scholarly journals Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

2020 ◽  
Author(s):  
Debora Boaventura ◽  
Benjamin Buer ◽  
Niklas Hamaekers ◽  
Frank Maiwald ◽  
Ralf Nauen
Keyword(s):  
Insecticide Resistance ◽  
Fall Armyworm ◽  
Molecular Profiling ◽  
Brazilian Strain

scholarly journals Insecticide resistance monitoring for the invasive populations of fall armyworm, Spodoptera frugiperda in China

2021 ◽  
Vol 20 (3) ◽  
pp. 783-791 ◽  
Author(s):  
Dan-dan ZHANG ◽  
Yu-tao XIAO ◽  
Peng-jun XU ◽  
Xian-ming YANG ◽  
Qiu-lin WU ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Resistance Monitoring

scholarly journals Adaptation by copy number variation increases insecticide resistance in fall armyworms

2019 ◽  
Author(s):  
Kiwoong Nam ◽  
Sylvie Gimenez ◽  
Frederique Hilliou ◽  
Carlos A. Blanco ◽  
Sabine Hänniger ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
Insecticide Resistance ◽  
Host Plant ◽  
Copy Number ◽  
Host Plants ◽  
Fall Armyworm ◽  
Copy Number Variations ◽  
Main Challenge ◽  
Number Variation ◽  
Allelic Differentiation

AbstractInsecticide resistance is a major main challenge in pest control, and understanding its genetic basis is a key topic in agricultural ecology. Detoxification genes are well-known genetic elements that play a key role in adaptation to xenobiotics. The adaptive evolution of detoxification genes by copy number variations has been interpreted as a cause of insecticide resistance. However, the same pattern can be generated by the adaptation to host-plant defense toxins as well. In this study, we tested in fall armyworms (Lepidoptera Spodoptera frugiperda) if adaptation by copy number variation is the cause of the increased level of insecticide resistance from two geographic populations with different levels of resistance and two strains with different host plants. Following the generation of an assembly with chromosome-sized scaffolds (N50 = 13.2Mb), we observed that these two populations show a significant allelic differentiation of copy number variations, which is not observed between strains. In particular, a locus with almost complete allelic differentiation (Fst > 0.8) includes a cluster of P450 genes, which are well-known key players in detoxification. Detoxification genes are overrepresented in the genes with copy number variations, and the observed copy number variation appears to have beneficial effects in general. From this result, we concluded that copy number variation of detoxification genes in fall armyworms plays a key role in the insecticide resistance but not in the adaptation to host-plants, suggesting that the evolution of insecticide resistance may occur independently from host-plant adaptation.

scholarly journals Geographic Monitoring of Insecticide Resistance Mutations in Native and Invasive Populations of the Fall Armyworm

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 468
Author(s):  
Sudeeptha Yainna ◽  
Nicolas Nègre ◽  
Pierre J. Silvie ◽  
Thierry Brévault ◽  
Wee Tek Tay ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Fall Armyworm ◽  
Sub Saharan Africa ◽  
Pest Species ◽  
Resistance Mutations ◽  
Chinese Populations ◽  
Bt Resistance ◽  
Native Populations ◽  
Sub Saharan ◽  
Lepidopteran Pest

Field evolved resistance to insecticides is one of the main challenges in pest control. The fall armyworm (FAW) is a lepidopteran pest species causing severe crop losses, especially corn. While native to the Americas, the presence of FAW was confirmed in West Africa in 2016. Since then, the FAW has been detected in over 70 countries covering sub-Saharan Africa, the Middle East, North Africa, South Asia, Southeast Asia, and Oceania. In this study, we tested whether this invasion was accompanied by the spread of resistance mutations from native to invasive areas. We observed that mutations causing Bt resistance at ABCC2 genes were observed only in native populations where the mutations were initially reported. Invasive populations were found to have higher gene numbers of cytochrome P450 genes than native populations and a higher proportion of multiple resistance mutations at acetylcholinesterase genes, supporting strong selective pressure for resistance against synthetic insecticides. This result explains the susceptibility to Bt insecticides and resistance to various synthetic insecticides in Chinese populations. These results highlight the necessity of regular and standardized monitoring of insecticide resistance in invasive populations using both genomic approaches and bioassay experiments.

Monitoring and molecular profiling of contemporary insecticide resistance status of malaria vectors in Guinea–Bissau

Acta Tropica ◽  
2020 ◽  
Vol 206 ◽  
pp. 105440
Author(s):  
Ronise Silva ◽  
Konstantinos Mavridis ◽  
John Vontas ◽  
Amabélia Rodrigues ◽  
Hugo Costa Osório
Keyword(s):  
Insecticide Resistance ◽  
Molecular Profiling ◽  
Malaria Vectors ◽  
Guinea Bissau

Rapid selection and characterization of Cry1F resistance in a Brazilian strain of fall armyworm

2016 ◽  
Vol 158 (3) ◽  
pp. 236-247 ◽  
Author(s):  
Natália A. Leite ◽  
Simone M. Mendes ◽  
Oscar F. Santos-Amaya ◽  
Chritiane A. Santos ◽  
Thaís P. M. Teixeira ◽  
...  
Keyword(s):  
Fall Armyworm ◽  
Brazilian Strain

scholarly journals Monitoring of Target-Site Mutations Conferring Insecticide Resistance in Spodoptera frugiperda

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 545 ◽  
Author(s):  
Debora Boaventura ◽  
Macarena Martin ◽  
Alberto Pozzebon ◽  
David Mota-Sanchez ◽  
Ralf Nauen
Keyword(s):  
Insecticide Resistance ◽  
Spodoptera Frugiperda ◽  
Resistance Management ◽  
Fall Armyworm ◽  
Target Site ◽  
Diagnostic Methods ◽  
Allelic Discrimination ◽  
Tropical Regions ◽  
Voltage Gated Sodium Channels ◽  
Major Pest

Fall armyworm (FAW), Spodoptera frugiperda, a major pest of corn and native to the Americas, recently invaded (sub)tropical regions worldwide. The intensive use of insecticides and the high adoption of crops expressing Bacillus thuringiensis (Bt) proteins has led to many cases of resistance. Target-site mutations are among the main mechanisms of resistance and monitoring their frequency is of great value for insecticide resistance management. Pyrosequencing and PCR-based allelic discrimination assays were developed and used to genotype target-site resistance alleles in 34 FAW populations from different continents. The diagnostic methods revealed a high frequency of mutations in acetylcholinesterase, conferring resistance to organophosphates and carbamates. In voltage-gated sodium channels targeted by pyrethroids, only one population from Indonesia showed a mutation. No mutations were detected in the ryanodine receptor, suggesting susceptibility to diamides. Indels in the ATP-binding cassette transporter C2 associated with Bt-resistance were observed in samples collected in Puerto Rico and Brazil. Additionally, we analyzed all samples for the presence of markers associated with two sympatric FAW host plant strains. The molecular methods established show robust results in FAW samples collected across a broad geographical range and can be used to support decisions for sustainable FAW control and applied resistance management.

Sign in / Sign up

Export Citation Format

Share Document