ◽  
Author(s):  
Takaya Satoh ◽  
Sayaka Nakamura ◽  
Thierry Fouquet ◽  
Hiroaki Sato ◽  
Yoshihisa Ueda
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Mass Spectrometry Imaging ◽  
Average Molecular Weight ◽  
Synthetic Polymers ◽  
Imaging Method

scholarly journals A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

2018 ◽  
Vol 5 (11) ◽  
pp. 1800250 ◽  
Author(s):  
Jiuming He ◽  
Chenglong Sun ◽  
Tiegang Li ◽  
Zhigang Luo ◽  
Luojiao Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ambient Mass Spectrometry ◽  
Imaging Method

scholarly journals A straightforward electrospray ionization high resolution mass spectrometry method for underivatized long chain polysaccharides

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Roxana M. Ghiulai ◽  
Mirela Sarbu ◽  
Constantin Ilie ◽  
Alina D. Zamfir
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Electrospray Ionization ◽  
High Resolution Mass Spectrometry ◽  
Average Molecular Weight ◽  
Ionization Mass ◽  
Esi Ms ◽  
Singly Charged ◽  
Charged Ions ◽  
Long Chain

AbstractStructural analysis of long chain polysaccharides by electrospray ionization mass spectrometry (ESI-MS) is challenging since these molecules do not contain readily ionizable groups. Their mass spectra are dominated by singly charged ions, limiting the detection of high molecular weight species. Derivatization can enhance ionization, but analyte loss on purification decreases sensitivity. We report a method based on nanoESI-MS and MS/MS by collision induced dissociation (CID) for underivatized long chain polysaccharides. The procedure was tested on underivatized polydisperse dextrans (average molecular weight 4,000) at 2.6 kV ESI voltage and CID MS/MS at energies between 30-60 eV. 113 ions corresponding to species from Glc2 to Glc35 were detected. Ions at m/z 1,409.48, 1,107.35 and 1,438.47, assigned to [G17+2Na]2+,[G20+H+Na+K]3+ and [G35+2H+Na+K]4+, were sequenced and characterized by MS/MS. The component containing 35 Glc repeats is the longest polysaccharide chain detected by ESI-MS and structurally analyzed by MS/MS without prior derivatization and/or separation.

Structural units of maltenes and asphaltenes from hydrogenation of coal

1982 ◽  
Vol 47 (3) ◽  
pp. 843-852 ◽  
Author(s):  
Ivo Lang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Physical Meaning ◽  
Average Molecular Weight ◽  
Condensed System ◽  
Vapour Phase ◽  
Aromatic System ◽  
Structural Units ◽  
Unit Structure ◽  
Nmr Data

The calculation of the unit structure weight (USW) from NMR data is discussed. A comparison with the average molecular weight (Mr) values from vapour phase osmometry and mass spectrometry measurements showed that for the coal maltenes investigated (the fraction from the coal hydrogenation product soluble in pentane), USW can be looked upon as a rapid approximation of Mr. For asphaltenes, the calculation of USW is to a high extent dependent upon the assumed arrangement of the aromatic system. For an approximation of Mr of the asphaltenes studied, the most satisfactory was the calculation of USW from parameters of the Williams method assuming the occurrence of a peri-cata-condensed system. The USW of coal hydrogenation products calculated from NMR (1H and 13C) spectra should not be attributed the physical meaning of their smallest building units.

scholarly journals Quantitative immuno-mass spectrometry imaging of skeletal muscle dystrophin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
David P. Bishop ◽  
Mika T. Westerhausen ◽  
Florian Barthelemy ◽  
Thomas Lockwood ◽  
Nerida Cole ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Skeletal Muscle ◽  
Inductively Coupled Plasma ◽  
Mass Spectrometry Imaging ◽  
Gene Mutations ◽  
Drug Efficacy ◽  
Therapeutic Interventions ◽  
Imaging Method ◽  
Patient Resources ◽  
Viable Approach

AbstractEmerging and promising therapeutic interventions for Duchenne muscular dystrophy (DMD) are confounded by the challenges of quantifying dystrophin. Current approaches have poor precision, require large amounts of tissue, and are difficult to standardize. This paper presents an immuno-mass spectrometry imaging method using gadolinium (Gd)-labeled anti-dystrophin antibodies and laser ablation-inductively coupled plasma-mass spectrometry to simultaneously quantify and localize dystrophin in muscle sections. Gd is quantified as a proxy for the relative expression of dystrophin and was validated in murine and human skeletal muscle sections following k-means clustering segmentation, before application to DMD patients with different gene mutations where dystrophin expression was measured up to 100 µg kg−1 Gd. These results demonstrate that immuno-mass spectrometry imaging is a viable approach for pre-clinical to clinical research in DMD. It rapidly quantified relative dystrophin in single tissue sections, efficiently used valuable patient resources, and may provide information on drug efficacy for clinical translation.

scholarly journals A Systematic Study on the Degradation Products Generated from Artificially Aged Microplastics

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1997
Author(s):  
Greta Biale ◽  
Jacopo La Nasa ◽  
Marco Mattonai ◽  
Andrea Corti ◽  
Virginia Vinciguerra ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Degradation Products ◽  
Average Molecular Weight ◽  
Size Exclusion ◽  
Gas Chromatography Mass Spectrometry ◽  
Low Molecular Weight ◽  
Photo Degradation ◽  
Degradation Processes ◽  
Wide Range

Most of the analytical studies focused on microplastics (MPs) are based on the detection and identification of the polymers constituting the particles. On the other hand, plastic debris in the environment undergoes chemical and physical degradation processes leading not only to mechanical but also to molecular fragmentation quickly resulting in the formation of leachable, soluble and/or volatile degradation products that are released in the environment. We performed the analysis of reference MPs–polymer micropowders obtained by grinding a set of five polymer types down to final size in the 857–509 μm range, namely high- and low-density polyethylene, polystyrene (PS), polypropylene (PP), and polyethylene terephthalate (PET). The reference MPs were artificially aged in a solar-box to investigate their degradation processes by characterizing the aged (photo-oxidized) MPs and their low molecular weight and/or highly oxidized fraction. For this purpose, the artificially aged MPs were subjected to extraction in polar organic solvents, targeting selective recovery of the low molecular weight fractions generated during the artificial aging. Analysis of the extractable fractions and of the residues was carried out by a multi-technique approach combining evolved gas analysis–mass spectrometry (EGA–MS), pyrolysis–gas chromatography–mass spectrometry (Py–GC–MS), and size exclusion chromatography (SEC). The results provided information on the degradation products formed during accelerated aging. Up to 18 wt% of extractable, low molecular weight fraction was recovered from the photo-aged MPs, depending on the polymer type. The photo-degradation products of polyolefins (PE and PP) included a wide range of long chain alcohols, aldehydes, ketones, carboxylic acids, and hydroxy acids, as detected in the soluble fractions of aged samples. SEC analyses also showed a marked decrease in the average molecular weight of PP polymer chains, whereas cross-linking was observed in the case of PS. The most abundant low molecular weight photo-degradation products of PS were benzoic acid and 1,4-benzenedicarboxylic acid, while PET had the highest stability towards aging, as indicated by the modest generation of low molecular weight species.

Sign in / Sign up

Export Citation Format

Share Document