A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae , is associated with resistance to pyrethroid insecticides

2013 ◽  
Vol 70 (8) ◽  
pp. 1249-1253 ◽  
Author(s):  
Stephen P Foster ◽  
Verity L Paul ◽  
Russell Slater ◽  
Anne Warren ◽  
Ian Denholm ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Sitobion Avenae ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Grain Aphid

scholarly journals High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations

2019 ◽  
Author(s):  
Kentaro Itokawa ◽  
Koji Yatsu ◽  
Tsuyoshi Sekizuka ◽  
Yoshihide Maekawa ◽  
Osamu Komagata ◽  
...  
Keyword(s):  
Amino Acid ◽  
Insecticide Resistance ◽  
Sodium Channel ◽  
Genomic Dna ◽  
Genomic Structure ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Probe Set ◽  
Capture Sequencing

AbstractInsects’ voltage-gated sodium channel (VGSC) is the primary target site of pyrethroid insecticides. Various amino acid substitutions in the VGSC protein are known to confer insecticide resistance and are selected under insecticide pressure. In the genome, the VGSC gene consists of more than 30 exons sparsely distributed across a large genomic region, which often exceeds 100 kbp. Due to this complex genomic structure of gene VGSC, it is usually challenging to genotype full coding nucleotide sequences (CDSs) of VGSC from individual genomic DNA (gDNA). In this study, we designed biotinylated oligonucleotide probes via annotated CDSs of VGSC of Asian tiger mosquito, Aedes albopictus. The probe set effectively concentrated (>80,000-fold) all targeted regions of gene VGSC from pooled barcoded Illumina libraries each constructed from individual A. albopictus gDNAs. The probe set also captured all homologous VGSC CDSs except tiny exons from the gDNA of other Culicinae mosquitos, A. aegypti and Culex pipiens complex, with comparable efficiency by virtue of the high conservation of VGSC at the nucleotide level. Furthermore, we developed an automated bioinformatic analysis pipeline to genotype VGSC after capture sequencing—MoNaS (Mosquito Na+ channel mutation Search)—which conducts mapping of reads, variant calling, and variant annotation for nonsynonymous mutations. The proposed method and our bioinformatic tool should facilitate the discovery of novel amino acid variants conferring insecticide resistance on VGSC and population genetics studies on resistance alleles with respect to the origin, selection, and migration of both clinically and agriculturally important insect pests.

scholarly journals Molecular Insight into the Knockdown Resistance (kdr) in the Voltage Gated Sodium Channel (vgsc) Gene of the Main Dengue Vector, Aedes aegypti (Diptera: Culicidae) and the Discovery of Novel Regional Specific Point Mutation A1007G in Malaysia.

2021 ◽  
Author(s):  
Mas Azlin M. Akhir ◽  
Mustafa F. F. Wajidi ◽  
Sébastien Lavoué ◽  
Ghows Azzam ◽  
Izhan Shahrin Jaafar ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Sodium Channel ◽  
Vector Control ◽  
Resistance Mechanism ◽  
Point Mutations ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Control Programmes ◽  
Pirimiphos Methyl

Abstract Background: Characterization of the insecticide resistance mechanism imparts the society with the information on the evolutionary process involved in the adaptation of Aedes aegypti mosquito to environmental changes. Investigating the phenotypic status of the target mosquitoes, their resistance level as well as elucidating the genotypic profile provides information about the involvement of insecticide resistance mechanism, in terms of portraying the evolution of resistance in the field, to eventually managing vector control programmes. In this current study, we investigated the quantification responses for the phenotypic and genotypic resistance of Ae. aegypti population from different states in Malaysia. Methods: We tested insecticide susceptibility status of adult Ae. aegypti from populations of States of Penang, Selangor and Kelantan (Peninsular Malaysia) against 0.25% permethrin and 0.25% pirimiphos-methyl through WHO bioassay kit. Permethrin-resistant and permethrin susceptible samples were then genotyped for domains II and III in the voltage gated sodium channel (vgsc) gene using allele specific PCR (AS-PCR) for the presence of diagnostic single nucleotide mutations. AS-PCR results were then validated in sequencing these two domains to identify any possible additional point mutations. Results: Adult WHO bioassay revealed that populations of Ae. aegypti from these three states were highly resistant towards 0.25% permethrin and 0.25% pirimiphos-methyl. Genotyping results showed that three knockdown (kdr) mutations (i.e. S989P, V1016G and F1534C) were associated with pyrethroid resistance in these populations. We also report for the first time the presence of the A1007G mutation in Malaysian populations of Ae. aegypti.Conclusions: This study brings an insight on the occurrence and association of point mutations with insecticide resistance in Malaysian populations of Ae. aegypti. The results reveal the widespread of several kdr mutations in the field with the consequence to compromise the use of pyrethroid insecticides in vector control programmes. Knowledge on the distribution of target site resistance throughout Malaysia is vital to ensure the success of the insecticide-based vector control programme.

scholarly journals First evidence of retained sexual capacity and survival in the pyrethroid resistant Sitobion avenae (F.) (Hemiptera: Aphididae) SA3 super-clone following exposure to a pyrethroid at current field-rate

2019 ◽  
Vol 58 (1) ◽  
pp. 21-26 ◽  
Author(s):  
L.E. Walsh ◽  
M.T. Gaffney ◽  
G.L. Malloch ◽  
S.P. Foster ◽  
M.S. Williamson ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Pest Management ◽  
Management Strategy ◽  
Resistance Management ◽  
Sitobion Avenae ◽  
Cereal Crops ◽  
Current Field ◽  
Pyrethroid Insecticides ◽  
Channel Gene ◽  
Grain Aphid

AbstractThe grain aphidSitobion avenaeis a prolific pest of cereal crops worldwide, controlled effectively with pyrethroid insecticides. However, the classic knock down resistance (kdr) mutation, L1014F on theS. avenaesodium channel gene, has been identified as the cause of the recently observed heterozygous (kdr-SR) resistance in the SA3 grain aphid super-clone. Results indicate that the kdr-SR SA3 clone can survive pyrethroid exposure above twice the normal field rate, continuing to reproduce thereafter. Additionally, the SA3 clone was found to be capable of producing sexual oviparous morphs, able to lay eggs following pyrethroid exposure. This demonstrates that possession of the L1014F mutation does not preclude the capacity to produce sexual morphs. This makes the adoption of an effective resistance management strategy imperative, within a wider integrated pest management (IPM) approach to control grain aphid.

scholarly journals Molecular Insight into the Knockdown Resistance (kdr) in the Voltage Gated Sodium Channel (vgsc) Gene of the Main Dengue Vector, Aedes aegypti (Diptera: Culicidae) and the Discovery of Novel Regional Specific Point Mutation A1007G in Malaysia.

2020 ◽  
Author(s):  
Mas Azlin M. Akhir ◽  
Mustafa F. F. Wajidi ◽  
Sébastien Lavoué ◽  
Ghows Azzam ◽  
Izhan Shahrin Jaafar ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Sodium Channel ◽  
Vector Control ◽  
Environmental Changes ◽  
Resistance Mechanism ◽  
Point Mutations ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Pirimiphos Methyl

Abstract Background: Characterization of the insecticide resistance mechanism imparts the society with the information on the evolutionary process involved in the adaptation of Aedes aegypti mosquito to environmental changes. Investigating the phenotypic status of the target mosquitoes, their resistance level as well as elucidating the genotypic profile provides information about the involvement of insecticide resistance mechanism, in terms of portraying the evolution of resistance in the field, to eventually managing vector control programs. In this current study, we investigated the quantification responses for the phenotypic and genotypic resistance of Ae. aegypti population from different states in Malaysia. Methods: We tested insecticide susceptibility status of adult Ae. aegypti from populations of States of Penang, Selangor and Kelantan (Peninsular Malaysia) against permethrin 0.25% and pirimiphos-methyl 0.25% through WHO bioassay kit. Permethrin-resistant and permethrin susceptible samples were then genotyped for domains II and III in the voltage gated sodium channel (vgsc) gene using allele specific PCR (AS-PCR) for the presence of diagnostic single nucleotide mutations. AS-PCR results were then validated in sequencing these two domains to identify any possible additional point mutations. Results: Adult WHO bioassay revealed that populations of Ae. aegypti from these three states were highly resistant towards Permethrin 0.25% and Pirimiphos-methyl 0.25%. Genotyping results showed that three knockdown (kdr) mutations (i.e. S989P, V1016G and F1534C) were associated with pyrethroid resistance in these populations. We also report for the first time the presence of the A1007G mutation in Malaysian populations of Ae. aegypti.Conclusions: This study brings an insight on the occurrence and association of point mutations with insecticide resistance in Malaysian populations of Ae. aegypti. The results reveal the widespread of several kdr mutations in the field with the consequence to compromise the use of pyrethroid insecticides in vector control programmes. Knowledge on the distribution of target site resistance throughout Malaysia is vital to ensure the success of the insecticide-based vector control program.

scholarly journals Modelling insecticide-binding sites in the voltage-gated sodium channel

2006 ◽  
Vol 396 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Andrias O. O'Reilly ◽  
Bhupinder P. S. Khambay ◽  
Martin S. Williamson ◽  
Linda M. Field ◽  
B. A. WAllace ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Binding Sites ◽  
Homology Model ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Arthropod Species ◽  
State Dependent ◽  
Binding Cavity

A homology model of the housefly voltage-gated sodium channel was developed to predict the location of binding sites for the insecticides fenvalerate, a synthetic pyrethroid, and DDT an early generation organochlorine. The model successfully addresses the state-dependent affinity of pyrethroid insecticides, their mechanism of action and the role of mutations in the channel that are known to confer insecticide resistance. The sodium channel was modelled in an open conformation with the insecticide-binding site located in a hydrophobic cavity delimited by the domain II S4-S5 linker and the IIS5 and IIIS6 helices. The binding cavity is predicted to be accessible to the lipid bilayer and therefore to lipid-soluble insecticides. The binding of insecticides and the consequent formation of binding contacts across different channel elements could stabilize the channel when in an open state, which is consistent with the prolonged sodium tail currents induced by pyrethroids and DDT. In the closed state, the predicted alternative positioning of the domain II S4-S5 linker would result in disruption of pyrethroid-binding contacts, consistent with the observation that pyrethroids have their highest affinity for the open channel. The model also predicts a key role for the IIS5 and IIIS6 helices in insecticide binding. Some of the residues on the helices that form the putative binding contacts are not conserved between arthropod and non-arthropod species, which is consistent with their contribution to insecticide species selectivity. Additional binding contacts on the II S4-S5 linker can explain the higher potency of pyrethroid insecticides compared with DDT.

scholarly journals Loss of pyrethroid resistance in newly established laboratory colonies of Aedes aegypti

2019 ◽  
Author(s):  
Farah Z. Vera-Maloof ◽  
Karla Saavedra-Rodriguez ◽  
Rosa P. Penilla-Navarro ◽  
Americo D. Rodriguez-Ramirez ◽  
Felipe Dzul ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Natural Populations ◽  
Resistance Management ◽  
Target Site ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Frequent Use

AbstractBackgroundResistance to pyrethroid insecticides in Aedes aegypti has become widespread after almost two decades of their frequent use to reduce arbovirus transmission. Despite this, use of pyrethroids continues because they are relatively inexpensive and because of their low human toxicity. Resistance management has been proposed as a means to retain the use of pyrethroids in natural populations. A key component of resistance management assumes that there is a negative fitness associated with resistance alleles so that when insecticides are removed, resistance alleles will decline in frequency. At least three studies in Ae. aegypti have demonstrated a decrease in pyrethroid resistance once the insecticide is removed.Methods/Principal FindingsThe present study aims to evaluate variation in the loss of pyrethroid resistance among newly established laboratory populations of Ae. aegypti from Mexico. Eight field collections were maintained for up to eight generations and we recorded changes in the frequencies of mutations at the V1,016I locus and at the F1,534C locus in the voltage gated sodium channel (VGSC) gene. I1,016 and C1,534 confer resistance. We also examined resistance ratios (RR) with type 1 and 2 pyrethroids.Conclusions/SignificanceWe demonstrate that, in general, the frequency of the Ae. aegypti pyrethroid resistance alleles I1,016 and C1,534 decline when they are freed from pyrethroid pressure in the laboratory. However, the pattern of decline is strain dependent. In agreement with earlier studies, RR was positively correlated with I1,016 resistant allele frequencies and showed significant protection against permethrin, and deltamethrin whereas F1534C showed protection against permethrin but not against deltamethrin.Author SummaryThe author is interested in the evolution of genes that confer resistance to insecticides, especially when this evolution affects binding of insecticides to their target site. The Voltage Gated Sodium Channel gene represents an excellent opportunity to understand how mutations at the target site(s) affect the evolution of resistance in many different pest insect species including Aedes aegypti, the primary vector of Dengue Virus, Yellow Fever, Zika and Chikungunya arboviruses.

The Voltage-Gated Sodium Channel Nav1.2 Contributes to Neurodegeneration in an Animal Model of Multiple Sclerosis

2016 ◽  
Vol 47 (S 01) ◽  
Author(s):  
W. Fazeli ◽  
B. Schattling ◽  
B. Engeland ◽  
M. Friese ◽  
D. Isbrand
Keyword(s):  
Multiple Sclerosis ◽  
Animal Model ◽  
Sodium Channel ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated
Sign in / Sign up

Export Citation Format

Share Document