Prevention of Hydrogen Peroxide-Induced Red Blood Cells Lysis by Ilex paraguariensis Aqueous Extract: Participation of Phenolic and Xanthine Compounds

2012 ◽  
Vol 27 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Ignacio N. Peralta ◽  
Laura Cogoi ◽  
Rosana Filip ◽  
Claudia Anesini
Keyword(s):  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Aqueous Extract ◽  
Blood Cells ◽  
Ilex Paraguariensis

scholarly journals An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System

Metabolites ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 175 ◽  
Author(s):  
Ioannis Tsamesidis ◽  
Chinedu O. Egwu ◽  
Pierre Pério ◽  
Jean-Michel Augereau ◽  
Françoise Benoit-Vical ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
High Resolution Mass Spectrometry ◽  
Modern Medicine ◽  
Reactive Species ◽  
Blood System ◽  
Pathological Conditions ◽  
Depth Study

Red blood cells are constantly exposed to reactive species under physiological or pathological conditions or during administration of xenobiotics. Regardless of the source, its accurate quantification is paramount in the area of theragnostics, which had been elusive up until now. Even if there are a lot of approaches to evaluate the oxidative stress, very sensitive methods are missing for the blood system. We therefore sought to apply a highly sensitive approach, by liquid chromatography coupled to mass spectrometry (UPLC–MS), for the quantification of reactive species such as superoxide radical and hydrogen peroxide using dihydroethidium (DHE) and coumarin boronic acid (CBA) probes respectively through the detection of 2-hydroxyethidium (2OH-E+) and 7-hydroxycoumarin (COH). The use of the high-resolution mass spectrometry associated to UPLC ensured a selective detection of superoxide and hydrogen peroxide in the blood system under diverse conditions such as oxidized red blood cells (RBCs), untreated and treated parasitized RBCs. Moreover, this technique allowed the determination of reactive species in human plasma. This protocol provides a huge opportunity for in-depth study of several pathological conditions vis-a-vis their treatment in modern medicine.

Hydrogen peroxide production by red blood cells

1994 ◽  
Vol 16 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Cecilia Giulivi ◽  
Paul Hochstein ◽  
Kelvin J.A. Davies
Keyword(s):  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hydrogen Peroxide Production

Mercury vapor uptake and hydrogen peroxide detoxification in human and mouse red blood cells

1988 ◽  
Vol 96 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Stefan Halbach ◽  
Nazzareno Ballatori ◽  
Thomas W. Clarkson
Keyword(s):  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Mercury Vapor ◽  
Human And Mouse

scholarly journals THE ETHYL ACETATE FRACTION OF PSOROSPERMUM FEBRIFUGUM SPACHROOT BARKS AQUEOUS EXTRACT IS A GOOD STIMULATOR OF HEMATOPOIESIS

2020 ◽  
Vol 8 (10) ◽  
pp. 1018-1026
Author(s):  
Maximin Senou ◽  
◽  
Pascal Atchade Tchogou ◽  
Felicienne Agbogba ◽  
Ezeckiel Jacques Lokonon ◽  
...  
Keyword(s):  
Blood Cell ◽  
Ethyl Acetate ◽  
Red Blood Cells ◽  
Aqueous Extract ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Extract Fraction ◽  
Dose Dependent

PsorospermumfebrifugumSpach. (Clusiaceae) is a medicinal plant found in Benin whose root bark was effective in treating anemia. To identify the family of chemical compounds of this organ was responsible for its hematopoietic efficiency, this work aimed to test the ethyl acetate fraction of the aqueous extract of the plant organ on anemic rats. Methods: Wistar rats were anemic by phenylhydrazinechloridrate on D0. From day 2 to day 15, some were gavage fed with the ethyl acetate fraction of PsorospermumfebrifugumSpach root bark aqueous extract at 40 or 60 mg / kg / day. Others received either vitafer as a reference drug or distilled water (untreated anemic group). Blood samples were collected from these rats and non-anemic control rats at days 0, 2, 7, 10 and 15 for the blood count and osmotic resistance of red blood cells. Results: At D2, phenylhydrazine significantly decreased hemoglobin and red blood cell number, which were corrected on D7 by the extract fraction with a dose-dependent effect. The extract fraction rapidly stimulated release of macrocytes, immature red blood cells in the first week to compensate the anemia. The extract did not affect blood platelet number, suggesting some specificity of action on the red blood cell line. Conclusion: The ethyl acetate fraction of Psorospermumfebrifugum root bark aqueous extract stimulated erythropoiesis faster than the crude extract. Its action seemed specific and dose-dependent. It would probably be related to the flavonoids which action mechanism needs to be explored.

scholarly journals Efficacy of Butanolic Fraction of Cocos Nucifera's Root Aqueous Extract on Induced Anemia Treatment

2021 ◽  
Vol 13 (2) ◽  
pp. 49
Author(s):  
Tchogou AP ◽  
Sènou M ◽  
Agbogba F ◽  
Lokonon JE ◽  
Medoatinsa SE ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Aqueous Extract ◽  
Blood Cells ◽  
Complete Blood Count ◽  
Cocos Nucifera ◽  
Coastal Plant ◽  
Dose Dependent ◽  
Anemia Treatment ◽  
Stimulation Of

Cocos nucifera was a coastal plant whose roots were used in pharmacopoeia to treat anemia in Benin. The aqueous extract from its roots stimulated the synthesis of hemoglobin. The aim of this work was to test in vivo the efficacy of the butanolic fraction of the extract in the treatment of anemia. Methods: Wistar rats were anemic with phenylhydrazine for two days. From D2 to D15, some were treated by gavage with the butanolic fraction of the aqueous extract of Cocos nucifera roots at the dose of 40 mg or 60 mg / kg of body weight / day, others were treated with vitafer (an anti-anemic drug) or with distilled water. The rats blood were collected on days D0, D2, D7, D10 and D15 for the complete blood count and the osmotic resistance of the red blood cells. Results: On D2, phenylhydrazine significantly lowered the hemoglobin level and the number of red blood cells, which were respectively corrected on D10 and D15 by the fraction of extract with release of hypochromic macrocytes. However, the effect was slower than that of the crude extract, was not specific to erythropoiesis because it also stimulated thrombopoiesis and was not dose-dependent. Conclusion: The butanolic fraction of the aqueous extract of Cocos nucifera roots corrected anemia by stimulation of hematopoiesis. The observed biological activity would probably be linked to anthocyanins which are mainly isolated by butanol. These results contribute to a better knowledge of bioactive compounds of our antianemic plants.

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