Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptide

2009 ◽  
Vol 15 (9) ◽  
pp. 589-594 ◽  
Author(s):  
Ramamourthy Gopal ◽  
Seong-Cheol Park ◽  
Kyeoung-Ju Ha ◽  
Seung Joo Cho ◽  
Si Wouk Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Analog Peptide ◽  
Lysine Substitution

Structure-guided de novo design of α-helical antimicrobial peptide with enhanced specificity

2010 ◽  
Vol 82 (1) ◽  
pp. 243-257 ◽  
Author(s):  
Jin-Feng Huang ◽  
Yi-Min Xu ◽  
Dian-Ming Hao ◽  
Yi-Bing Huang ◽  
Yu Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Hemolytic Activity ◽  
De Novo ◽  
Biological Activities ◽  
Peptide Sequence ◽  
Rational Approach ◽  
Peptide Antibiotics ◽  
Self Association ◽  
Lysine Substitution

In the present study, the 26-residue peptide sequence Ac-KWKSFLKTFKSAKKTVLHTALKAISS-amide (peptide P) was utilized as the framework to study the effects of introducing hydrophilic amino acid lysine on the nonpolar face of the helix on peptide biological activities. Lysine residue was systematically used to substitute original hydrophobic amino acid at the selected locations on the nonpolar face of peptide P. In order to compensate for the loss of hydrophobicity caused by lysine substitution, leucine was also used to replace original alanine to increase peptide overall hydrophobicity. Hemolytic activity is correlated with peptide hydrophobicity. By introducing lysine on the nonpolar face, we significantly weaken peptide hemolytic activity as well as antimicrobial activity. However, by utilizing leucine to compensate the hydrophobicity, we improve antimicrobial activity against both Gram-negative and -positive bacteria. Peptide self-association ability and hydrophobicity were also determined. This specific rational approach of peptide design could be a powerful method to optimize antimicrobial peptides with clinical potential as peptide antibiotics.

scholarly journals Structure-activity relationship of HP (2–20) analog peptide: Enhanced antimicrobial activity by N-terminal random coil region deletion

Biopolymers ◽  
2007 ◽  
Vol 88 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Yoonkyung Park ◽  
Seong-Cheol Park ◽  
Hae-Kyun Park ◽  
Song Yub Shin ◽  
Yangmee Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Structure Activity Relationship ◽  
Random Coil ◽  
Activity Relationship ◽  
Structure Activity ◽  
Analog Peptide ◽  
Relationship Of

Antimicrobial activity of withanolides from the leaves of Withania aristata

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
L Araujo ◽  
N Padilla ◽  
GG Llanos ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antimicrobial Activity

Antimicrobial activity of Eurycoma longifolia and Momordica charantia

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
R Łos ◽  
K Skalicka-Wozniak ◽  
J Widelski ◽  
A Malm ◽  
K Głowniak
Keyword(s):  
Antimicrobial Activity ◽  
Momordica Charantia ◽  
Eurycoma Longifolia

Antioxidant and antimicrobial activity of willow herb (Epilobium angustifolium L.)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak
Keyword(s):  
Antimicrobial Activity ◽  
Epilobium Angustifolium ◽  
Antioxidant And Antimicrobial Activity

Cytotoxicity, antimicrobial activity and composition of essential oil from Tanacetum balsamita L.subsp. balsamita

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
SN Ebrahimi ◽  
M Yousefzadi ◽  
A Sonboli ◽  
F Miraghasi ◽  
S Ghiasi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil
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