Helicoverpa armigera and Helicoverpa zea hybridization: constraints, heterosis, and implications for pest management

2021 ◽  
Author(s):  
Danielly A M Rios ◽  
Alexandre Specht ◽  
Vânia F Roque‐Specht ◽  
Daniel R Sosa‐Gómez ◽  
Júlia Fochezato ◽  
...  
Keyword(s):  
Pest Management ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Helicoverpa Armígera

scholarly journals Common Receptor for Bacillus thuringiensis Toxins Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua

2005 ◽  
Vol 71 (9) ◽  
pp. 5627-5629 ◽  
Author(s):  
Carmen Sara Hernández ◽  
Juan Ferré
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Spodoptera Exigua ◽  
Cross Resistance ◽  
Binding Studies ◽  
Lepidopteran Species ◽  
Common Receptor ◽  
Binding Data ◽  
Helicoverpa Armígera

ABSTRACT Binding studies using 125I-Cry1Ac and biotinylated Cry1Fa toxins indicate the occurrence of a common receptor for Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua. Our results, along with previous binding data and the observed cases of cross-resistance, suggest that this pattern seems to be widespread among lepidopteran species.

scholarly journals Phylogeography of the Recent Expansion of Helicoverpa armigera (Lepidoptera: Noctuidae) in South America and the Caribbean Basin

2019 ◽  
Vol 112 (4) ◽  
pp. 388-401 ◽  
Author(s):  
Luke R Tembrock ◽  
Alicia E Timm ◽  
Frida A Zink ◽  
Todd M Gilligan
Keyword(s):  
South America ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
New World ◽  
The United States ◽  
Caribbean Basin ◽  
Agricultural Pests ◽  
Multiple Introductions ◽  
Helicoverpa Armígera ◽  
The Caribbean

Abstract The Old World bollworm, Helicoverpa armigera (Hübner), is one of the most destructive agricultural pests worldwide. It was first recorded in Brazil in 2013, yet despite this recent introduction, H. armigera has spread throughout much of Latin America. Where H. armigera has become established, it is displacing or hybridizing with the congeneric New World pest Helicoverpa zea. In addition to the adaptive qualities that make H. armigera a megapest, such as broad range pesticide resistance, the spread of H. armigera in the New World may have been hastened by multiple introductions into South America and/or the Caribbean. The recent expansion of the range of H. armigera into the New World is analyzed herein using mtDNA of samples from South America, the Caribbean Basin, and the Florida Peninsula. Phylogeographic analyses reveal that several haplotypes are nearly ubiquitous throughout the New World and native range of H. armigera, but several haplotypes have limited geographic distribution from which a secondary introduction with Euro-African origins into the New World is inferred. In addition, host–haplotype correlations were analyzed to see whether haplotypes might be restricted to certain crops. No specialization was found; however, some haplotypes had a broader host range than others. These results suggest that the dispersal of H. armigera in the New World is occurring from both natural migration and human-mediated introductions. As such, both means of introduction should be monitored to prevent the spread of H. armigera into areas such as the United States, Mexico, and Canada, where it is not yet established.

Effect of diet delivered various concentrations of double-stranded RNA in silencing a midgut and a non-midgut gene of Helicoverpa armigera

2013 ◽  
Vol 103 (5) ◽  
pp. 555-563 ◽  
Author(s):  
R. Asokan ◽  
G. Sharath Chandra ◽  
M. Manamohan ◽  
N.K. Krishna Kumar
Keyword(s):  
Gene Silencing ◽  
Juvenile Hormone ◽  
Pest Management ◽  
Helicoverpa Armigera ◽  
Target Gene ◽  
Specific Gene ◽  
Upstream Gene ◽  
Double Stranded Rna ◽  
Helicoverpa Armígera ◽  
Rnai Response

AbstractRibonucleic acid interference (RNAi) is a sequence-specific gene silencing mechanism induced by double-stranded RNA (dsRNA). Recently, RNAi has gained popularity as a reverse genetics tool owing to its tremendous potential in insect pest management, which includes Helicoverpa armigera. However, its efficiency is mainly governed by dsRNA concentration, frequency of application, target gene, etc. Therefore, to obtain a robust RNAi response in H. armigera, we evaluated various concentrations of dsRNA and its frequency of applications delivered through diet in silencing a midgut gene, chymotrypsin and a non-midgut gene, juvenile hormone acid methyl transferase (jhamt) of H. armigera. The extent of target gene silencing was determined by employing reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Our study revealed four significant findings: (i) single application of dsRNA elicited a delayed and transient silencing, while multiple applications resulted in early and persistent silencing of the above genes; (ii) silencing of the non-midgut gene (jhamt) through diet delivered dsRNA revealed prevalence of systemic silencing probably due to communication of silencing signals in this pest; (iii) the extent of silencing of chymotrypsin was positively correlated with dsRNA concentration and was negatively correlated with jhamt; (iv) interestingly, over-expression (15–18 folds) of an upstream gene, farnesyl diphosphate synthase (fpps), in juvenile hormone (JH) biosynthetic pathway at higher concentrations of jhamt dsRNA was the plausible reason for lesser silencing of jhamt. This study provides an insight into RNAi response of target genes, which is essential for RNAi design and implementation as a pest management strategy.

scholarly journals Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113286 ◽  
Author(s):  
Natália A. Leite ◽  
Alessandro Alves-Pereira ◽  
Alberto S. Corrêa ◽  
Maria I. Zucchi ◽  
Celso Omoto
Keyword(s):  
Genetic Variability ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
New World ◽  
Old World ◽  
Helicoverpa Armígera ◽  
Old World Bollworm

scholarly journals Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses

2002 ◽  
Vol 83 (3) ◽  
pp. 673-684 ◽  
Author(s):  
Xinwen Chen ◽  
W.-J. Zhang ◽  
J. Wong ◽  
G. Chun ◽  
A. Lu ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Sequence Data ◽  
Biological Data ◽  
Open Reading Frames ◽  
Virus Species ◽  
Coding Regions ◽  
Helicoverpa Armígera ◽  
Small Orfs

The complete nucleotide sequence of Helicoverpa zea single-nucleocapsid nucleopolyhedrovirus (HzSNPV) has been determined (130869 bp) and compared to the nucleotide sequence of Helicoverpa armigera (Ha) SNPV. These two genomes are very similar in their nucleotide (97% identity) and amino acid (99% identity) sequences. The coding regions are much more conserved than the non-coding regions. In HzSNPV/HaSNPV, the 63 open reading frames (ORFs) present in all baculoviruses sequenced so far are much more conserved than other ORFs. HzSNPV has four additional small ORFs compared with HaSNPV, one of these (Hz42) being in a correct transcriptional context. The major differences between HzSNPV and HaSNPV are found in the sequence and organization of the homologous regions (hrs) and the baculovirus repeat ORFs (bro genes). The sequence identity between the HzSNPV and HaSNPV hrs ranges from 90% (hr1) to almost 100% (hr5) and the hrs differ in the presence/absence of one or more type A and/or B repeats. The three HzSNPV bro genes differ significantly from those in HaSNPV and may have been acquired independently in the ancestral past. The sequence data suggest strongly that HzSNPV and HaSNPV are variants of the same virus species, a conclusion that is supported by the physical and biological data.

scholarly journals Assay Optimization Can Equalize the Sensitivity of Real-Time PCR with ddPCR for Detection of Helicoverpa armigera (Lepidoptera: Noctuidae) in Bulk Samples

Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 885
Author(s):  
Thayssa M. R. Oliveira ◽  
Frida A. Zink ◽  
Renato C. Menezes ◽  
Érico C. Dianese ◽  
Karina C. Albernaz-Godinho ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Helicoverpa Armigera ◽  
Real Time Pcr ◽  
Helicoverpa Zea ◽  
Agricultural Pests ◽  
Assay Optimization ◽  
Extraction And Purification ◽  
Assay Performance ◽  
Helicoverpa Armígera

Helicoverpa armigera (Hübner) is one of the most important agricultural pests in the world. This historically Old World species was first reported in Brazil in 2013 and has since spread throughout much of South America and into the Caribbean. Throughout North America, H. armigera surveys are ongoing to detect any incursions. Each trap is capable of capturing hundreds of native Helicoverpa zea (Boddie). The two species cannot be separated without genitalic dissection or molecular methods. A ddPCR assay is currently used to screen large trap samples, but this equipment is relatively uncommon and expensive. Here, we optimized a newly designed assay for accurate and repeatable detection of H. armigera in bulk samples across both ddPCR and less costly, and more common, real-time PCR methods. Improvements over previously designed assays were sought through multiple means. Our results suggest bulk real-time PCR assays can be improved through changes in DNA extraction and purification, so that real-time PCR can be substituted for ddPCR in screening projects. While ddPCR remains a more sensitive method for detection of H. armigera in bulk samples, the improvements in assay design, DNA extraction, and purification presented here also enhance assay performance over previous protocols.

Sign in / Sign up

Export Citation Format

Share Document