In vivo inhibitory effects of arjunolic acid derivatives on two-stage carcinogenesis in mouse skin

1995 ◽  
Vol 9 (6) ◽  
pp. 444-447 ◽  
Author(s):  
B. Diallo ◽  
R. Vanhaelen-Fastré ◽  
M. Vanhaelen ◽  
T. Konoshima ◽  
M. Takasaki ◽  
...  
Keyword(s):  
Mouse Skin ◽  
Inhibitory Effects ◽  
Two Stage ◽  
Arjunolic Acid

scholarly journals Inhibitory Effects of Gymnema (Gymnema sylvestre) Leaves on Tumour Promotion in Two-Stage Mouse Skin Carcinogenesis

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ken Yasukawa ◽  
Sakiko Okuda ◽  
Yasuhito Nobushi
Keyword(s):  
Mouse Skin ◽  
Ethanol Extract ◽  
Skin Carcinogenesis ◽  
Gymnema Sylvestre ◽  
Active Fraction ◽  
Inhibitory Effects ◽  
Two Stage ◽  
Tumour Promotion ◽  
Ethanol Extracts

Ethanol extracts of gymnema (Gymnema sylvestre) leaves exhibited marked antitumour-promoting activity in anin vivotwo-stage carcinogenesis test in mice using 7,12-dimethylbenz[a]anthracene as an initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoter. From the active fraction of the ethanol extract of the gymnema leaves, three triterpenoids were isolated and identified. These compounds were evaluated for their inhibitory effects on TPA-induced inflammation (1 µg/ear) in mice. The tested compounds showed marked anti-inflammatory effects, with a 50% inhibitory dose of 50–555 nmol/ear.

Annonacin Suppresses Tumor Promoting Stage in Two Stage Mouse Skin Tumorigenesis via Modulation of Multiple Cancer Signaling Pathways

2018 ◽  
Author(s):  
Mohd Rohaizad Md Roduan ◽  
Norhafizah Mohtarrudin ◽  
Roslida Abd Hamid
Keyword(s):  
Signaling Pathways ◽  
Histopathological Examination ◽  
Mouse Skin ◽  
Latency Period ◽  
Multiple Cancer ◽  
Two Stage ◽  
Skin Tumorigenesis ◽  
Cancer Signaling ◽  
Liver And Kidney

Annonacin, an annonaceous acetogenin isolated from Annona muricata has been reported to be strongly cytotoxic against various cell lines, in vitro. Nevertheless, its effect against in vivo tumor promoting activity has not been reported yet. Therefore, this study was aimed to investigate antitumor-promoting activity of annonacin via in vivo two-stage mouse skin tumorigenesis model and its molecular pathways involved. Annonacin 85 nM was topically applied to DMBA/TPA-induced ICR mice for 22 weeks of treatment. Histopathological examination of skin, liver and kidney as well as genes and proteins expression analysis were conducted. Annonacin significantly increased the tumor latency period and also reduced the tumor incidence, tumor burden and tumor volume, respectively. In addition, it also suppressed tumorigenesis manifested by significant reduction of hyperkeratosis, dermal papillae and number of keratin pearls on skin tissues. Annonacin also appeared to be non-toxic to liver and kidney. Significant modulation of both AKT, ERK, MTOR, p38, PTEN and Src genes and proteins were also observed in annonacin-targeted signaling pathway(s) against tumorigenesis. Collectively, results of this study indicate that annonacin is a potential therapeutic compound targeting tumor promoting stage in skin tumorigenesis by modulating multiple gene and protein in cancer signaling pathways without apparent toxicity.

Inhibitory Effects of Lanostane-Type Triterpene Acids, the Components of Poria cocos, on Tumor Promotion by 12-O-Tetradecanoylphorbol-13-Acetate in Two-Stage Carcinogenesis in Mouse Skin

Oncology ◽  
1996 ◽  
Vol 53 (5) ◽  
pp. 382-385 ◽  
Author(s):  
Tomohiro Kaminaga ◽  
Ken Yasukawa ◽  
Hiroshi Kanno ◽  
Takaaki Tai ◽  
Yoshiki Nunoura ◽  
...  
Keyword(s):  
Mouse Skin ◽  
Tumor Promotion ◽  
Inhibitory Effects ◽  
Two Stage ◽  
Poria Cocos ◽  
Triterpene Acids

Cancer-chemopreventive effects of natural sweeteners and related compounds

2002 ◽  
Vol 74 (7) ◽  
pp. 1309-1316 ◽  
Author(s):  
Takao Konoshima ◽  
Midori Takasaki
Keyword(s):  
Mouse Skin ◽  
Stevia Rebaudiana ◽  
Chemical Carcinogenesis ◽  
Inhibitory Effect ◽  
Skin Carcinogenesis ◽  
Inhibitory Effects ◽  
Chemopreventive Agents ◽  
Vitro Assay

To search for possible cancer-chemopreventive agents from natural resources, several natural sweeteners were screened by the in vitro assay indicated by the inhibitory effects of Epstein-Barr virus early antigen (EBV-EA) induction. Of active compounds that showed the remarkable inhibitory effects on the EBV-EA induction, stevioside, from the leaves of Stevia rebaudiana, and mogroside V, from the fruits of Momordica grosvenori, exhibited significant inhibitory effects on the two-stage mouse skin carcinogenesis in vivo induced by 7,12-dimethylbenz[a]anthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA). The inhibitory effect of stevioside is stronger than that of glycyrrhizin, which had been known as an antitumor-promoter in chemical carcinogenesis. Furthermore, stevioside also inhibited mouse skin carcinogenesis initiated by peroxynitrite. These results suggest that stevioside and mogroside V might be valuable as chemopreventive agents for chemical carcinogenesis.

Sign in / Sign up

Export Citation Format

Share Document