Dissociation of trivalent metal ion (Al3+, Ga3+, In3+and Rh3+)-peptide complexes under electron capture dissociation conditions

2016 ◽  
Vol 30 (6) ◽  
pp. 705-710 ◽  
Author(s):  
Xiangfeng Chen ◽  
Guoqiang Liu ◽  
Y. L. Elaine Wong ◽  
Liulin Deng ◽  
Ze Wang ◽  
...  
Keyword(s):  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Metal Ion ◽  
Trivalent Metal ◽  
Peptide Complexes

Electron-Capture Dissociation and Collision-Induced Dissociation of Lanthanide Metal–Ligand Complexes and Lanthanide Metal–Ligand Complexes Bound to Phosphopeptides

2009 ◽  
Vol 15 (2) ◽  
pp. 145-155 ◽  
Author(s):  
Jackie A. Mosely ◽  
Benjamin S. Murray ◽  
David Parker
Keyword(s):  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Metal Ion ◽  
Reduction Potential ◽  
Structural Information ◽  
Collision Induced Dissociation ◽  
Ion Cyclotron Resonance ◽  
Terbium Complex ◽  
Lanthanide Metal ◽  
Metal Ligand

Collision-induced dissociation (CID) and electron-capture dissociation (ECD) of lanthanide metal(III)–ligand complexes and lanthanide metal(III)–ligand–phosphopeptide complexes have been investigated using a Fourier transform-ion cyclotron resonance mass spectrometer (FT-ICR MS). Ternary adducts were formed for [LnL3+ + solvent anion(s) n–](3– n)+ [Ln = europium, terbium and ytterbium, L = heptadentate ligand, solvent anion = acetate or trifluoromethane-sulphonate (triflate)]. Results show that ECD provides much more diagnostically useful information than CID. ECD was found to systematically cleave N–C bonds in the “arms” of the ligand, similar to the N–Cα cleavage of peptides, generating two fragmentation sites per arm of the ligand. The most intense and informative fragment ions were obtained from the terbium complex and it is believed that this is a consequence of terbium's greater reduction potential: the greater the reduction potential, the greater the ligand fragmentation; the lower the reduction potential, the more likely the molecule is to relinquish the solvent anion. The choice of solvent is also shown to be important. In general, the complexes studied fragment easily to lose CH3CO2H; however, particularly for ECD, the complexes retain the triflate anion causing the ligand to fragment instead, thus providing much more useful information. The triflate anion can be displaced by a phosphopeptide to create a lanthanide metal–ligand–phosphopeptide adduct. ECD is able to sequence the phosphopeptide, locating the site of phosphorylation bound to [LnL]3+ and also confirm the identity of the ligand. Small differences in the fragmentation of the lanthanide metal–ligand–phosphopeptide adducts follow the trend Eu < Tb < Yb suggesting that charge density may now be a more significant factor than metal ion reduction potential. ECD analysis of the triflate salts of the terbium complexes is most informative in developing a method to optimise structural information that can be gained from this group of molecules by mass spectrometry.

scholarly journals Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry

2021 ◽  
Author(s):  
Kevin Jeanne Dit Fouque ◽  
Desmond Kaplan ◽  
Valery G. Voinov ◽  
Frederik H. V. Holck ◽  
Ole N. Jensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Trapped Ion
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