Brain-derived Neurotrophic Factor Signaling Mediates the Antidepressant-like Effect of the Total Flavonoids of Alpiniae Oxyphyllae Fructus in Chronic Unpredictable Mild Stress Mice

2016 ◽  
Vol 30 (9) ◽  
pp. 1493-1502 ◽  
Author(s):  
Tingxu Yan ◽  
Bo Wu ◽  
Zheng-zheng Liao ◽  
Bing Liu ◽  
Xu Zhao ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Brain Derived Neurotrophic Factor ◽  
Mild Stress ◽  
Total Flavonoids ◽  
Chronic Unpredictable Mild Stress ◽  
Neurotrophic Factor Signaling

scholarly journals Andrographolide Exerts Significant Antidepressant-Like Effects Involving the Hippocampal BDNF System in Mice

2019 ◽  
Vol 22 (9) ◽  
pp. 585-600 ◽  
Author(s):  
Jing-Jing Zhang ◽  
Ting-Ting Gao ◽  
Yuan Wang ◽  
Jin-Liang Wang ◽  
Wei Guan ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Forced Swim Test ◽  
Tail Suspension Test ◽  
Brain Derived Neurotrophic Factor ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Tail Suspension ◽  
Swim Test ◽  
Signalling Cascade ◽  
Suspension Test

Abstract Background Major depressive disorder is a worldwide neuropsychiatric disorder associated with various symptoms, but current antidepressants used in clinical practice have various side effects and high failure rates. Andrographolide is the main bioactive ingredient of Andrographis paniculata and exhibits numerous pharmacological actions. This study aimed to evaluate the antidepressant-like effects of andrographolide in male C57BL/6J mice. Methods The antidepressant-like effects of andrographolide in mice were explored in a forced swim test, tail suspension test, and chronic unpredictable mild stress model of depression. Western blotting and immunofluorescence were further performed to assess the effects of chronic unpredictable mild stress and andrographolide on the brain-derived neurotrophic factor signalling cascade and hippocampal neurogenesis. Moreover, a pharmacological inhibitor (K252a) and a lentiviral-short hairpin RNA (LV-TrkB-shRNA) were used to clarify the antidepressant-like mechanism of andrographolide. Results Andrographolide exhibited antidepressant-like potential in the forced swim test and tail suspension test without influencing the locomotor activity of mice. Repeated andrographolide treatment not only produced significant antidepressant-like effects in the chronic unpredictable mild stress model but also prevented the decreasing effects of chronic unpredictable mild stress on hippocampal brain-derived neurotrophic factor signalling and neurogenesis in mice. Importantly, blockade of the hippocampal brain-derived neurotrophic factor system by K252a and TrkB-shRNA fully abolished the antidepressant-like effects of andrographolide in mice. Conclusions Andrographolide exerts antidepressant-like effects in mice via promoting the hippocampal brain-derived neurotrophic factor signalling cascade.

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