Silver stain removal using H2O2 for enhanced peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

2002 ◽  
Vol 16 (3) ◽  
pp. 160-168 ◽  
Author(s):  
Lloyd W. Sumner ◽  
Barbara Wolf-Sumner ◽  
Steven P. White ◽  
Victor S. Asirvatham
Keyword(s):  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Silver Stain ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Stain Removal ◽  
Mass Mapping ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Identification of Polypeptides Expressed in Response to Vinyl Chloride, Ethene, and Epoxyethane in Nocardioides sp. Strain JS614 by Using Peptide Mass Fingerprinting

2007 ◽  
Vol 73 (13) ◽  
pp. 4368-4372 ◽  
Author(s):  
Adina S. Chuang ◽  
Timothy E. Mattes
Keyword(s):  
Vinyl Chloride ◽  
Laser Desorption ◽  
Peptide Mass Fingerprinting ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Peptide Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.

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