Phenotypic and molecular analyses in rice weevil, Sitophilus oryzae ( L inneaus) ( C oleoptera: C urculionidae): identification of a super kdr mutation, T929I, conferring resistance to deltamethrin

2021 ◽  
Author(s):  
Sonu K Singh ◽  
Rajeswaran Jagadeesan ◽  
Sonai Rajan Thangaraj ◽  
Upasna Selvapandian ◽  
Manoj K Nayak ◽  
...  
Keyword(s):  
Sitophilus Oryzae ◽  
Rice Weevil ◽  
Kdr Mutation ◽  
Molecular Analyses

scholarly journals Potential insecticidal activity of four essential oils against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7767-7783
Author(s):  
Mohamed E. Tawfeek ◽  
Hayssam M. Ali ◽  
Mohammad Akrami ◽  
Mohamed Z. M. Salem
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Sitophilus Oryzae ◽  
Imperata Cylindrica ◽  
Rice Weevil ◽  
Gas Chromatograph ◽  
Cymbopogon Citratus ◽  
Phytochemical Composition ◽  
Germacrene D ◽  
Gas Chromatograph Mass Spectrometry

Oils extracted from Cymbopogon citratus, Lantana camara, Artemisia camphorata, and Imperata cylindrica plants were used as potential insecticides against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae). The phytochemical composition of the isolated oils was identified by gas chromatograph-mass spectrometry (GC-MS). Oil contact toxicities were evaluated against the adults of S. oryzae. The activities of acetylcholinesterase (AChE), alkaline phosphatase (ALP), and transaminases enzymes (AST) were measured. L. camara oil (LC50 = 9.81 mg/cm2) demonstrated the highest effect, followed by C. citratus oil (LC50 = 10.89 mg/cm2), A. camphorata EO (LC50 = 16.12 mg/cm2), and I. cylindrica oil (LC50= 36.85 mg/cm2) against the adults of S. oryzae. The inhibition percentages of AChE were 38.8, 41.7, 35.0, and 27.2%; ALP were 42.4, 49.3, 28.1, and 18.7%; AST were 33.9, 38.7, 20.8, and 11.8%; and ALT were 22.7, 30.5, 14.6, and 9.6% after treated S. oryzae with oils from C. citratus, L. camara, A. camphorata and I. cylindrica, respectively. The highest abundant compounds in C. citratus were geranial (25.95%), nerylacetal (8.85%), and neral (8.45%), in L. camara were caryophyllene (12.2%), and 3-elemene (8.89%), in A. camphorata were germacrene D-4-ol (20.83%), and borneol (19.47%), and in I. cylindrica were 5-phenylundecane (10.68%), and 6-phenyldodecane (8.70%).

scholarly journals Minimization of energy transduction confers resistance to phosphine in the rice weevil, Sitophilus oryzae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kyeongnam Kim ◽  
Jeong Oh Yang ◽  
Jae-Yoon Sung ◽  
Ji-Young Lee ◽  
Jeong Sun Park ◽  
...  
Keyword(s):  
Cancer Metabolism ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Resistance Mechanism ◽  
Energy Transduction ◽  
Metabolic Reprogramming ◽  
Rice Weevil ◽  
Altered Expression ◽  
Synonymous Mutations ◽  
Genes Encoding

Abstract Infestation of phosphine (PH3) resistant insects threatens global grain reserves. PH3 fumigation controls rice weevil (Sitophilus oryzae) but not highly resistant insect pests. Here, we investigated naturally occurring strains of S. oryzae that were moderately resistant (MR), strongly resistant (SR), or susceptible (wild-type; WT) to PH3 using global proteome analysis and mitochondrial DNA sequencing. Both PH3 resistant (PH3–R) strains exhibited higher susceptibility to ethyl formate-mediated inhibition of cytochrome c oxidase than the WT strain, whereas the disinfectant PH3 concentration time of the SR strain was much longer than that of the MR strain. Unlike the MR strain, which showed altered expression levels of genes encoding metabolic enzymes involved in catabolic pathways that minimize metabolic burden, the SR strain showed changes in the mitochondrial respiratory chain. Our results suggest that the acquisition of strong PH3 resistance necessitates the avoidance of oxidative phosphorylation through the accumulation of a few non-synonymous mutations in mitochondrial genes encoding complex I subunits as well as nuclear genes encoding dihydrolipoamide dehydrogenase, concomitant with metabolic reprogramming, a recognized hallmark of cancer metabolism. Taken together, our data suggest that reprogrammed metabolism represents a survival strategy of SR insect pests for the compensation of minimized energy transduction under anoxic conditions. Therefore, understanding the resistance mechanism of PH3–R strains will support the development of new strategies to control insect pests.

scholarly journals Susceptibility of six varieties of rice to the infestation of rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

2012 ◽  
Vol 21 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Humayun Reza Khan ◽  
Polash Kanti Halder
Keyword(s):  
Oryza Sativa ◽  
Emerging Adults ◽  
Sitophilus Oryzae ◽  
Ambient Condition ◽  
Rice Weevil ◽  
Rice Varieties ◽  
Moisture Contents ◽  
The Mean

The susceptibility of six varieties of rice, Oryza sativa, viz. Lata, Minicate, Nazersail, Parija, Kalijira and Kataribhog, to the infestation of the rice weevil, Sitophilus oryzae (L.), was studied on the basis of population build up under an ambient condition (28 ± 4ºC and 70 ± 4 % RH) of the laboratory. The mean weights of each grain of the above six rice varieties were 16.9, 16.1, 11.43, 13.87, 6.23 and 9.77 mg, respectively; mean lengths were 6.47, 6.56, 5.37, 5.38, 4.22 and 4.92 mm, respectively; mean widths were 2.45, 2.03, 2.05, 2.17, 1.6 and 1.9 mm, respectively; and moisture contents were 11.55, 10.75, 11.6, 12.71, 11.85, and 12.1 per cents, respectively. As far as the number of emerging adults is concerned, the weevil showed the highest number (695) in Nazersail on the 16th week, Lata (755) on the 18th week, Minicate (654) on the 16th week, Parija (482) on the 20th week, Kalijira (402) on the 20th week, and Kataribhog (456) on the 20th week. The mean numbers of the adult weevils in the above six varieties after 22 weeks of rearing were 425, 410, 351, 387, 357 and 400, respectively and was statistically significant at 5% level. The degree of susceptibility of the rice varieties to the rice weevils from the highest to lowest susceptibility was ? Lata > Nazersail > Minicate > Pariza > Kataribhog > Kalijira.DOI: http://dx.doi.org/10.3329/dujbs.v21i2.11514 Dhaka Univ. J. Biol. Sci. 21(2): 163-168, 2012 (July)

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