The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability

2021 ◽  
Author(s):  
Mostafa Ghasemisarabbadieh ◽  
Sveinbjörn Gizurarson ◽  
Benjamín Ragnar Sveinbjörnsson
Keyword(s):  
Acetate Buffer ◽  
Buffer Concentration

scholarly journals The effects of acetate buffer concentration on lysozyme solubility

1996 ◽  
Vol 168 (1-4) ◽  
pp. 112-117 ◽  
Author(s):  
Elizabeth L. Forsythe ◽  
Marc L. Pusey
Keyword(s):  
Acetate Buffer ◽  
Buffer Concentration

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

Separation of Sucralose in Food Samples using Amines as Background Electrolyte Supported with DFT Calculations

2020 ◽  
Vol 16 ◽  
Author(s):  
Bathinapatla Ayyappa ◽  
Suvardhan Kanchi ◽  
Myalowenkosi I. Sabela ◽  
Krishna Bisetty
Keyword(s):  
Background Electrolyte ◽  
Food Additives ◽  
Daily Intake ◽  
Food Samples ◽  
Uv Detection ◽  
Migration Time ◽  
Artificial Sweetener ◽  
Buffer Concentration ◽  
Electrophoretic Method ◽  
Amine Buffers

BACKGROUND: Sucralose is a high intensity artificial sweetener sucralose and chemically known as 1,6-dichloro-1,6-dideoxy-β-D-fructofuranosyl-4-chloro-4-deoxy-α-D-galactopyranoside. It is used as a sweetener and flavour enhancer in foods and beverages. Due to its high stability at wider temperatures and pH, made its applicability in various food products throughout the world. As per Joint FAO/WHO Expert Group on Food Additives (JECFA) in 1990, the daily intake of sucralose is 0-15 mg/kg body weight. The literature reports suggest that sucralose has a possible health threat due to the presence of chlorine groups, thereby leading to the several illnesses. The growing interest on the use of SUC in the foods, makes it necessary in developing a fast, reliable, cost effective and reproducible analytical method to determine SUC in food samples. The detection of sucralose and other carbohydrates like fructose, glucose and sucrose is a challenging task owing to its: (i) unavailability of the charged functions and (ii) lack of absorption of strong chromophoric nature in the UV region. Therefore, separation of non-absorbing neutral molecules needs a careful procedure with the suitable electrolyte systems. METHODOLOGY: An indirect UV detection capillary electrophoretic method is described for the separation of sucralose in different food samples. It was achieved by nucleophile substitution (SN2) in the presence of amine as background electrolytes. The morpholine buffer showed good buffering capacity in terms of migration time (< 8.0 min) and baseline stability when compared to other amine buffers (ethylamine, piperidine, triethylamine). The analytical applications of proposed method showed by recovery percentages of sucralose in real and spiked samples on intra and inter-day basis at optimum experimental conditions of 0.2 M buffer concentration and pH 12.0 at 230 nm UV detection. RESULTS: The selection of BGE, UV detection wavelength, buffer concentration, buffer pH, cassette temperature and applied voltage were optimized to enhance the sensitivity and selectivity of the separation method. Recoveries obtained were ranging from 96.87 to 98.82 % for real samples and 94.45 to 98.06 % for spiked samples respectively. Linearity was studied in the range of 2-10 mM, and showed a correlation coefficients of 0.9942 with LOD and LOQ found to be 0.3804 mg L-1 and 1.5215 mg L-1 with % RSD (n = 5) ± 1.27 and 1.19 % with respect to migration time and peak area. Furthermore, to better understand the separation of sucralose with amine buffers were investigated computationally using HOMO-LUMO calculations. The obtained results showed that the band gap decreases in the presence of amine moiety irrespective of its nature. CONCLUSION: In the study, novel background electrolytic system was successfully applied to separate sucralose using indirect UV detector with capillary electrophoresis. The FT-IR results confirmed that the interaction of sucralose with different amine buffers to better understand the separation chemistry behind sucralose and amine complexes. Moreover, computational results indicate that the direction of charge transfer from the amine functionality to the glucofuranosyl ring in each amine derivative of sucralose confirms the strong interaction between sucralose and amines, which led in the baseline separation of sucralose in different food samples.

Electron Microprobe Analyses of the Remineralization of Enamel

1970 ◽  
Vol 49 (3) ◽  
pp. 621-625 ◽  
Author(s):  
Stephen H.Y. Wei
Keyword(s):  
Acetic Acid ◽  
Electron Microprobe ◽  
Potassium Acetate ◽  
Enamel Surface ◽  
Acetate Buffer ◽  
Demineralized Enamel ◽  
Calcium And Phosphorus

The electron microprobe was used to analyze the calcium and phosphorus concentrations of sound, acid-etched, and remineralized enamel. By use of a weak acetic acid-potassium acetate buffer, it was found that the demineralization probably affected only the first 10 micrometers of the enamel surface. This demineralized enamel was successfully remineralized by the use of a calcifying solution. The changes in calcium and phosphorus concentrations and the Ca/P ratios were determined.

Enzymatic hydrolysis of chitosan in acetate buffer solution

2015 ◽  
Vol 88 (4) ◽  
pp. 647-651 ◽  
Author(s):  
V. V. Chernova ◽  
A. S. Shurshina ◽  
M. V. Bazunova ◽  
E. I. Kulish
Keyword(s):  
Enzymatic Hydrolysis ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Hydrolysis Of

Polarographic behaviour of zinc in acetate buffer

1962 ◽  
Vol 50 (6) ◽  
pp. 1032-1034 ◽  
Author(s):  
O. P. Agrawal ◽  
R. C. Kapoor
Keyword(s):  
Acetate Buffer ◽  
Polarographic Behaviour

Molecular Imprinted Graphene Oxide Nanocomposite for Optical Sensing of Nicotine in Human Blood Plasma

2020 ◽  
Vol 42 (6) ◽  
pp. 856-856
Author(s):  
Sehrish Qazi Sehrish Qazi ◽  
Huma Shaikh Huma Shaikh ◽  
Ayaz Ali Memon Ayaz Ali Memon ◽  
and Shahabuddin Memon and Shahabuddin Memon
Keyword(s):  
Graphene Oxide ◽  
Human Plasma ◽  
Human Blood ◽  
Optical Sensor ◽  
Single Layer ◽  
Human Blood Plasma ◽  
Buffer Concentration ◽  
Imprinted Polymer ◽  
Ft Ir

Among all psychotropic alkaloids, nicotine is more addictive, carcinogenic and capable of causing many health problems. This work is based on the development of highly robust, cheap, reliable, selective and sensitive nicotine imprinted graphene oxide nanocomposite (ImpGO nanocomposite) based optical sensor for determination of nicotine in human plasma. The ImpGO nanocomposite has been thoroughly characterized using different techniques i.e. FT-IR, SEM, TEM, Raman, etc. These characterizations revealed that ImpGO nanocomposite is comprised of single layer of graphene oxide successfully modified with imprinted polymer. The synthesized material was utilized to selectively determine nicotine using UV-vis spectrophotometer in BR buffer of 0.1 M at pH 3 and diluted human plasma. The effect of parameters such as buffer concentration, pH, amount of nanocomposite, etc on determination of nicotine using ImpGO nanocomposite were studied thoroughly. Thus, a sensitive optical method was developed for determination of nicotine in human plasma with linear range of 22-370 pM along with LOD and LOQ of 7 pM and 22 pM, respectively. The selectivity of sensor was evaluated using homologues of nicotine such as nicotine amide, caffeine and cotinine. The results obtained from biological samples showed that developed optical sensor is efficient in complex matrices of real sample.

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