Euphytose®, an association of plant extracts with anxiolytic activity: Investigation of its mechanism of action by anin vitro binding study

1991 ◽  
Vol 5 (6) ◽  
pp. 241-244 ◽  
Author(s):  
Marc Valli ◽  
Monique Paubert-Braquet ◽  
Sylvie Picot ◽  
Richard Fabre ◽  
Gérard Lefrançois ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Plant Extracts ◽  
Anxiolytic Activity ◽  
Binding Study ◽  
Vitro Binding

scholarly journals Effect of ethanol and cocaine on [11C]MPC-6827 uptake in SH-SY5Y cells

2021 ◽  
Author(s):  
Naresh Damuka ◽  
Miranda Orr ◽  
Paul W. Czoty ◽  
Jeffrey L. Weiner ◽  
Thomas J. Martin ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Ex Vivo ◽  
Neuroblastoma Cells ◽  
Proper Function ◽  
Brain Functions ◽  
Biodistribution Studies ◽  
Positron Emission ◽  
Vitro Binding

AbstractMicrotubules (MTs) are structural units in the cytoskeleton. In brain cells they are responsible for axonal transport, information processing, and signaling mechanisms. Proper function of these processes is critical for healthy brain functions. Alcohol and substance use disorders (AUD/SUDs) affects the function and organization of MTs in the brain, making them a potential neuroimaging marker to study the resulting impairment of overall neurobehavioral and cognitive processes. Our lab reported the first brain-penetrant MT-tracking Positron Emission Tomography (PET) ligand [11C]MPC-6827 and demonstrated its in vivo utility in rodents and non-human primates. To further explore the in vivo imaging potential of [11C]MPC-6827, we need to investigate its mechanism of action. Here, we report preliminary in vitro binding results in SH-SY5Y neuroblastoma cells exposed to ethanol (EtOH) or cocaine in combination with multiple agents that alter MT stability. EtOH and cocaine treatments increased MT stability and decreased free tubulin monomers. Our initial cell-binding assay demonstrated that [11C]MPC-6827 may have high affinity to free/unbound tubulin units. Consistent with this mechanism of action, we observed lower [11C]MPC-6827 uptake in SH-SY5Y cells after EtOH and cocaine treatments (e.g., fewer free tubulin units). We are currently performing in vivo PET imaging and ex vivo biodistribution studies in rodent and nonhuman primate models of AUD and SUDs and Alzheimer's disease.

Anxiolytic Activity of Apocynum venetum L. and its Proposed Mechanism of Action

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
O Grundmann ◽  
J Nakajima ◽  
V Butterweck
Keyword(s):  
Mechanism Of Action ◽  
Anxiolytic Activity ◽  
Apocynum Venetum

Further evidence for the presence of androgen receptors in the lungs and in the head appendages of the cock

1977 ◽  
Vol 55 (3) ◽  
pp. 263-265 ◽  
Author(s):  
Jean Y. Dubé ◽  
Pierre Chapdelaine ◽  
Roland R. Tremblay
Keyword(s):  
Density Gradient ◽  
Mechanism Of Action ◽  
Androgen Receptors ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sucrose Density Gradient ◽  
Sucrose Density Gradient Centrifugation ◽  
In Vitro Binding ◽  
Vitro Binding

The presence of cytoplasmic dihydrotestosterone receptors in the lungs, the comb, the wattle, and the ear lobes of the cock was demonstrated by sucrose density-gradient centrifugation. All these tissues exhibited saturable 'in vitro' binding of dihydrotestosterone in the 8–11S region of the gradient. When 0.5 M KCl was added to the lung, wattle, and comb cytosols and to the gradients, the radioactive dihydrotestosterone migrated in the 4–5S region. These studies suggest that the mechanism of action of androgens in the head appendages of the cock and in other target tissues is similar.

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