Antifungal activity of rapamycin on Botryosphaeria dothidea and its effect against Chinese hickory canker

2020 ◽  
Vol 77 (1) ◽  
pp. 425-431
Author(s):  
Hong Jiang ◽  
Yuxin Rao ◽  
Li Mei ◽  
Yongjun Wang
Keyword(s):  
Antifungal Activity ◽  
Botryosphaeria Dothidea

scholarly journals Potential of Trichoderma spp. and Pinus sylvestris Bark Extracts as Biocontrol Agents against Fungal Pathogens Residing in the Botryosphaeriales

2020 ◽  
Vol 3 (1) ◽  
pp. 99
Author(s):  
Vera Karličić ◽  
Jelena Jovičić-Petrović ◽  
Veljko Marojević ◽  
Milica Zlatković ◽  
Saša Orlović ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Pinus Sylvestris ◽  
Growth Inhibition ◽  
Urban Areas ◽  
Fungal Pathogens ◽  
Water Solution ◽  
Biocontrol Agents ◽  
Botryosphaeria Dothidea ◽  
Trichoderma Spp ◽  
Trichoderma Isolates

Botryosphaeriales represent a diverse order of fungal pathogens of various woody plant species. In Serbia, these fungi are important pathogens of forest, ornamental, and fruit trees causing die-back, cankers, leaf blights, fruit, and root rot. The aim of this study was to evaluate the antifungal activity of Pinus sylvestris bark extracts and Trichoderma spp. against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Ascomycota, Botryosphaeriales) isolated from Picea abies, Thuja occidentalis, and Prunus laurocerasus trees planted in urban areas in Serbia. Bark extracts were prepared in water solution at two temperatures (80 and 120 °C). The extracts were tested using two concentrations (20% and 30%). Moreover, two Trichoderma isolates obtained from P. sylvestris bark were tested against Botryosphaeriales and their antagonistic potential was estimated in vitro using a confrontation test. Mycelial growth of B. dothidea and D. sarmentorum was significantly inhibited in the presence of bark extracts, while N. parvum showed no growth inhibition. Botryosphaeria dothidea growth was inhibited by 35 to 39% in the case of 20% extracts and by 39 to 44% in the case of 30% extracts. The growth inhibition of D. sarmentorum was between 48% and 56% in the case of 20% extracts and between 53% and 60% in the case of 30% extracts. The two Trichoderma isolates showed antifungal activity against the selected pathogens. An isolate BKG 4 showed the highest inhibition level, and it inhibited the growth of B. dothidea, D. sarmentorum, and N. parvum by 85%, 75%, and 62%, respectively. Preliminary results suggest that both P. sylvestris bark extracts and Trichoderma spp. could be used as biocontrol agents against B. dothidea, D. sarmentorum, and N. parvum, and this should be a further studied.

scholarly journals Synthesis and Antifungal Activity of Pyrimidine Derivatives Containing an Amide Moiety

2021 ◽  
Vol 9 ◽  
Author(s):  
Wenneng Wu ◽  
Wenjun Lan ◽  
Chenyan Wu ◽  
Qiang Fei
Keyword(s):  
Antifungal Activity ◽  
Biological Test ◽  
Inhibition Rate ◽  
Botryosphaeria Dothidea ◽  
Pyrimidine Derivatives ◽  
Antifungal Activities ◽  
First Report ◽  
Better Than

In this study, 17 novel pyrimidine derivatives containing an amide moiety were synthesized. Then their in vitro antifungal activities against Botryosphaeria dothidea (B. dothidea), Phomopsis sp., and Botrytis cinereal (B. cinereal) were determined. A preliminary biological test showed that compounds 5-bromo-2-fluoro-N-(2-((2-methyl-6-(trifluoromethyl)pyrimidin-4-yl)oxy)phenyl)benzamide (5f) and 5-bromo-2-fluoro-N-(3-((2-methyl-6-(trifluoromethyl)pyrimidin-4-yl)oxy)phenyl)benzamide (5o) exhibited higher antifungal activity against Phomopsis sp., with an inhibition rate of 100% compared to that of Pyrimethanil at 85.1%. In particular, compound 5o exhibited excellent antifungal activity against Phompsis sp., with the EC50 value of 10.5 μg/ml, which was even better than that of Pyrimethanil (32.1 μg/ml). As far as we know, this is the first report on the antifungal activities against B. dothidea, Phomopsis sp., and B. cinereal of this series of pyrimidine derivatives containing an amide moiety.

scholarly journals Inhibition Mechanism of Caffeine in Tea Pathogenic Fungi Botryosphaeria Dothidea and Colletotrichum Gloeosporioides

2020 ◽  
Author(s):  
Kuberan Thangaraj ◽  
Cheng Deng ◽  
Lin-Lin Cheng ◽  
Wei-Wei Deng ◽  
Zheng-Zhu Zhang
Keyword(s):  
Gene Expression ◽  
Antifungal Activity ◽  
Ribosome Biogenesis ◽  
High Throughput Sequencing ◽  
Colletotrichum Gloeosporioides ◽  
Host Cells ◽  
Inhibition Mechanism ◽  
Botryosphaeria Dothidea ◽  
Tea Leaves ◽  
Melanin Biosynthesis

Abstract Background Caffeine acts as an antifungal agent; however, its underlying inhibition mechanism remains unclear. To investigate variations in caffeine antifungal activity, this study was evaluate the inhibition mechanisms of caffeine on phytopathogens Botryosphaeria dothidea and Colletotrichum gloeosporioides through transcriptomic analysis, which causes leaf necrosis and anthracnose on tea leaves, respectively.Results Our in vitro results shows that the antifungal activity of caffeine quite different between B. dothidea and C. gloeosporioides. Furthermore, microscopic observation and bioactivity assay results proved that caffeine destroyed the fungal protective layers (cell wall and cell membrane) and subcellular organelles. The transcriptomes of these two fungi exposed to different caffeine concentrations were analyzed by high-throughput sequencing and the results showed that caffeine repressed gene expression involved in the pathways of mRNA surveillance, ribosome biogenesis in eukaryotes, aminoacyl-tRNA biosynthesis, etc. Particularly, caffeine affected gene expression in melanin biosynthesis in C. gloeosporioides, resulting in the disturbances of melanin production and fungal pathogenicity. Manually infected tea leaves showed melanin pigment was important to appressorium development and fungal infection by histopathological observation and fungal biomass quantification. Besides, C. gloeosporioides successfully enters into the host tissue even plant could build multilayer defenses against the fungus, this might be due to the variation in pathogen targets and quantities of secondary metabolites by the host cells.Conclusions Overall, our findings indicate that caffeine in the environment can reduce the fungal growth and physiological activities. However, the genome-wide expression showed different transcriptional profiles between these fungal isolates. Thus, we believe that target of the caffeine varied among isolates and melanin may play a major role in fungus caffeine tolerance. Further in-depth research required to conclude this hypothesis.

scholarly journals Antifungal Activity of Monoterpenes against Botryosphaeria dothidea

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Zhilin Zhang ◽  
Yongjian Xie ◽  
Xian Hu ◽  
Hongan Shi ◽  
Mi Wei ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Synergistic Effect ◽  
Antagonistic Effect ◽  
Botryosphaeria Dothidea ◽  
Negative Effects ◽  
Antifungal Effect ◽  
Natural Plant ◽  
Synthetic Chemicals ◽  
Natural Fungicide ◽  
Future Test

The development of natural plant extracts and essential oils will assist to decrease the negative effects of synthetic chemicals. The antifungal activity of 20 pure monoterpenes was assessed their efficacy against Botryosphaeria dothidea. 20 compounds were investigated the antifungal activity against B. dothidea at the concentration of 400 μg·mL−1. And components were used to the future test, which of the antifungal rate is greater than 50% against B. dothidea. Results from antifungal tests revealed that cuminaldehyde, β-citronellol, nerol, geraniol, citral and α-terpineol exhibited strong antifungal effect against B. dothidea. In addition, the antifungal activity of cuminaldehyde, β-citronellol, and geraniol had a highest effect toward B. dothidea with LC50 values of 105.15, 135.73, 132.69 μg·mL−1, respectively. In addition to the antagonistic effect of geraniol/β-citronellol (1/2) mixture, the combination of other compounds has synergistic effect on B. dothidea. The present results indicated that cuminaldehyde, geraniol, and β-citronellol are promising antifungal effect against B. dothidea and could be useful in the search for new natural fungicide. Several high activity monoterpenes and some combined with two monoterpenes were identified, and provided a rationale for pursuing further research on the fungicide and enhanced efficacy by the combined of fungicide.

scholarly journals Synthesis of Novel Thiazolyl Hydrazine Derivatives and Their Antifungal Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jianjun Zhu ◽  
Yazhen Chen ◽  
Fen Su ◽  
Peiyi Wang
Keyword(s):  
Antifungal Activity ◽  
Mycelial Growth ◽  
Antifungal Agents ◽  
Biological Assessment ◽  
Botryosphaeria Dothidea ◽  
Hydrazine Derivatives ◽  
Mycelial Growth Rate ◽  
Rate Method ◽  
In Vitro Antifungal Activity

A series of novel thiazolyl hydrazine derivatives 3a–3o were synthesized and evaluated for their in vitro antifungal activity against six phytopathogenic strains, namely, Botryosphaeria dothidea (B. d.), Gibberella sanbinetti (G. s.), Fusarium oxysporum (F. o.), Thanatephorus cucumeris (T. c.), Sclerotinia sclerotiorum (S. s.), and Verticillium dahliae (V. d.), by the classical mycelial growth rate method. Biological assessment results showed that most of these target compounds showed good antifungal activity toward tested strains. Especially, compound 3l showed excellent antifungal activities against B. d. and G. s. with relatively lower EC50 values of 0.59 and 0.69 µg/mL, respectively, which were extremely superior to those of commercial fungicides fluopyram, boscalid, and hymexazol and were comparable to those of carbendazim. Given the excellent bioactivity of designed compounds, this kind of thiazolyl hydrazine framework can provide a suitable point for exploring highly efficient antifungal agents.

Antifungal activity of a flavonoid-rich fraction from Costus spicatus leaves against dermatophytes

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
DN Silva ◽  
MJ Gonçalves ◽  
MT Amaral ◽  
MT Batista
Keyword(s):  
Antifungal Activity

Antifungal Activity of Brassica rapa-Derived Extracts Against F. oxysporum

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
JE Fierro ◽  
P Jiménez ◽  
ED Coy-Barrera
Keyword(s):  
Antifungal Activity ◽  
Brassica Rapa
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