In vitro cytotoxicity testing of natural products using guinea-pig ear keratinocytes

1988 ◽  
Vol 2 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Ann Harris ◽  
Alison T. Keene ◽  
J. David Phillipson ◽  
David C. Warhurst
Keyword(s):  
Natural Products ◽  
Guinea Pig ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Testing

Erratum to “In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines” [Toxicology in Vitro 18 (2004) 365–376]

2004 ◽  
Vol 18 (5) ◽  
pp. 731
Author(s):  
S Nı́ Shúilleabháin ◽  
C Mothersill ◽  
D Sheehan ◽  
N.M O'Brien ◽  
J O'Halloran ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Metal Salts ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Zinc Metal ◽  
Cytotoxicity Testing

In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media

2005 ◽  
Vol 21 (5-6) ◽  
pp. 147-154 ◽  
Author(s):  
S Bakand ◽  
A Hayes ◽  
C Winder ◽  
C Khalil ◽  
B Markovic
Keyword(s):  
Culture Media ◽  
In Vitro Cytotoxicity ◽  
Serum Free ◽  
Cytotoxicity Testing

Cytotoxic and Apoptotic Action of Nonactin and Cephaeline. HBr Precludes Alteration of Redox Status

2019 ◽  
Vol 9 (4) ◽  
pp. 295-302
Author(s):  
Oluyomi Stephen Adeyemi ◽  
Oluwakemi Josephine Awakan ◽  
Anne Adebukola Adeyanju ◽  
David Adeiza Otohinoyi
Keyword(s):  
Natural Products ◽  
Mammalian Cells ◽  
Redox Status ◽  
In Vitro Cytotoxicity ◽  
Ros Production ◽  
Cellular Toxicity ◽  
Leading Role ◽  
Show Evidence ◽  
Cellular Apoptosis

Background: Natural products or naturally derived compounds are invaluable to human and animal lives either for nutritional value or for medicinal purposes. Indeed, natural products including extracts containing polychemical mixtures play a leading role in the discovery and development of drugs. However, the increasing interest in natural medicines is also attracting a growing concern about the safety of naturally derived medications. Objective: In the present study, we evaluated several naturally derived compounds for in vitro cytotoxicity in mammalian cells. Methods: A total of 54 compounds were evaluated for in vitro cytotoxic and apoptotic action in Human Fibroblast Foreskin (HFF) cells. Results: Of the 54 natural compounds screened for cellular toxicity, only nonactin and cephaeline. HBr reduced cellular viability by ≥60% with IC50 value <3 µg/ml. Addition of trolox antioxidant to the assay medium failed to abate cellular toxicity by both nonactin and cephaeline.HBr treatments. Fluorescence evaluation for Reactive Oxygen Species (ROS) production as well as Mitochondrial Membrane Potential (MMP) was negative for both nonactin and cephaeline.HBr treatments. In contrast, both nonactin and cephaeline.HBr caused cellular apoptosis and this was not attenuated even in the presence of trolox. Conclusion: Taken together, we show evidence supporting that cytotoxic and apoptotic action of nonactin and cephaeline.HBr precludes oxidative stress or ROS production.

scholarly journals Study of the in vitro cytotoxicity testing of medical devices

2015 ◽  
Vol 3 (5) ◽  
pp. 617-620 ◽  
Author(s):  
WEIJIA LI ◽  
JING ZHOU ◽  
YUYIN XU
Keyword(s):  
Medical Devices ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Testing

In vitro cytotoxicity testing of three zinc metal salts using established fish cell lines

2004 ◽  
Vol 18 (3) ◽  
pp. 365-376 ◽  
Author(s):  
S Nı́ Shúilleabháin ◽  
C Mothersill ◽  
D Sheehan ◽  
N.M O’Brien ◽  
J O’ Halloran ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Metal Salts ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Zinc Metal ◽  
Cytotoxicity Testing

scholarly journals National Cancer Institute pediatric preclinical testing program: Model description for in vitro cytotoxicity testing

2010 ◽  
Vol 56 (2) ◽  
pp. 239-249 ◽  
Author(s):  
Min H. Kang ◽  
Malcolm A. Smith ◽  
Christopher L. Morton ◽  
Nino Keshelava ◽  
Peter J. Houghton ◽  
...  
Keyword(s):  
National Cancer Institute ◽  
In Vitro Cytotoxicity ◽  
Model Description ◽  
Program Model ◽  
Preclinical Testing ◽  
Testing Program ◽  
Cytotoxicity Testing

scholarly journals In vitro antimicrobial and cytotoxic activity of Lippia origanoides essential oil against bacteria of potential health concern

2021 ◽  
Author(s):  
Edwin Stiven Quiguanás Guarín ◽  
Juan Pablo Bedoya Agudelo ◽  
Jhon Esteban Lopez-Carvajal ◽  
Yuly Andrea Ramírez Tabares ◽  
Leonardo Padilla Sanabria ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Natural Products ◽  
Essential Oil ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Health Concern ◽  
Chemical Components ◽  
Potential Health ◽  
Major Chemical

Due to the growing resistance they develop of bacteria to drugs, the search for alternatives in natural products is considered important such as Lippia origanoides essential oil. Here, the antibacterial activity of the oil and two of its major chemical components were tested against bacteria of potential health concern. The cytotoxicity of these compounds was evaluated in human erythrocytes and Vero cells. 51 compounds were identified in the LOEO, being terpinen-4-ol, γ-Terpinene, citronellal and thymol the main. LOEO and thymol showed antibacterial activity from 904 μg/mL and 200 μg/mL, respectively. γ-Terpinene did not show activity any concentration tested. LOEO showed hemolysis at concentration of 3000 μg/mL and thymol at 100 μg/mL. LOEO and thymol showed cytotoxicity in the evaluated cell lines at 250 μg/mL and 100 μg/mL, respectively. These compounds have a moderate cytotoxicity so it's considered necessary to study alternatives to reduce the in vitro cytotoxicity of these compounds.

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