A simple and rapid confirmatory assay for analyzing antibiotic residues of the macrolide class and lincomycin in bovine milk and yoghurt: hot water extraction followed by liquid chromatography/tandem mass spectrometry

2006 ◽  
Vol 21 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Sara Bogialli ◽  
Antonio Di Corcia ◽  
Aldo Laganà ◽  
Veronica Mastrantoni ◽  
Manuel Sergi
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Bovine Milk ◽  
Hot Water ◽  
Tandem Mass ◽  
Antibiotic Residues ◽  
Hot Water Extraction ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Confirmatory Assay

scholarly journals Screening and Quantitative Confirmatory Method for the Analysis of Glucocorticoids in Bovine Milk Using Liquid Chromatography-Tandem Mass Spectrometry

2010 ◽  
Vol 93 (5) ◽  
pp. 1656-1665 ◽  
Author(s):  
Edward M Malone ◽  
Chris T Elliott ◽  
David G Kennedy ◽  
Liam Regan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sodium Chloride ◽  
Bovine Milk ◽  
Clobetasol Propionate ◽  
Tandem Mass ◽  
Milk Samples ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Simultaneous Identification

Abstract An LC/MS/MS method was developed and validated for the simultaneous identification, confirmation, and quantification of 12 glucocorticoids in bovine milk. The method was validated in accordance with the criteria defined in Commission Decision 2002/657/EC. The developed method can detect and confirm the presence of dexamethasone, betamethasone, prednisolone, flumethasone, 6α-methylprednisolone, fluorometholone, triamcinolone acetonide, prednisone, cortisone, hydrocortisone, clobetasol propionate, and clobetasol butyrate in bovine milk. Milk samples are extracted with acetonitrile; sodium chloride is subsequently added to aid partition of the milk and acetonitrile mixture. The acetonitrile extract is then subjected to liquidliquid purification by the addition of hexane. The purified extract is evaporated to dryness and reconstituted in a wateracetonitrile mixture, and determination is carried out by LC/MS/MS. The method permits analysis of up to 30 samples in 1 day.

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