Spatio‐temporal distribution of DMI and SDHI fungicide resistance of Zymoseptoria tritici throughout Europe based on frequencies of key target‐site alterations

2021 ◽  
Author(s):  
Pierre Hellin ◽  
Maxime Duvivier ◽  
M. Thies Heick ◽  
A. Fraaije Bart ◽  
Charlotte Bataille ◽  
...  
Keyword(s):  
Fungicide Resistance ◽  
Temporal Distribution ◽  
Target Site ◽  
Zymoseptoria Tritici ◽  
Spatio Temporal

scholarly journals Development of a PacBio Long-Read Sequencing Assay for High Throughput Detection of Fungicide Resistance in Zymoseptoria tritici

2021 ◽  
Vol 12 ◽  
Author(s):  
Berit Samils ◽  
Björn Andersson ◽  
Eva Edin ◽  
Malin Elfstrand ◽  
Tilman Rönneburg ◽  
...  
Keyword(s):  
High Throughput ◽  
Fungicide Resistance ◽  
Housekeeping Genes ◽  
Target Site ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Zymoseptoria Tritici ◽  
Multiplex Amplification ◽  
Long Read

Fungicide resistance has become a challenging problem in management of Septoria tritici blotch (STB), caused by Zymoseptoria tritici, the most destructive disease of winter wheat throughout western and northern Europe. To ensure the continued effectiveness of those fungicides currently used, it is essential to monitor the development and spread of such resistance in field populations of the pathogen. Since resistance to the key families of fungicides used for STB control (demethyalation inhibitors or azoles, succinate dehydrogenase inhibitors or SDHIs and Quinone outside Inhibitors or QoIs) is conferred through target-site mutations, the potential exists to monitor resistance through the molecular detection of alterations in the target site genes. As more efficient fungicides were developed and applied, the pathogen has continuously adapted through accumulating multiple target-site alterations. In order to accurately monitor these changes in field populations, it is therefore becoming increasingly important to completely sequence the targeted genes. Here we report the development of a PacBio assay that facilitates the multiplex amplification and long-read sequencing of the target gene(s) for the azole (CYP51), SDHI (Sdh B, C, and D), and QoI (cytochrome b) fungicides. The assay was developed and optimised using three Irish Z. tritici collections established in spring 2017, which capture the range of fungicide resistance present in modern European populations of Z. tritici. The sequences obtained through the PacBio assay were validated using traditional Sanger sequencing and in vitro sensitivity screenings. To further exploit the long-read and high throughput potential of PacBio sequencing, an additional nine housekeeping genes (act, BTUB, cal, cyp, EF1, GAPDH, hsp80-1, PKC, TFC1) were sequenced and used to provide comprehensive Z. tritici strain genotyping.

Influence of Planting Density on Root Spatio-Temporal Distribution of Different Types of Maize under High-Yielding Cultivation Conditions

2013 ◽  
Vol 38 (7) ◽  
pp. 1286-1294 ◽  
Author(s):  
Zong-Xin LI ◽  
Yuan-Quan CHEN ◽  
Qing-Cheng WANG ◽  
Kai-Chang LIU ◽  
Wang-Sheng GAO ◽  
...  
Keyword(s):  
Temporal Distribution ◽  
Planting Density ◽  
Cultivation Conditions ◽  
Different Types ◽  
Spatio Temporal

SPATIO-TEMPORAL DISTRIBUTION OF URANIUM CONCENTRATION IN SHALLOW AQUIFERS OF ARAVALLI TERRANE (NORTH GUJARAT), INDIA

2019 ◽  
Author(s):  
Rudra Mohan Pradhan ◽  
◽  
Karrie A. Weber ◽  
Karrie A. Weber ◽  
Daniel Snow ◽  
...  
Keyword(s):  
Uranium Concentration ◽  
Temporal Distribution ◽  
Shallow Aquifers ◽  
Spatio Temporal ◽  
North Gujarat

scholarly journals Variation Characteristics and Transportation of Aerosol, NO2, SO2, and HCHO in Coastal Cities of Eastern China: Dalian, Qingdao, and Shanghai

2021 ◽  
Vol 13 (5) ◽  
pp. 892
Author(s):  
Xiaomei Li ◽  
Pinhua Xie ◽  
Ang Li ◽  
Jin Xu ◽  
Zhaokun Hu ◽  
...  
Keyword(s):  
Eastern China ◽  
Temporal Distribution ◽  
Correlation Coefficients ◽  
Optical Absorption Spectroscopy ◽  
Near Surface ◽  
Mixing Ratios ◽  
Variation Characteristics ◽  
Spatio Temporal ◽  
Gas Volume ◽  
Transport Flux

This paper studied the method for converting the aerosol extinction to the mass concentration of particulate matter (PM) and obtained the spatio-temporal distribution and transportation of aerosol, nitrogen dioxide (NO2), sulfur dioxide (SO2), and formaldehyde (HCHO) based on multi-axis differential optical absorption spectroscopy (MAX-DOAS) observations in Dalian (38.85°N, 121.36°E), Qingdao (36.35°N, 120.69°E), and Shanghai (31.60°N, 121.80°E) from 2019 to 2020. The PM2.5 measured by the in situ instrument and the PM2.5 simulated by the conversion formula showed a good correlation. The correlation coefficients R were 0.93 (Dalian), 0.90 (Qingdao), and 0.88 (Shanghai). A regular seasonality of the three trace gases is found, but not for aerosols. Considerable amplitudes in the weekly cycles were determined for NO2 and aerosols, but not for SO2 and HCHO. The aerosol profiles were nearly Gaussian, and the shapes of the trace gas profiles were nearly exponential, except for SO2 in Shanghai and HCHO in Qingdao. PM2.5 presented the largest transport flux, followed by NO2 and SO2. The main transport flux was the output flux from inland to sea in spring and winter. The MAX-DOAS and the Copernicus Atmosphere Monitoring Service (CAMS) models’ results were compared. The overestimation of NO2 and SO2 by CAMS is due to its overestimation of near-surface gas volume mixing ratios.

scholarly journals Integrated structured light architectures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Randy Lemons ◽  
Wei Liu ◽  
Josef C. Frisch ◽  
Alan Fry ◽  
Joseph Robinson ◽  
...  
Keyword(s):  
Degrees Of Freedom ◽  
Temporal Distribution ◽  
Multiple Degrees Of Freedom ◽  
Beam Combination ◽  
Angular Momenta ◽  
Field Control ◽  
Topological States ◽  
Spatio Temporal ◽  
Temporal Field ◽  
Structural Versatility

AbstractThe structural versatility of light underpins an outstanding collection of optical phenomena where both geometrical and topological states of light can dictate how matter will respond or display. Light possesses multiple degrees of freedom such as amplitude, and linear, spin angular, and orbital angular momenta, but the ability to adaptively engineer the spatio-temporal distribution of all these characteristics is primarily curtailed by technologies used to impose any desired structure to light. We demonstrate a laser architecture based on coherent beam combination offering integrated spatio-temporal field control and programmability, thereby presenting unique opportunities for generating light by design to exploit its topology.

Sign in / Sign up

Export Citation Format

Share Document