Rapid selection and characterization of Cry1F resistance in a Brazilian strain of fall armyworm

2016 ◽  
Vol 158 (3) ◽  
pp. 236-247 ◽  
Author(s):  
Natália A. Leite ◽  
Simone M. Mendes ◽  
Oscar F. Santos-Amaya ◽  
Chritiane A. Santos ◽  
Thaís P. M. Teixeira ◽  
...  
Keyword(s):  
Fall Armyworm ◽  
Brazilian Strain

scholarly journals Isolation and DNA Barcode Characterization of a PermanentTelenomus(Hymenoptera: Platygastridae) Population in Florida That Targets Fall Armyworm (Lepidoptera: Noctuidae)

2015 ◽  
Vol 108 (5) ◽  
pp. 729-735 ◽  
Author(s):  
Mirian M. Hay-Roe ◽  
Rodney N. Nagoshi ◽  
Robert L. Meagher ◽  
Myriam Arias De Lopez ◽  
Rogelio Trabanino
Keyword(s):  
Dna Barcode ◽  
Fall Armyworm ◽  
Lepidoptera Noctuidae

Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Author(s):  
Ingrid Zanella-Saenz ◽  
Elisabeth A. Herniou ◽  
Jorge E. Ibarra ◽  
Ma.Cristina Del Rincón-Castro ◽  
Ilse Alejandra Huerta-Arredondo
Keyword(s):  
Biological Control ◽  
Cell Cultures ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
The United States ◽  
Infection Mechanisms ◽  
Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

scholarly journals Genetic characterization of fall armyworm infesting South Africa and India indicate recent introduction from a common source population

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0217755 ◽  
Author(s):  
Rodney N. Nagoshi ◽  
Isabel Dhanani ◽  
R. Asokan ◽  
H. M. Mahadevaswamy ◽  
Chicknayakanahalli M. Kalleshwaraswamy ◽  
...  
Keyword(s):  
South Africa ◽  
Genetic Characterization ◽  
Fall Armyworm ◽  
Common Source ◽  
Source Population ◽  
Recent Introduction

scholarly journals The genetic characterization of fall armyworm populations in Ecuador and its implications to migration and pest management in the northern regions of South America

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0236759
Author(s):  
Rodney N. Nagoshi ◽  
Ernesto Cañarte ◽  
Bernardo Navarrete ◽  
Jimmy Pico ◽  
Catalina Bravo ◽  
...  
Keyword(s):  
South America ◽  
Pest Management ◽  
Genetic Characterization ◽  
Fall Armyworm ◽  
Northern Regions

scholarly journals Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm

2008 ◽  
Vol 43 (4) ◽  
pp. 513-520 ◽  
Author(s):  
Andréa Almeida Carneiro ◽  
Eliane Aparecida Gomes ◽  
Claudia Teixeira Guimarães ◽  
Fernando Tavares Fernandes ◽  
Newton Portilho Carneiro ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spodoptera Frugiperda ◽  
Sequence Comparison ◽  
Rapd Markers ◽  
Fall Armyworm ◽  
Its Sequence ◽  
Its Sequencing ◽  
Host Insect ◽  
Rdna Its

The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.

Characterization of a novel peptide with allatotropic activity in the fall armyworm Spodoptera frugiperda

2004 ◽  
Vol 122 (2) ◽  
pp. 69-78 ◽  
Author(s):  
Mohatmed Abdel-latief ◽  
Martina Meyering-Vos ◽  
Klaus H. Hoffmann
Keyword(s):  
Spodoptera Frugiperda ◽  
Fall Armyworm

scholarly journals Molecular and Biological Characterization of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Field Isolate and Genotypes from China

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 777
Author(s):  
Chengfeng Lei ◽  
Jian Yang ◽  
Jia Wang ◽  
Jia Hu ◽  
Xiulian Sun
Keyword(s):  
Spodoptera Frugiperda ◽  
Field Isolate ◽  
Management Strategies ◽  
Fall Armyworm ◽  
Multiple Nucleopolyhedrovirus ◽  
Cloning Method ◽  
Occlusion Bodies ◽  
Colombian Isolate

The fall armyworm, Spodoptera frugiperda, is a new invading pest in China. The baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a pathogenic agent of the fall armyworm and a potential agent for its control in integrated pest management strategies. In this work, we analyze the molecular and biological characteristics of an SfMNPV isolate collected from maize in China (SfMNPV-Hub). Two genotypes were further isolated from SfMNPV-Hub by an in vivo cloning method. The PstI profile of one genotype (SfHub-A) was similar to genotype A of the SfMNPV Colombian isolate, and the other (SfHub-E) was similar to genotype E of the Colombian isolate. The bioactivity of SfHub-A against second-instar S. frugiperda larvae was not significantly different from that of SfMNPV-Hub, whereas SfHub-E was 2.7–5.5 fold less potent than SfMNPV-Hub. The speed of kill of SfHub-E was quicker than SfMNPV-Hub, while SfHub-A acted slower than SfMNPV-Hub. Occlusion body (OB) production of SfHub-A in an S. frugiperda cadaver was significantly higher than that of SfMNPV-Hub, while SfHub-E yielded far fewer occlusion bodies (OBs) in the host larvae. These results provide basic information for developing a virus-based pesticide against the invading pest S. frugiperda.

scholarly journals Molecular characterization of the Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on maize in southern Rajasthan, India

2021 ◽  
Author(s):  
Sakthivel Ramesh Babu ◽  
Perumal Pachippan ◽  
Raja Manoharan ◽  
Sonika Joshi ◽  
Deepika Kalyan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Caribbean Region ◽  
Plant Identification ◽  
African Region ◽  
Tropical America ◽  
Lepidopteran Pest ◽  
Lepidoptera Noctuidae

Abstract The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is a polyphagous Lepidopteran pest, a native to tropical and sub-tropical America and recently it has invaded the African and Asian countries. Presently, the mitochondrial Cytochrome Oxidase Subunit I (COI) - gene based molecular characterization of FAW samples from the maize fields of southern Rajasthan has revealed the occurrence of corn and rice strains there. The occurrence of such S. frugiperda population of Rajasthan region could be traced its origin from the Florida-Caribbean region or African region. Further, the Tpi gene region analysis showed that the S.frugiperda forms found in the maize fields are only the corn strains. In the Indian Rajasthan populations of FAW, the Tpi-variant2 category is the highest one and is then followed by the Tpi-variant1 and Tpi-variant3 was unique with C and T at Tpie4192 and Tpie4198, respectively. Further research is needed towards the confirmation of these tentatively identified strains of S. frugiperda that would in turn helpful for the proper monitoring, host-plant identification and the effective management of such pests.

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